pGL4.45[luc2P/ISRE/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGL4.45[luc2P/ISRE/Hygro] Vector sequence reference points: Base pairs 6079 Synthetic polyadenylation signal sequence 105-153 Synthetic poly(A) signal/transcriptional pause region 105-258 Reporter vector primer 3 (RVprimer3) binding region 207-226 ISRE response element 285-359 Minimal promoter 405-435 luc2P reporter gene 468-2243 SV40 late poly(A) region 2283-2504 SV40 early enhancer/promoter 2552-2970 Synthetic hygromycin (HygR) coding region 2995-4032 Synthetic polyadenylation signal sequence 4056-4104 Reporter vector primer 4 (RVprimer4) binding region 4171-4190 ColE1-derived plasmid replication origin 4428 Synthetic beta-lactamase(AmpR) coding region 5219-6079 .. 1 ACTCGTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TACTAGTACG 51 TCTCTCAAGG ATAAGTAAGT AATATTAAGG TACGGGAGGT ATTGGACAGG 101 CCGCAATAAA ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT 151 GTGAATCGAT AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA 201 AACAAACTAG CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC 251 ATTTCTCTGG CCTAACTGGC CGGTACCTGA GCTCTAGTTT CACTTTCCCT 301 AGTTTCACTT TCCCTAGTTT CACTTTCCCT AGTTTCACTT TCCCTAGTTT 351 CACTTTCCCC TCGAGGATAT CAAGATCTGG CCTCGGCGGC CAAGCTTAGA 401 CACTAGAGGG TATATAATGG AAGCTCGACT TCCAGCTTGG CAATCCGGTA 451 CTGTTGGTAA AGCCACCATG GAAGATGCCA AAAACATTAA GAAGGGCCCA 501 GCGCCATTCT ACCCACTCGA AGACGGGACC GCCGGCGAGC AGCTGCACAA 551 AGCCATGAAG CGCTACGCCC TGGTGCCCGG CACCATCGCC TTTACCGACG 601 CACATATCGA GGTGGACATT ACCTACGCCG AGTACTTCGA GATGAGCGTT 651 CGGCTGGCAG AAGCTATGAA GCGCTATGGG CTGAATACAA ACCATCGGAT 701 CGTGGTGTGC AGCGAGAATA GCTTGCAGTT CTTCATGCCC GTGTTGGGTG 751 CCCTGTTCAT CGGTGTGGCT GTGGCCCCAG CTAACGACAT CTACAACGAG 801 CGCGAGCTGC TGAACAGCAT GGGCATCAGC CAGCCCACCG TCGTATTCGT 851 GAGCAAGAAA GGGCTGCAAA AGATCCTCAA CGTGCAAAAG AAGCTACCGA 901 TCATACAAAA GATCATCATC ATGGATAGCA AGACCGACTA CCAGGGCTTC 951 CAAAGCATGT ACACCTTCGT GACTTCCCAT TTGCCACCCG GCTTCAACGA 1001 GTACGACTTC GTGCCCGAGA GCTTCGACCG GGACAAAACC ATCGCCCTGA 1051 TCATGAACAG TAGTGGCAGT ACCGGATTGC CCAAGGGCGT AGCCCTACCG 1101 CACCGCACCG CTTGTGTCCG ATTCAGTCAT GCCCGCGACC CCATCTTCGG 1151 CAACCAGATC ATCCCCGACA CCGCTATCCT CAGCGTGGTG CCATTTCACC 1201 ACGGCTTCGG CATGTTCACC ACGCTGGGCT ACTTGATCTG CGGCTTTCGG 1251 GTCGTGCTCA TGTACCGCTT CGAGGAGGAG CTATTCTTGC GCAGCTTGCA 1301 AGACTATAAG ATTCAATCTG CCCTGCTGGT GCCCACACTA TTTAGCTTCT 1351 TCGCTAAGAG CACTCTCATC GACAAGTACG ACCTAAGCAA CTTGCACGAG 1401 ATCGCCAGCG GCGGGGCGCC GCTCAGCAAG GAGGTAGGTG AGGCCGTGGC 1451 CAAACGCTTC CACCTACCAG GCATCCGCCA GGGCTACGGC CTGACAGAAA 1501 CAACCAGCGC CATTCTGATC ACCCCCGAAG GGGACGACAA GCCTGGCGCA 1551 GTAGGCAAGG TGGTGCCCTT CTTCGAGGCT AAGGTGGTGG ACTTGGACAC 1601 CGGTAAGACA CTGGGTGTGA ACCAGCGCGG CGAGCTGTGC GTCCGTGGCC 1651 CCATGATCAT GAGCGGCTAC GTTAACAACC CCGAGGCTAC AAACGCTCTC 1701 ATCGACAAGG ACGGCTGGCT GCACAGCGGC GACATCGCCT ACTGGGACGA 1751 GGACGAGCAC TTCTTCATCG TGGACCGGCT GAAGAGCCTG ATCAAATACA 1801 AGGGCTACCA GGTAGCCCCA GCCGAACTGG AGAGCATCCT GCTGCAACAC 1851 CCCAACATCT TCGACGCCGG GGTCGCCGGC CTGCCCGACG ACGATGCCGG 1901 CGAGCTGCCC GCCGCAGTCG TCGTGCTGGA ACACGGTAAA ACCATGACCG 1951 AGAAGGAGAT CGTGGACTAT GTGGCCAGCC AGGTTACAAC CGCCAAGAAG 2001 CTGCGCGGTG GTGTTGTGTT CGTGGACGAG GTGCCTAAAG GACTGACCGG 2051 CAAGTTGGAC GCCCGCAAGA TCCGCGAGAT TCTCATTAAG GCCAAGAAGG 2101 GCGGCAAGAT CGCCGTGAAT TCTCACGGCT TCCCTCCCGA GGTGGAGGAG 2151 CAGGCCGCCG GCACCCTGCC CATGAGCTGC GCCCAGGAGA GCGGCATGGA 2201 TAGACACCCT GCTGCTTGCG CCAGCGCCAG GATCAACGTC TAAGGCCGCG 2251 ACTCTAGAGT CGGGGCGGCC GGCCGCTTCG AGCAGACATG ATAAGATACA 2301 TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT 2351 ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG 2401 CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC 2451 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT 2501 GGTAAAATCG ATAAGGATCC GTTTGCGTAT TGGGCGCTCT TCCGCTGATC 2551 TGCGCAGCAC CATGGCCTGA AATAACCTCT GAAAGAGGAA CTTGGTTAGC 2601 TACCTTCTGA GGCGGAAAGA ACCAGCTGTG GAATGTGTGT CAGTTAGGGT 2651 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 2701 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG 2751 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC 2801 CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 2851 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC 2901 CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC 2951 TAGGCTTTTG CAAAAAGCTC GATTCTTCTG ACACTAGCGC CACCATGAAG 3001 AAGCCCGAAC TCACCGCTAC CAGCGTTGAA AAATTTCTCA TCGAGAAGTT 3051 CGACAGTGTG AGCGACCTGA TGCAGTTGTC GGAGGGCGAA GAGAGCCGAG 3101 CCTTCAGCTT CGATGTCGGC GGACGCGGCT ATGTACTGCG GGTGAATAGC 3151 TGCGCTGATG GCTTCTACAA AGACCGCTAC GTGTACCGCC ACTTCGCCAG 3201 CGCTGCACTA CCCATCCCCG AAGTGTTGGA CATCGGCGAG TTCAGCGAGA 3251 GCCTGACATA CTGCATCAGT AGACGCGCCC AAGGCGTTAC TCTCCAAGAC 3301 CTCCCCGAAA CAGAGCTGCC TGCTGTGTTA CAGCCTGTCG CCGAAGCTAT 3351 GGATGCTATT GCCGCCGCCG ACCTCAGTCA AACCAGCGGC TTCGGCCCAT 3401 TCGGGCCCCA AGGCATCGGC CAGTACACAA CCTGGCGGGA TTTCATTTGC 3451 GCCATTGCTG ATCCCCATGT CTACCACTGG CAGACCGTGA TGGACGACAC 3501 CGTGTCCGCC AGCGTAGCTC AAGCCCTGGA CGAACTGATG CTGTGGGCCG 3551 AAGACTGTCC CGAGGTGCGC CACCTCGTCC ATGCCGACTT CGGCAGCAAC 3601 AACGTCCTGA CCGACAACGG CCGCATCACC GCCGTAATCG ACTGGTCCGA 3651 AGCTATGTTC GGGGACAGTC AGTACGAGGT GGCCAACATC TTCTTCTGGC 3701 GGCCCTGGCT GGCTTGCATG GAGCAGCAGA CTCGCTACTT CGAGCGCCGG 3751 CATCCCGAGC TGGCCGGCAG CCCTCGTCTG CGAGCCTACA TGCTGCGCAT 3801 CGGCCTGGAT CAGCTCTACC AGAGCCTCGT GGACGGCAAC TTCGACGATG 3851 CTGCCTGGGC TCAAGGCCGC TGCGATGCCA TCGTCCGCAG CGGGGCCGGC 3901 ACCGTCGGTC GCACACAAAT CGCTCGCCGG AGCGCAGCCG TATGGACCGA 3951 CGGCTGCGTC GAGGTGCTGG CCGACAGCGG CAACCGCCGG CCCAGTACAC 4001 GACCGCGCGC TAAGGAGGTA GGTCGAGTTT AAACTCTAGA ACCGGTCATG 4051 GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT TGGTTTTTTG 4101 TGTGTTCGAA CTAGATGCTG TCGACCGATG CCCTTGAGAG CCTTCAACCC 4151 AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC GCCGCACTTA 4201 TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC 4251 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 4301 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA 4351 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 4401 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 4451 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 4501 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 4551 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 4601 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 4651 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 4701 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 4751 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 4801 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 4851 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 4901 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 4951 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 5001 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 5051 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 5101 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 5151 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGC GGCCGCAAAT 5201 GCTAAACCAC TGCAGTGGTT ACCAGTGCTT GATCAGTGAG GCACCGATCT 5251 CAGCGATCTG CCTATTTCGT TCGTCCATAG TGGCCTGACT CCCCGTCGTG 5301 TAGATCACTA CGATTCGTGA GGGCTTACCA TCAGGCCCCA GCGCAGCAAT 5351 GATGCCGCGA GAGCCGCGTT CACCGGCCCC CGATTTGTCA GCAATGAACC 5401 AGCCAGCAGG GAGGGCCGAG CGAAGAAGTG GTCCTGCTAC TTTGTCCGCC 5451 TCCATCCAGT CTATGAGCTG CTGTCGTGAT GCTAGAGTAA GAAGTTCGCC 5501 AGTGAGTAGT TTCCGAAGAG TTGTGGCCAT TGCTACTGGC ATCGTGGTAT 5551 CACGCTCGTC GTTCGGTATG GCTTCGTTCA ACTCTGGTTC CCAGCGGTCA 5601 AGCCGGGTCA CATGATCACC CATATTATGA AGAAATGCAG TCAGCTCCTT 5651 AGGGCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCGGTG TTGTCGCTCA 5701 TGGTAATGGC AGCACTACAC AATTCTCTTA CCGTCATGCC ATCCGTAAGA 5751 TGCTTTTCCG TGACCGGCGA GTACTCAACC AAGTCGTTTT GTGAGTAGTG 5801 TATACGGCGA CCAAGCTGCT CTTGCCCGGC GTCTATACGG GACAACACCG 5851 CGCCACATAG CAGTACTTTG AAAGTGCTCA TCATCGGGAA TCGTTCTTCG 5901 GGGCGGAAAG ACTCAAGGAT CTTGCCGCTA TTGAGATCCA GTTCGATATA 5951 GCCCACTCTT GCACCCAGTT GATCTTCAGC ATCTTTTACT TTCACCAGCG 6001 TTTCGGGGTG TGCAAAAACA GGCAAGCAAA ATGCCGCAAA GAAGGGAATG 6051 AGTGCGACAC GAAAATGTTG GATGCTCAT Sequence file prepared 6-June-2012.