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Thermolysin

Thermostable Proteinase with High Digest Temperature; Better Denaturation, Digestion of Proteolytically Resistant Proteins

  • Cleaves at the N-terminus of Leu, Phe, Val, Ile, Ala, Met
  • Optimal digestion temperature is 65–85°C
  • Optimal activity at pH 5.0–8.5

Size

Catalog number selected: V4001

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Thermolysin
25mg
$ 50.00
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Active at High Digestion Temperatures

Thermolysin is a thermostable metalloproteinase. The high digestion temperature stability of this enzyme may be useful as an alternative to denaturants to improve digestion of proteolytically resistant proteins. Thermolysin preferentially cleaves at the N-terminus of the hydrophobic residues leucine, phenylalanine, valine, isoleucine, alanine and methionine. The optimal digestion temperature range is 65–85°C. Thermolysin activity is optimal at pH 5.0–8.5.

Applications

  • Peptide mapping
  • Protein identification
  • Characterization of post-translational modifications
10439MA.eps
Cut sites for Elastase, Pepsin and Thermolysin.

Protocols

Specifications

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Item Part # Size

Thermolysin

V400A 1 x 25mg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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