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Promoterless Renilla Luciferase Vectors with Puromycin Selection

E6751_pGL4-20-luc2-Puro-Vector_3
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Promoterless Renilla Luciferase Vectors with Puromycin Selection

The pGL4.82[hRluc/Puro], pGL4.83[hRlucP/Puro] and pGL4.84[hRlucCP/Puro] Vectors are designed primarily to accept a putative promoter element for investigation of important regions controlling gene transcription. Alternatively, these vectors may be used as promoterless control vectors in a dual-reporter system with a firefly luciferase vector serving as the experimental vector. The promoterless vectors are available with three varieties of engineered Renilla luciferase genes: hRluc, hRlucP or hRlucCP, and offer a selectable marker, puromycin. The inserts cloned into these vectors can easily be transferred using the multiple cloning site and a unique SfiI transfer scheme.

Applications

  • Transcription regulation.
  • Virus-cell interactions.
  • Compound screening.
  • Post-translational modifications.
  • Promoter analysis.

How the Engineered Renilla Luciferase Genes Work

The hRluc gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization.

About the pGL4 Reporter Vectors

The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Renilla luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.

5140MA-W

pGL4.82[hRluc/Puro] Vector GenBank® Accession Number DQ188846 and vector sequence text file.

5141MA-W

pGL4.83[hRlucP/Puro] Vector GenBank® Accession Number DQ188847 and vector sequence text file.

5142MA-W

pGL4.84[hRlucCP/Puro] Vector GenBank® Accession Number DQ188848 and vector sequence text file.

Specifications

You are viewing: E7501 Change Configuration

What's in the box?

Item Part # Size

pGL4.82[hRluc/Puro] Vector

E750A 1 × 20μg

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: E7511 Change Configuration

What's in the box?

Item Part # Size

pGL4.83[hRlucP/Puro] Vector

E751A 1 × 20μg

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: E7521 Change Configuration

What's in the box?

Item Part # Size

pGL4.84[hRlucCP/Puro] Vector

E752A 1 × 20μg

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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