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Lys-C, Mass Spec Grade, and Lys-N, Mass Spec Grade

Proteases for Challenging Digestions

  • Active under strong denaturing conditions
  • Choice of N-terminal (Lys-N) or C-terminal (Lys-C) lysine cleavage
  • Generates longer peptides than with tryptic digests

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Catalog number selected: VA1170

$ 400.00
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Lys-C, Mass Spec Grade, and Lys-N, Mass Spec Grade
Lys-C/20µg
$ 400.00
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Change Configuration
Illustration of LysN and LysC Digestion

Active Under Strong Denaturing Conditions

Endoproteinase Lys-C, Mass Spec Grade, is a highly-purified serine protease that hydrolyzes specifically at the carboxyl side of lysines. Lys-C retains proteolytic activity under strong protein denaturing conditions such as 8M urea, which can be used to improve digestion of proteolytically resistant proteins. Lys-C, Mass Spec Grade, has optimal activity in the range of pH 7.0—9.0. This protease can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. 

Endoproteinase Lys-N, Mass Spec Grade, is a zinc metalloprotease that cleaves at the amino side of lysines. Lys-N, Mass Spec Grade, retains proteolytic activity under strong protein denaturing conditions such as 8M urea, which can be used to improve digestion of proteolytically resistant proteins. Charged amino-terminal peptide fragments generated by Lys-N, Mass Spec Grade, are useful for de novo sequencing with ETD fragmentation techniques.

Digestion Under Strong Denaturing Conditions

Image of digestion under strong denaturing conditions.
Panel A. One-step digestion under strong denaturing conditions. Panel B. Two-step digestion using strong denaturing conditions for the first step and diluting for trypsin digestion in the second step.

Native Lys-C Interchangeable with Recombinant Lys-C

Comparison of recombinant and native Lys-C digestions.
Comparison of lysine-specific cleavages. Mass Spec-Compatible Yeast Protein Extract, Intact (Cat.# V7341) was digested with either rLys-C (P. aeruginosa; Cat.# V1671) or native Lys-C (Lysobacter sp.; Cat.# VA1170)) at a ratio of 1:100.

Specifications

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What's in the box?

Item Part # Size

Lys-C Mass Spec Grade

VA117A 1 × 20μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Specifications

You are viewing: VA1180 Change Configuration

What's in the box?

Item Part # Size

Lys-N Mass Spec Grade

VA118A 1 × 20μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB
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