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Chroma-Glo™ Luciferase Assay System

We are sorry, but this product is discontinued.

For more information, contact Technical Service.

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This product is discontinued
Chroma-Glo™ Luciferase Assay System

Dual-Color Luminescence Detection with Fewer Assay Steps

The Chroma-Glo™ Luciferase Assay System and the Chroma-Luc™ Vectors provide a method to generate red and green (dual-color) luminescence from a single sample upon a single-reagent addition. Filtered measurement of the dual-color luminescence produced by the Chroma-Luc™ luciferases permits each reporter to be measured independently and virtually simultaneously.

The Chroma-Glo™ Assay is in a homogeneous format that generates luminescence with >30-minute signal half-lives for each of the Chroma-Luc™ luciferases, thereby enabling the processing of many plates without prior sample handling.

The high-homology Chroma-Luc™ luciferases can be used to establish an ideal internal control for normalizing cytotoxicity in downregulation applications and for decreasing inter- and intrasample variability. You can also use the reporters to multiplex experimental reporters to increase the data content from cell-based assays.

Assay Advantages

  • Increase assay accuracy and precision through normalization, or use both reporters to multiplex experimental measurements. Use filters to spectrally separate the luminescent signals.
  • Establish the ideal control or multiplexed system. Use the high-homology red and green luciferases to minimize potential RNA and protein effects on reporter expression.
  • Use the stable luminescence for batch or continuous processing of multiple plates.
  • Fewer assay steps. Simply add Chroma-Luc™ Reagent directly to cells in medium, then measure.ewe
  • Automation-friendly. Validated automated methods are available at: www.promega.com/automethods/

Specifications

You are viewing: E4910 Change Configuration

What's in the box?

Item Part # Size

Chroma-Glo™ Luciferase Assay Substrate

E483A 1 × 1 vial

Chroma-Glo™ Luciferase Assay Buffer

E484A 1 × 10ml

SDS

Choose language:

Download SDSPDF (136 KB) – English (United States)

Certificate of Analysis

See all certificates for E4910

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Specifications

You are viewing: E4920 Change Configuration

What's in the box?

Item Part # Size

Chroma-Glo™ Luciferase Assay Substrate

E483B 1 × 1 vial

Chroma-Glo™ Luciferase Assay Buffer

E484B 1 × 100ml

SDS

Choose language:

Download SDSPDF (136 KB) – English (United States)

Certificate of Analysis

See all certificates for E4920

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

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