pGL4.41[luc2P/HSE/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGL4.41[luc2P/HSE/Hygro] Vector sequence reference points: Base pairs 6045 Synthetic polyadenylation signal sequence 105-153 Synthetic poly(A) signal/transcriptional pause region 105-258 Reporter vector primer 3 (RVprimer3) binding region 207-226 HSE response element 285-325 Minimal promoter 371-401 luc2P reporter gene 434-2209 SV40 late poly(A) region 2249-2470 SV40 early enhancer/promoter 2518-2936 Synthetic hygromycin (HygR) coding region 2961-3998 Synthetic polyadenylation signal sequence 4022-4070 Reporter vector primer 4 (RVprimer4) binding region 4137-4156 ColE1-derived plasmid replication origin 4394 Synthetic beta-lactamase(AmpR) coding region 5185-6045 .. 1 ACTCGTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TACTAGTACG 51 TCTCTCAAGG ATAAGTAAGT AATATTAAGG TACGGGAGGT ATTGGACAGG 101 CCGCAATAAA ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT 151 GTGAATCGAT AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA 201 AACAAACTAG CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC 251 ATTTCTCTGG CCTAACTGGC CGGTACCTGA GCTCCTGGAA GATTCTAGAA 301 CGTTCTGGAA GATTCTAGAA CGTTCCTCGA GGATATCAAG ATCTGGCCTC 351 GGCGGCCAAG CTTAGACACT AGAGGGTATA TAATGGAAGC TCGACTTCCA 401 GCTTGGCAAT CCGGTACTGT TGGTAAAGCC ACCATGGAAG ATGCCAAAAA 451 CATTAAGAAG GGCCCAGCGC CATTCTACCC ACTCGAAGAC GGGACCGCCG 501 GCGAGCAGCT GCACAAAGCC ATGAAGCGCT ACGCCCTGGT GCCCGGCACC 551 ATCGCCTTTA CCGACGCACA TATCGAGGTG GACATTACCT ACGCCGAGTA 601 CTTCGAGATG AGCGTTCGGC TGGCAGAAGC TATGAAGCGC TATGGGCTGA 651 ATACAAACCA TCGGATCGTG GTGTGCAGCG AGAATAGCTT GCAGTTCTTC 701 ATGCCCGTGT TGGGTGCCCT GTTCATCGGT GTGGCTGTGG CCCCAGCTAA 751 CGACATCTAC AACGAGCGCG AGCTGCTGAA CAGCATGGGC ATCAGCCAGC 801 CCACCGTCGT ATTCGTGAGC AAGAAAGGGC TGCAAAAGAT CCTCAACGTG 851 CAAAAGAAGC TACCGATCAT ACAAAAGATC ATCATCATGG ATAGCAAGAC 901 CGACTACCAG GGCTTCCAAA GCATGTACAC CTTCGTGACT TCCCATTTGC 951 CACCCGGCTT CAACGAGTAC GACTTCGTGC CCGAGAGCTT CGACCGGGAC 1001 AAAACCATCG CCCTGATCAT GAACAGTAGT GGCAGTACCG GATTGCCCAA 1051 GGGCGTAGCC CTACCGCACC GCACCGCTTG TGTCCGATTC AGTCATGCCC 1101 GCGACCCCAT CTTCGGCAAC CAGATCATCC CCGACACCGC TATCCTCAGC 1151 GTGGTGCCAT TTCACCACGG CTTCGGCATG TTCACCACGC TGGGCTACTT 1201 GATCTGCGGC TTTCGGGTCG TGCTCATGTA CCGCTTCGAG GAGGAGCTAT 1251 TCTTGCGCAG CTTGCAAGAC TATAAGATTC AATCTGCCCT GCTGGTGCCC 1301 ACACTATTTA GCTTCTTCGC TAAGAGCACT CTCATCGACA AGTACGACCT 1351 AAGCAACTTG CACGAGATCG CCAGCGGCGG GGCGCCGCTC AGCAAGGAGG 1401 TAGGTGAGGC CGTGGCCAAA CGCTTCCACC TACCAGGCAT CCGCCAGGGC 1451 TACGGCCTGA CAGAAACAAC CAGCGCCATT CTGATCACCC CCGAAGGGGA 1501 CGACAAGCCT GGCGCAGTAG GCAAGGTGGT GCCCTTCTTC GAGGCTAAGG 1551 TGGTGGACTT GGACACCGGT AAGACACTGG GTGTGAACCA GCGCGGCGAG 1601 CTGTGCGTCC GTGGCCCCAT GATCATGAGC GGCTACGTTA ACAACCCCGA 1651 GGCTACAAAC GCTCTCATCG ACAAGGACGG CTGGCTGCAC AGCGGCGACA 1701 TCGCCTACTG GGACGAGGAC GAGCACTTCT TCATCGTGGA CCGGCTGAAG 1751 AGCCTGATCA AATACAAGGG CTACCAGGTA GCCCCAGCCG AACTGGAGAG 1801 CATCCTGCTG CAACACCCCA ACATCTTCGA CGCCGGGGTC GCCGGCCTGC 1851 CCGACGACGA TGCCGGCGAG CTGCCCGCCG CAGTCGTCGT GCTGGAACAC 1901 GGTAAAACCA TGACCGAGAA GGAGATCGTG GACTATGTGG CCAGCCAGGT 1951 TACAACCGCC AAGAAGCTGC GCGGTGGTGT TGTGTTCGTG GACGAGGTGC 2001 CTAAAGGACT GACCGGCAAG TTGGACGCCC GCAAGATCCG CGAGATTCTC 2051 ATTAAGGCCA AGAAGGGCGG CAAGATCGCC GTGAATTCTC ACGGCTTCCC 2101 TCCCGAGGTG GAGGAGCAGG CCGCCGGCAC CCTGCCCATG AGCTGCGCCC 2151 AGGAGAGCGG CATGGATAGA CACCCTGCTG CTTGCGCCAG CGCCAGGATC 2201 AACGTCTAAG GCCGCGACTC TAGAGTCGGG GCGGCCGGCC GCTTCGAGCA 2251 GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA 2301 GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG 2351 TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT 2401 TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA 2451 AAACCTCTAC AAATGTGGTA AAATCGATAA GGATCCGTTT GCGTATTGGG 2501 CGCTCTTCCG CTGATCTGCG CAGCACCATG GCCTGAAATA ACCTCTGAAA 2551 GAGGAACTTG GTTAGCTACC TTCTGAGGCG GAAAGAACCA GCTGTGGAAT 2601 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG 2651 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC 2701 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 2751 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC 2801 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG 2851 CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 2901 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCGATT CTTCTGACAC 2951 TAGCGCCACC ATGAAGAAGC CCGAACTCAC CGCTACCAGC GTTGAAAAAT 3001 TTCTCATCGA GAAGTTCGAC AGTGTGAGCG ACCTGATGCA GTTGTCGGAG 3051 GGCGAAGAGA GCCGAGCCTT CAGCTTCGAT GTCGGCGGAC GCGGCTATGT 3101 ACTGCGGGTG AATAGCTGCG CTGATGGCTT CTACAAAGAC CGCTACGTGT 3151 ACCGCCACTT CGCCAGCGCT GCACTACCCA TCCCCGAAGT GTTGGACATC 3201 GGCGAGTTCA GCGAGAGCCT GACATACTGC ATCAGTAGAC GCGCCCAAGG 3251 CGTTACTCTC CAAGACCTCC CCGAAACAGA GCTGCCTGCT GTGTTACAGC 3301 CTGTCGCCGA AGCTATGGAT GCTATTGCCG CCGCCGACCT CAGTCAAACC 3351 AGCGGCTTCG GCCCATTCGG GCCCCAAGGC ATCGGCCAGT ACACAACCTG 3401 GCGGGATTTC ATTTGCGCCA TTGCTGATCC CCATGTCTAC CACTGGCAGA 3451 CCGTGATGGA CGACACCGTG TCCGCCAGCG TAGCTCAAGC CCTGGACGAA 3501 CTGATGCTGT GGGCCGAAGA CTGTCCCGAG GTGCGCCACC TCGTCCATGC 3551 CGACTTCGGC AGCAACAACG TCCTGACCGA CAACGGCCGC ATCACCGCCG 3601 TAATCGACTG GTCCGAAGCT ATGTTCGGGG ACAGTCAGTA CGAGGTGGCC 3651 AACATCTTCT TCTGGCGGCC CTGGCTGGCT TGCATGGAGC AGCAGACTCG 3701 CTACTTCGAG CGCCGGCATC CCGAGCTGGC CGGCAGCCCT CGTCTGCGAG 3751 CCTACATGCT GCGCATCGGC CTGGATCAGC TCTACCAGAG CCTCGTGGAC 3801 GGCAACTTCG ACGATGCTGC CTGGGCTCAA GGCCGCTGCG ATGCCATCGT 3851 CCGCAGCGGG GCCGGCACCG TCGGTCGCAC ACAAATCGCT CGCCGGAGCG 3901 CAGCCGTATG GACCGACGGC TGCGTCGAGG TGCTGGCCGA CAGCGGCAAC 3951 CGCCGGCCCA GTACACGACC GCGCGCTAAG GAGGTAGGTC GAGTTTAAAC 4001 TCTAGAACCG GTCATGGCCG CAATAAAATA TCTTTATTTT CATTACATCT 4051 GTGTGTTGGT TTTTTGTGTG TTCGAACTAG ATGCTGTCGA CCGATGCCCT 4101 TGAGAGCCTT CAACCCAGTC AGCTCCTTCC GGTGGGCGCG GGGCATGACT 4151 ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC TCGTAGGACA 4201 GGTGCCGGCA GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG 4251 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 4301 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 4351 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC 4401 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 4451 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 4501 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC 4551 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 4601 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 4651 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT 4701 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 4751 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 4801 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT 4851 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 4901 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA 4951 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC 5001 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 5051 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 5101 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 5151 GACAGCGGCC GCAAATGCTA AACCACTGCA GTGGTTACCA GTGCTTGATC 5201 AGTGAGGCAC CGATCTCAGC GATCTGCCTA TTTCGTTCGT CCATAGTGGC 5251 CTGACTCCCC GTCGTGTAGA TCACTACGAT TCGTGAGGGC TTACCATCAG 5301 GCCCCAGCGC AGCAATGATG CCGCGAGAGC CGCGTTCACC GGCCCCCGAT 5351 TTGTCAGCAA TGAACCAGCC AGCAGGGAGG GCCGAGCGAA GAAGTGGTCC 5401 TGCTACTTTG TCCGCCTCCA TCCAGTCTAT GAGCTGCTGT CGTGATGCTA 5451 GAGTAAGAAG TTCGCCAGTG AGTAGTTTCC GAAGAGTTGT GGCCATTGCT 5501 ACTGGCATCG TGGTATCACG CTCGTCGTTC GGTATGGCTT CGTTCAACTC 5551 TGGTTCCCAG CGGTCAAGCC GGGTCACATG ATCACCCATA TTATGAAGAA 5601 ATGCAGTCAG CTCCTTAGGG CCTCCGATCG TTGTCAGAAG TAAGTTGGCC 5651 GCGGTGTTGT CGCTCATGGT AATGGCAGCA CTACACAATT CTCTTACCGT 5701 CATGCCATCC GTAAGATGCT TTTCCGTGAC CGGCGAGTAC TCAACCAAGT 5751 CGTTTTGTGA GTAGTGTATA CGGCGACCAA GCTGCTCTTG CCCGGCGTCT 5801 ATACGGGACA ACACCGCGCC ACATAGCAGT ACTTTGAAAG TGCTCATCAT 5851 CGGGAATCGT TCTTCGGGGC GGAAAGACTC AAGGATCTTG CCGCTATTGA 5901 GATCCAGTTC GATATAGCCC ACTCTTGCAC CCAGTTGATC TTCAGCATCT 5951 TTTACTTTCA CCAGCGTTTC GGGGTGTGCA AAAACAGGCA AGCAAAATGC 6001 CGCAAAGAAG GGAATGAGTG CGACACGAAA ATGTTGGATG CTCAT Sequence file prepared 6-June-2012.