pGL4.48[luc2P/SBE/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGL4.48[luc2P/SBE/Hygro] Vector sequence reference points: Base pairs 6052 Synthetic polyadenylation signal sequence 105-153 Synthetic poly(A) signal/transcriptional pause region 105-258 Reporter vector primer 3 (RVprimer3) binding region 207-226 SBE response element 285-332 Minimal promoter 378-408 luc2P reporter gene 441-2216 SV40 late poly(A) region 2256-2477 SV40 early enhancer/promoter 2525-2943 Synthetic hygromycin (HygR) coding region 2968-4005 Synthetic polyadenylation signal sequence 4029-4077 Reporter vector primer 4 (RVprimer4) binding region 4144-4163 ColE1-derived plasmid replication origin 4401 Synthetic beta-lactamase(AmpR) coding region 5192-6052 .. 1 ACTCGTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TACTAGTACG 51 TCTCTCAAGG ATAAGTAAGT AATATTAAGG TACGGGAGGT ATTGGACAGG 101 CCGCAATAAA ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT 151 GTGAATCGAT AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA 201 AACAAACTAG CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC 251 ATTTCTCTGG CCTAACTGGC CGGTACCTGA GCTCAGTATG TCTAGACTGA 301 AGTATGTCTA GACTGAAGTA TGTCTAGACT GACTCGAGGA TATCAAGATC 351 TGGCCTCGGC GGCCAAGCTT AGACACTAGA GGGTATATAA TGGAAGCTCG 401 ACTTCCAGCT TGGCAATCCG GTACTGTTGG TAAAGCCACC ATGGAAGATG 451 CCAAAAACAT TAAGAAGGGC CCAGCGCCAT TCTACCCACT CGAAGACGGG 501 ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG CCCTGGTGCC 551 CGGCACCATC GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG 601 CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT 651 GGGCTGAATA CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA 701 GTTCTTCATG CCCGTGTTGG GTGCCCTGTT CATCGGTGTG GCTGTGGCCC 751 CAGCTAACGA CATCTACAAC GAGCGCGAGC TGCTGAACAG CATGGGCATC 801 AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC AAAAGATCCT 851 CAACGTGCAA AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA 901 GCAAGACCGA CTACCAGGGC TTCCAAAGCA TGTACACCTT CGTGACTTCC 951 CATTTGCCAC CCGGCTTCAA CGAGTACGAC TTCGTGCCCG AGAGCTTCGA 1001 CCGGGACAAA ACCATCGCCC TGATCATGAA CAGTAGTGGC AGTACCGGAT 1051 TGCCCAAGGG CGTAGCCCTA CCGCACCGCA CCGCTTGTGT CCGATTCAGT 1101 CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG ACACCGCTAT 1151 CCTCAGCGTG GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG 1201 GCTACTTGAT CTGCGGCTTT CGGGTCGTGC TCATGTACCG CTTCGAGGAG 1251 GAGCTATTCT TGCGCAGCTT GCAAGACTAT AAGATTCAAT CTGCCCTGCT 1301 GGTGCCCACA CTATTTAGCT TCTTCGCTAA GAGCACTCTC ATCGACAAGT 1351 ACGACCTAAG CAACTTGCAC GAGATCGCCA GCGGCGGGGC GCCGCTCAGC 1401 AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC CAGGCATCCG 1451 CCAGGGCTAC GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG 1501 AAGGGGACGA CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG 1551 GCTAAGGTGG TGGACTTGGA CACCGGTAAG ACACTGGGTG TGAACCAGCG 1601 CGGCGAGCTG TGCGTCCGTG GCCCCATGAT CATGAGCGGC TACGTTAACA 1651 ACCCCGAGGC TACAAACGCT CTCATCGACA AGGACGGCTG GCTGCACAGC 1701 GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA TCGTGGACCG 1751 GCTGAAGAGC CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC 1801 TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC CGGGGTCGCC 1851 GGCCTGCCCG ACGACGATGC CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT 1901 GGAACACGGT AAAACCATGA CCGAGAAGGA GATCGTGGAC TATGTGGCCA 1951 GCCAGGTTAC AACCGCCAAG AAGCTGCGCG GTGGTGTTGT GTTCGTGGAC 2001 GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA AGATCCGCGA 2051 GATTCTCATT AAGGCCAAGA AGGGCGGCAA GATCGCCGTG AATTCTCACG 2101 GCTTCCCTCC CGAGGTGGAG GAGCAGGCCG CCGGCACCCT GCCCATGAGC 2151 TGCGCCCAGG AGAGCGGCAT GGATAGACAC CCTGCTGCTT GCGCCAGCGC 2201 CAGGATCAAC GTCTAAGGCC GCGACTCTAG AGTCGGGGCG GCCGGCCGCT 2251 TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA 2301 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT 2351 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG 2401 CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA 2451 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGATAAGGA TCCGTTTGCG 2501 TATTGGGCGC TCTTCCGCTG ATCTGCGCAG CACCATGGCC TGAAATAACC 2551 TCTGAAAGAG GAACTTGGTT AGCTACCTTC TGAGGCGGAA AGAACCAGCT 2601 GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG 2651 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 2701 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 2751 TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA 2801 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 2851 ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA 2901 GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCGATTCTT 2951 CTGACACTAG CGCCACCATG AAGAAGCCCG AACTCACCGC TACCAGCGTT 3001 GAAAAATTTC TCATCGAGAA GTTCGACAGT GTGAGCGACC TGATGCAGTT 3051 GTCGGAGGGC GAAGAGAGCC GAGCCTTCAG CTTCGATGTC GGCGGACGCG 3101 GCTATGTACT GCGGGTGAAT AGCTGCGCTG ATGGCTTCTA CAAAGACCGC 3151 TACGTGTACC GCCACTTCGC CAGCGCTGCA CTACCCATCC CCGAAGTGTT 3201 GGACATCGGC GAGTTCAGCG AGAGCCTGAC ATACTGCATC AGTAGACGCG 3251 CCCAAGGCGT TACTCTCCAA GACCTCCCCG AAACAGAGCT GCCTGCTGTG 3301 TTACAGCCTG TCGCCGAAGC TATGGATGCT ATTGCCGCCG CCGACCTCAG 3351 TCAAACCAGC GGCTTCGGCC CATTCGGGCC CCAAGGCATC GGCCAGTACA 3401 CAACCTGGCG GGATTTCATT TGCGCCATTG CTGATCCCCA TGTCTACCAC 3451 TGGCAGACCG TGATGGACGA CACCGTGTCC GCCAGCGTAG CTCAAGCCCT 3501 GGACGAACTG ATGCTGTGGG CCGAAGACTG TCCCGAGGTG CGCCACCTCG 3551 TCCATGCCGA CTTCGGCAGC AACAACGTCC TGACCGACAA CGGCCGCATC 3601 ACCGCCGTAA TCGACTGGTC CGAAGCTATG TTCGGGGACA GTCAGTACGA 3651 GGTGGCCAAC ATCTTCTTCT GGCGGCCCTG GCTGGCTTGC ATGGAGCAGC 3701 AGACTCGCTA CTTCGAGCGC CGGCATCCCG AGCTGGCCGG CAGCCCTCGT 3751 CTGCGAGCCT ACATGCTGCG CATCGGCCTG GATCAGCTCT ACCAGAGCCT 3801 CGTGGACGGC AACTTCGACG ATGCTGCCTG GGCTCAAGGC CGCTGCGATG 3851 CCATCGTCCG CAGCGGGGCC GGCACCGTCG GTCGCACACA AATCGCTCGC 3901 CGGAGCGCAG CCGTATGGAC CGACGGCTGC GTCGAGGTGC TGGCCGACAG 3951 CGGCAACCGC CGGCCCAGTA CACGACCGCG CGCTAAGGAG GTAGGTCGAG 4001 TTTAAACTCT AGAACCGGTC ATGGCCGCAA TAAAATATCT TTATTTTCAT 4051 TACATCTGTG TGTTGGTTTT TTGTGTGTTC GAACTAGATG CTGTCGACCG 4101 ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG 4151 CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG 4201 TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 4251 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 4301 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 4351 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 4401 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 4451 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 4501 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 4551 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 4601 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 4651 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 4701 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 4751 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 4801 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 4851 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 4901 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 4951 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 5001 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 5051 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 5101 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 5151 TTGGTCTGAC AGCGGCCGCA AATGCTAAAC CACTGCAGTG GTTACCAGTG 5201 CTTGATCAGT GAGGCACCGA TCTCAGCGAT CTGCCTATTT CGTTCGTCCA 5251 TAGTGGCCTG ACTCCCCGTC GTGTAGATCA CTACGATTCG TGAGGGCTTA 5301 CCATCAGGCC CCAGCGCAGC AATGATGCCG CGAGAGCCGC GTTCACCGGC 5351 CCCCGATTTG TCAGCAATGA ACCAGCCAGC AGGGAGGGCC GAGCGAAGAA 5401 GTGGTCCTGC TACTTTGTCC GCCTCCATCC AGTCTATGAG CTGCTGTCGT 5451 GATGCTAGAG TAAGAAGTTC GCCAGTGAGT AGTTTCCGAA GAGTTGTGGC 5501 CATTGCTACT GGCATCGTGG TATCACGCTC GTCGTTCGGT ATGGCTTCGT 5551 TCAACTCTGG TTCCCAGCGG TCAAGCCGGG TCACATGATC ACCCATATTA 5601 TGAAGAAATG CAGTCAGCTC CTTAGGGCCT CCGATCGTTG TCAGAAGTAA 5651 GTTGGCCGCG GTGTTGTCGC TCATGGTAAT GGCAGCACTA CACAATTCTC 5701 TTACCGTCAT GCCATCCGTA AGATGCTTTT CCGTGACCGG CGAGTACTCA 5751 ACCAAGTCGT TTTGTGAGTA GTGTATACGG CGACCAAGCT GCTCTTGCCC 5801 GGCGTCTATA CGGGACAACA CCGCGCCACA TAGCAGTACT TTGAAAGTGC 5851 TCATCATCGG GAATCGTTCT TCGGGGCGGA AAGACTCAAG GATCTTGCCG 5901 CTATTGAGAT CCAGTTCGAT ATAGCCCACT CTTGCACCCA GTTGATCTTC 5951 AGCATCTTTT ACTTTCACCA GCGTTTCGGG GTGTGCAAAA ACAGGCAAGC 6001 AAAATGCCGC AAAGAAGGGA ATGAGTGCGA CACGAAAATG TTGGATGCTC 6051 AT Sequence file prepared 6-June-2012.