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Promoterless Renilla Luciferase Vectors with Hygromycin Selection

E6751_pGL4-20-luc2-Puro-Vector_3

We are sorry, but these products are discontinued.

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This product is discontinued
Promoterless Renilla Luciferase Vectors with Hygromycin Selection

The pGL4.76[hRluc/Hygro], pGL4.77[hRlucP/Hygro] and pGL4.78[hRlucCP/Hygro] Vectors are designed primarily to accept a putative promoter element for investigation of important regions controlling gene transcription. Alternatively, these vectors may be used as promoterless control vectors in a dual-reporter system with a firefly luciferase vector serving as the experimental vector. The promoterless vectors are available with three varieties of engineered Renilla luciferase genes: hRluc, hRlucP or hRlucCP, and offer a selectable marker, hygromycin. The inserts cloned into these vectors can easily be transferred using the multiple cloning site and a unique SfiI transfer scheme.

Applications

  • Transcription regulation.
  • Virus-cell interactions.
  • Compound screening.
  • Post-translational modifications.
  • Promoter analysis.

How the Engineered Renilla Luciferase Genes Work

The hRluc gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization. The hRlucP and hRlucCP and RapidResponse™ genes are hRluc genes appended with degradation sequences to influence the cellular half-life of Renilla luciferase. The RapidResponse™ genes respond more rapidly to stimuli but at the expense of signal intensity. The hRlucP gene responds more rapidly than hRluc2 with moderate signal intensity, and the hRlucCP responds more quickly with the lowest signal intensity.

About the pGL4 Reporter Vectors

The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Renilla luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.

4865MA-W

pGL4.76[hRluc/Hygro] Vector GenBank® Accession Number AY864931 and vector sequence text file.

4868MA-W

pGL4.77[hRlucP/Hygro] Vector GenBank® Accession Number AY864932 and vector sequence text file.

4869MA-W

pGL4.78[hRlucCP/Hygro] Vector GenBank® Accession Number AY864933 and vector sequence text file.

Specifications

You are viewing: E6941 Change Configuration

What's in the box?

Item Part # Size

pGL4.76[hRluc/Hygro] Vector

E694A 1 × 20μg

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: E6951 Change Configuration

What's in the box?

Item Part # Size

pGL4.77[hRlucP/Hygro] Vector

E695A 1 × 20μg

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: E6961 Change Configuration

What's in the box?

Item Part # Size

pGL4.78[hRlucCP/Hygro] Vector

E696A 1 × 20μg

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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