Active at High Digestion Temperatures
Thermolysin is a thermostable metalloproteinase. The high digestion temperature stability of this enzyme may be useful as an alternative to denaturants to improve digestion of proteolytically resistant proteins. Thermolysin preferentially cleaves at the N-terminus of the hydrophobic residues leucine, phenylalanine, valine, isoleucine, alanine and methionine. The optimal digestion temperature range is 65–85°C. Thermolysin activity is optimal at pH 5.0–8.5.
Applications
- Peptide mapping
- Protein identification
- Characterization of post-translational modifications