pGL4.42[luc2P/HRE/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGL4.42[luc2P/HRE/Hygro] Vector sequence reference points: Base pairs 6080 Synthetic polyadenylation signal sequence 105-153 Synthetic poly(A) signal/transcriptional pause region 105-258 Reporter vector primer 3 (RVprimer3) binding region 207-226 HRE response element 285-360 Minimal promoter 406-436 luc2P reporter gene 469-2244 SV40 late poly(A) region 2284-2505 SV40 early enhancer/promoter 2553-2971 Synthetic hygromycin (HygR) coding region 2996-4033 Synthetic polyadenylation signal sequence 4057-4105 Reporter vector primer 4 (RVprimer4) binding region 4172-4191 ColE1-derived plasmid replication origin 4429 Synthetic beta-lactamase(AmpR) coding region 5220-6080 .. 1 ACTCGTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TACTAGTACG 51 TCTCTCAAGG ATAAGTAAGT AATATTAAGG TACGGGAGGT ATTGGACAGG 101 CCGCAATAAA ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT 151 GTGAATCGAT AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA 201 AACAAACTAG CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC 251 ATTTCTCTGG CCTAACTGGC CGGTACCTGA GCTCGTGACT ACGTGCTGCC 301 TAGGTGACTA CGTGCTGCCT AGGTGACTAC GTGCTGCCTA GGTGACTACG 351 TGCTGCCTAG CTCGAGGATA TCAAGATCTG GCCTCGGCGG CCAAGCTTAG 401 ACACTAGAGG GTATATAATG GAAGCTCGAC TTCCAGCTTG GCAATCCGGT 451 ACTGTTGGTA AAGCCACCAT GGAAGATGCC AAAAACATTA AGAAGGGCCC 501 AGCGCCATTC TACCCACTCG AAGACGGGAC CGCCGGCGAG CAGCTGCACA 551 AAGCCATGAA GCGCTACGCC CTGGTGCCCG GCACCATCGC CTTTACCGAC 601 GCACATATCG AGGTGGACAT TACCTACGCC GAGTACTTCG AGATGAGCGT 651 TCGGCTGGCA GAAGCTATGA AGCGCTATGG GCTGAATACA AACCATCGGA 701 TCGTGGTGTG CAGCGAGAAT AGCTTGCAGT TCTTCATGCC CGTGTTGGGT 751 GCCCTGTTCA TCGGTGTGGC TGTGGCCCCA GCTAACGACA TCTACAACGA 801 GCGCGAGCTG CTGAACAGCA TGGGCATCAG CCAGCCCACC GTCGTATTCG 851 TGAGCAAGAA AGGGCTGCAA AAGATCCTCA ACGTGCAAAA GAAGCTACCG 901 ATCATACAAA AGATCATCAT CATGGATAGC AAGACCGACT ACCAGGGCTT 951 CCAAAGCATG TACACCTTCG TGACTTCCCA TTTGCCACCC GGCTTCAACG 1001 AGTACGACTT CGTGCCCGAG AGCTTCGACC GGGACAAAAC CATCGCCCTG 1051 ATCATGAACA GTAGTGGCAG TACCGGATTG CCCAAGGGCG TAGCCCTACC 1101 GCACCGCACC GCTTGTGTCC GATTCAGTCA TGCCCGCGAC CCCATCTTCG 1151 GCAACCAGAT CATCCCCGAC ACCGCTATCC TCAGCGTGGT GCCATTTCAC 1201 CACGGCTTCG GCATGTTCAC CACGCTGGGC TACTTGATCT GCGGCTTTCG 1251 GGTCGTGCTC ATGTACCGCT TCGAGGAGGA GCTATTCTTG CGCAGCTTGC 1301 AAGACTATAA GATTCAATCT GCCCTGCTGG TGCCCACACT ATTTAGCTTC 1351 TTCGCTAAGA GCACTCTCAT CGACAAGTAC GACCTAAGCA ACTTGCACGA 1401 GATCGCCAGC GGCGGGGCGC CGCTCAGCAA GGAGGTAGGT GAGGCCGTGG 1451 CCAAACGCTT CCACCTACCA GGCATCCGCC AGGGCTACGG CCTGACAGAA 1501 ACAACCAGCG CCATTCTGAT CACCCCCGAA GGGGACGACA AGCCTGGCGC 1551 AGTAGGCAAG GTGGTGCCCT TCTTCGAGGC TAAGGTGGTG GACTTGGACA 1601 CCGGTAAGAC ACTGGGTGTG AACCAGCGCG GCGAGCTGTG CGTCCGTGGC 1651 CCCATGATCA TGAGCGGCTA CGTTAACAAC CCCGAGGCTA CAAACGCTCT 1701 CATCGACAAG GACGGCTGGC TGCACAGCGG CGACATCGCC TACTGGGACG 1751 AGGACGAGCA CTTCTTCATC GTGGACCGGC TGAAGAGCCT GATCAAATAC 1801 AAGGGCTACC AGGTAGCCCC AGCCGAACTG GAGAGCATCC TGCTGCAACA 1851 CCCCAACATC TTCGACGCCG GGGTCGCCGG CCTGCCCGAC GACGATGCCG 1901 GCGAGCTGCC CGCCGCAGTC GTCGTGCTGG AACACGGTAA AACCATGACC 1951 GAGAAGGAGA TCGTGGACTA TGTGGCCAGC CAGGTTACAA CCGCCAAGAA 2001 GCTGCGCGGT GGTGTTGTGT TCGTGGACGA GGTGCCTAAA GGACTGACCG 2051 GCAAGTTGGA CGCCCGCAAG ATCCGCGAGA TTCTCATTAA GGCCAAGAAG 2101 GGCGGCAAGA TCGCCGTGAA TTCTCACGGC TTCCCTCCCG AGGTGGAGGA 2151 GCAGGCCGCC GGCACCCTGC CCATGAGCTG CGCCCAGGAG AGCGGCATGG 2201 ATAGACACCC TGCTGCTTGC GCCAGCGCCA GGATCAACGT CTAAGGCCGC 2251 GACTCTAGAG TCGGGGCGGC CGGCCGCTTC GAGCAGACAT GATAAGATAC 2301 ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT 2351 TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT 2401 GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT 2451 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG 2501 TGGTAAAATC GATAAGGATC CGTTTGCGTA TTGGGCGCTC TTCCGCTGAT 2551 CTGCGCAGCA CCATGGCCTG AAATAACCTC TGAAAGAGGA ACTTGGTTAG 2601 CTACCTTCTG AGGCGGAAAG AACCAGCTGT GGAATGTGTG TCAGTTAGGG 2651 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA 2701 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG 2751 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 2801 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC 2851 TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG 2901 CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 2951 CTAGGCTTTT GCAAAAAGCT CGATTCTTCT GACACTAGCG CCACCATGAA 3001 GAAGCCCGAA CTCACCGCTA CCAGCGTTGA AAAATTTCTC ATCGAGAAGT 3051 TCGACAGTGT GAGCGACCTG ATGCAGTTGT CGGAGGGCGA AGAGAGCCGA 3101 GCCTTCAGCT TCGATGTCGG CGGACGCGGC TATGTACTGC GGGTGAATAG 3151 CTGCGCTGAT GGCTTCTACA AAGACCGCTA CGTGTACCGC CACTTCGCCA 3201 GCGCTGCACT ACCCATCCCC GAAGTGTTGG ACATCGGCGA GTTCAGCGAG 3251 AGCCTGACAT ACTGCATCAG TAGACGCGCC CAAGGCGTTA CTCTCCAAGA 3301 CCTCCCCGAA ACAGAGCTGC CTGCTGTGTT ACAGCCTGTC GCCGAAGCTA 3351 TGGATGCTAT TGCCGCCGCC GACCTCAGTC AAACCAGCGG CTTCGGCCCA 3401 TTCGGGCCCC AAGGCATCGG CCAGTACACA ACCTGGCGGG ATTTCATTTG 3451 CGCCATTGCT GATCCCCATG TCTACCACTG GCAGACCGTG ATGGACGACA 3501 CCGTGTCCGC CAGCGTAGCT CAAGCCCTGG ACGAACTGAT GCTGTGGGCC 3551 GAAGACTGTC CCGAGGTGCG CCACCTCGTC CATGCCGACT TCGGCAGCAA 3601 CAACGTCCTG ACCGACAACG GCCGCATCAC CGCCGTAATC GACTGGTCCG 3651 AAGCTATGTT CGGGGACAGT CAGTACGAGG TGGCCAACAT CTTCTTCTGG 3701 CGGCCCTGGC TGGCTTGCAT GGAGCAGCAG ACTCGCTACT TCGAGCGCCG 3751 GCATCCCGAG CTGGCCGGCA GCCCTCGTCT GCGAGCCTAC ATGCTGCGCA 3801 TCGGCCTGGA TCAGCTCTAC CAGAGCCTCG TGGACGGCAA CTTCGACGAT 3851 GCTGCCTGGG CTCAAGGCCG CTGCGATGCC ATCGTCCGCA GCGGGGCCGG 3901 CACCGTCGGT CGCACACAAA TCGCTCGCCG GAGCGCAGCC GTATGGACCG 3951 ACGGCTGCGT CGAGGTGCTG GCCGACAGCG GCAACCGCCG GCCCAGTACA 4001 CGACCGCGCG CTAAGGAGGT AGGTCGAGTT TAAACTCTAG AACCGGTCAT 4051 GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT 4101 GTGTGTTCGA ACTAGATGCT GTCGACCGAT GCCCTTGAGA GCCTTCAACC 4151 CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT CGCCGCACTT 4201 ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC CGGCAGCGCT 4251 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG 4301 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 4351 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 4401 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC 4451 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 4501 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 4551 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 4601 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 4651 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 4701 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC 4751 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 4801 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 4851 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG 4901 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 4951 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 5001 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT 5051 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 5101 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 5151 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG CGGCCGCAAA 5201 TGCTAAACCA CTGCAGTGGT TACCAGTGCT TGATCAGTGA GGCACCGATC 5251 TCAGCGATCT GCCTATTTCG TTCGTCCATA GTGGCCTGAC TCCCCGTCGT 5301 GTAGATCACT ACGATTCGTG AGGGCTTACC ATCAGGCCCC AGCGCAGCAA 5351 TGATGCCGCG AGAGCCGCGT TCACCGGCCC CCGATTTGTC AGCAATGAAC 5401 CAGCCAGCAG GGAGGGCCGA GCGAAGAAGT GGTCCTGCTA CTTTGTCCGC 5451 CTCCATCCAG TCTATGAGCT GCTGTCGTGA TGCTAGAGTA AGAAGTTCGC 5501 CAGTGAGTAG TTTCCGAAGA GTTGTGGCCA TTGCTACTGG CATCGTGGTA 5551 TCACGCTCGT CGTTCGGTAT GGCTTCGTTC AACTCTGGTT CCCAGCGGTC 5601 AAGCCGGGTC ACATGATCAC CCATATTATG AAGAAATGCA GTCAGCTCCT 5651 TAGGGCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCGGT GTTGTCGCTC 5701 ATGGTAATGG CAGCACTACA CAATTCTCTT ACCGTCATGC CATCCGTAAG 5751 ATGCTTTTCC GTGACCGGCG AGTACTCAAC CAAGTCGTTT TGTGAGTAGT 5801 GTATACGGCG ACCAAGCTGC TCTTGCCCGG CGTCTATACG GGACAACACC 5851 GCGCCACATA GCAGTACTTT GAAAGTGCTC ATCATCGGGA ATCGTTCTTC 5901 GGGGCGGAAA GACTCAAGGA TCTTGCCGCT ATTGAGATCC AGTTCGATAT 5951 AGCCCACTCT TGCACCCAGT TGATCTTCAG CATCTTTTAC TTTCACCAGC 6001 GTTTCGGGGT GTGCAAAAAC AGGCAAGCAA AATGCCGCAA AGAAGGGAAT 6051 GAGTGCGACA CGAAAATGTT GGATGCTCAT Sequence file prepared 4-June-2012.