pGL4.37[luc2P/ARE/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGL4.37[luc2P/ARE/Hygro] Vector sequence reference points: Base pairs 6086 Synthetic polyadenylation signal sequence 105-153 Synthetic poly(A) signal/transcriptional pause region 105-258 Reporter vector primer 3 (RVprimer3) binding region 207-226 ARE response element 285-366 Minimal promoter 412-442 luc2P reporter gene 475-2250 SV40 late poly(A) region 2290-2511 SV40 early enhancer/promoter 2559-2977 Synthetic hygromycin (HygR) coding region 3002-4039 Synthetic polyadenylation signal sequence 4063-4111 Reporter vector primer 4 (RVprimer4) binding region 4178-4497 ColE1-derived plasmid replication origin 4435 Synthetic beta-lactamase (AmpR) coding region 5226-6086 .. 1 ACTCGTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TACTAGTACG 51 TCTCTCAAGG ATAAGTAAGT AATATTAAGG TACGGGAGGT ATTGGACAGG 101 CCGCAATAAA ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT 151 GTGAATCGAT AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA 201 AACAAACTAG CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC 251 ATTTCTCTGG CCTAACTGGC CGGTACCTGA GCTCTAGCTT GGAAATGACA 301 TTGCTAATGG TGACAAAGCA ACTTTTAGCT TGGAAATGAC ATTGCTAATG 351 GTGACAAAGC AACTTTCTCG AGGATATCAA GATCTGGCCT CGGCGGCCAA 401 GCTTAGACAC TAGAGGGTAT ATAATGGAAG CTCGACTTCC AGCTTGGCAA 451 TCCGGTACTG TTGGTAAAGC CACCATGGAA GATGCCAAAA ACATTAAGAA 501 GGGCCCAGCG CCATTCTACC CACTCGAAGA CGGGACCGCC GGCGAGCAGC 551 TGCACAAAGC CATGAAGCGC TACGCCCTGG TGCCCGGCAC CATCGCCTTT 601 ACCGACGCAC ATATCGAGGT GGACATTACC TACGCCGAGT ACTTCGAGAT 651 GAGCGTTCGG CTGGCAGAAG CTATGAAGCG CTATGGGCTG AATACAAACC 701 ATCGGATCGT GGTGTGCAGC GAGAATAGCT TGCAGTTCTT CATGCCCGTG 751 TTGGGTGCCC TGTTCATCGG TGTGGCTGTG GCCCCAGCTA ACGACATCTA 801 CAACGAGCGC GAGCTGCTGA ACAGCATGGG CATCAGCCAG CCCACCGTCG 851 TATTCGTGAG CAAGAAAGGG CTGCAAAAGA TCCTCAACGT GCAAAAGAAG 901 CTACCGATCA TACAAAAGAT CATCATCATG GATAGCAAGA CCGACTACCA 951 GGGCTTCCAA AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCGGCT 1001 TCAACGAGTA CGACTTCGTG CCCGAGAGCT TCGACCGGGA CAAAACCATC 1051 GCCCTGATCA TGAACAGTAG TGGCAGTACC GGATTGCCCA AGGGCGTAGC 1101 CCTACCGCAC CGCACCGCTT GTGTCCGATT CAGTCATGCC CGCGACCCCA 1151 TCTTCGGCAA CCAGATCATC CCCGACACCG CTATCCTCAG CGTGGTGCCA 1201 TTTCACCACG GCTTCGGCAT GTTCACCACG CTGGGCTACT TGATCTGCGG 1251 CTTTCGGGTC GTGCTCATGT ACCGCTTCGA GGAGGAGCTA TTCTTGCGCA 1301 GCTTGCAAGA CTATAAGATT CAATCTGCCC TGCTGGTGCC CACACTATTT 1351 AGCTTCTTCG CTAAGAGCAC TCTCATCGAC AAGTACGACC TAAGCAACTT 1401 GCACGAGATC GCCAGCGGCG GGGCGCCGCT CAGCAAGGAG GTAGGTGAGG 1451 CCGTGGCCAA ACGCTTCCAC CTACCAGGCA TCCGCCAGGG CTACGGCCTG 1501 ACAGAAACAA CCAGCGCCAT TCTGATCACC CCCGAAGGGG ACGACAAGCC 1551 TGGCGCAGTA GGCAAGGTGG TGCCCTTCTT CGAGGCTAAG GTGGTGGACT 1601 TGGACACCGG TAAGACACTG GGTGTGAACC AGCGCGGCGA GCTGTGCGTC 1651 CGTGGCCCCA TGATCATGAG CGGCTACGTT AACAACCCCG AGGCTACAAA 1701 CGCTCTCATC GACAAGGACG GCTGGCTGCA CAGCGGCGAC ATCGCCTACT 1751 GGGACGAGGA CGAGCACTTC TTCATCGTGG ACCGGCTGAA GAGCCTGATC 1801 AAATACAAGG GCTACCAGGT AGCCCCAGCC GAACTGGAGA GCATCCTGCT 1851 GCAACACCCC AACATCTTCG ACGCCGGGGT CGCCGGCCTG CCCGACGACG 1901 ATGCCGGCGA GCTGCCCGCC GCAGTCGTCG TGCTGGAACA CGGTAAAACC 1951 ATGACCGAGA AGGAGATCGT GGACTATGTG GCCAGCCAGG TTACAACCGC 2001 CAAGAAGCTG CGCGGTGGTG TTGTGTTCGT GGACGAGGTG CCTAAAGGAC 2051 TGACCGGCAA GTTGGACGCC CGCAAGATCC GCGAGATTCT CATTAAGGCC 2101 AAGAAGGGCG GCAAGATCGC CGTGAATTCT CACGGCTTCC CTCCCGAGGT 2151 GGAGGAGCAG GCCGCCGGCA CCCTGCCCAT GAGCTGCGCC CAGGAGAGCG 2201 GCATGGATAG ACACCCTGCT GCTTGCGCCA GCGCCAGGAT CAACGTCTAA 2251 GGCCGCGACT CTAGAGTCGG GGCGGCCGGC CGCTTCGAGC AGACATGATA 2301 AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA 2351 ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA 2401 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT 2451 CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA 2501 CAAATGTGGT AAAATCGATA AGGATCCGTT TGCGTATTGG GCGCTCTTCC 2551 GCTGATCTGC GCAGCACCAT GGCCTGAAAT AACCTCTGAA AGAGGAACTT 2601 GGTTAGCTAC CTTCTGAGGC GGAAAGAACC AGCTGTGGAA TGTGTGTCAG 2651 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 2701 CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC 2751 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 2801 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC 2851 CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG 2901 AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 2951 GGAGGCCTAG GCTTTTGCAA AAAGCTCGAT TCTTCTGACA CTAGCGCCAC 3001 CATGAAGAAG CCCGAACTCA CCGCTACCAG CGTTGAAAAA TTTCTCATCG 3051 AGAAGTTCGA CAGTGTGAGC GACCTGATGC AGTTGTCGGA GGGCGAAGAG 3101 AGCCGAGCCT TCAGCTTCGA TGTCGGCGGA CGCGGCTATG TACTGCGGGT 3151 GAATAGCTGC GCTGATGGCT TCTACAAAGA CCGCTACGTG TACCGCCACT 3201 TCGCCAGCGC TGCACTACCC ATCCCCGAAG TGTTGGACAT CGGCGAGTTC 3251 AGCGAGAGCC TGACATACTG CATCAGTAGA CGCGCCCAAG GCGTTACTCT 3301 CCAAGACCTC CCCGAAACAG AGCTGCCTGC TGTGTTACAG CCTGTCGCCG 3351 AAGCTATGGA TGCTATTGCC GCCGCCGACC TCAGTCAAAC CAGCGGCTTC 3401 GGCCCATTCG GGCCCCAAGG CATCGGCCAG TACACAACCT GGCGGGATTT 3451 CATTTGCGCC ATTGCTGATC CCCATGTCTA CCACTGGCAG ACCGTGATGG 3501 ACGACACCGT GTCCGCCAGC GTAGCTCAAG CCCTGGACGA ACTGATGCTG 3551 TGGGCCGAAG ACTGTCCCGA GGTGCGCCAC CTCGTCCATG CCGACTTCGG 3601 CAGCAACAAC GTCCTGACCG ACAACGGCCG CATCACCGCC GTAATCGACT 3651 GGTCCGAAGC TATGTTCGGG GACAGTCAGT ACGAGGTGGC CAACATCTTC 3701 TTCTGGCGGC CCTGGCTGGC TTGCATGGAG CAGCAGACTC GCTACTTCGA 3751 GCGCCGGCAT CCCGAGCTGG CCGGCAGCCC TCGTCTGCGA GCCTACATGC 3801 TGCGCATCGG CCTGGATCAG CTCTACCAGA GCCTCGTGGA CGGCAACTTC 3851 GACGATGCTG CCTGGGCTCA AGGCCGCTGC GATGCCATCG TCCGCAGCGG 3901 GGCCGGCACC GTCGGTCGCA CACAAATCGC TCGCCGGAGC GCAGCCGTAT 3951 GGACCGACGG CTGCGTCGAG GTGCTGGCCG ACAGCGGCAA CCGCCGGCCC 4001 AGTACACGAC CGCGCGCTAA GGAGGTAGGT CGAGTTTAAA CTCTAGAACC 4051 GGTCATGGCC GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG 4101 TTTTTTGTGT GTTCGAACTA GATGCTGTCG ACCGATGCCC TTGAGAGCCT 4151 TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC 4201 GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC AGGTGCCGGC 4251 AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 4301 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 4351 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 4401 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 4451 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 4501 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 4551 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 4601 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 4651 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 4701 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 4751 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 4801 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 4851 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT 4901 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 4951 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 5001 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 5051 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 5101 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 5151 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGCGGC 5201 CGCAAATGCT AAACCACTGC AGTGGTTACC AGTGCTTGAT CAGTGAGGCA 5251 CCGATCTCAG CGATCTGCCT ATTTCGTTCG TCCATAGTGG CCTGACTCCC 5301 CGTCGTGTAG ATCACTACGA TTCGTGAGGG CTTACCATCA GGCCCCAGCG 5351 CAGCAATGAT GCCGCGAGAG CCGCGTTCAC CGGCCCCCGA TTTGTCAGCA 5401 ATGAACCAGC CAGCAGGGAG GGCCGAGCGA AGAAGTGGTC CTGCTACTTT 5451 GTCCGCCTCC ATCCAGTCTA TGAGCTGCTG TCGTGATGCT AGAGTAAGAA 5501 GTTCGCCAGT GAGTAGTTTC CGAAGAGTTG TGGCCATTGC TACTGGCATC 5551 GTGGTATCAC GCTCGTCGTT CGGTATGGCT TCGTTCAACT CTGGTTCCCA 5601 GCGGTCAAGC CGGGTCACAT GATCACCCAT ATTATGAAGA AATGCAGTCA 5651 GCTCCTTAGG GCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCGGTGTTG 5701 TCGCTCATGG TAATGGCAGC ACTACACAAT TCTCTTACCG TCATGCCATC 5751 CGTAAGATGC TTTTCCGTGA CCGGCGAGTA CTCAACCAAG TCGTTTTGTG 5801 AGTAGTGTAT ACGGCGACCA AGCTGCTCTT GCCCGGCGTC TATACGGGAC 5851 AACACCGCGC CACATAGCAG TACTTTGAAA GTGCTCATCA TCGGGAATCG 5901 TTCTTCGGGG CGGAAAGACT CAAGGATCTT GCCGCTATTG AGATCCAGTT 5951 CGATATAGCC CACTCTTGCA CCCAGTTGAT CTTCAGCATC TTTTACTTTC 6001 ACCAGCGTTT CGGGGTGTGC AAAAACAGGC AAGCAAAATG CCGCAAAGAA 6051 GGGAATGAGT GCGACACGAA AATGTTGGAT GCTCAT Sequence file prepared 4-June-2012.