This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pNLF1-secN [CMV/Hygro] Vector sequence reference points: Base pairs 6005 CMV immediate early enhancer/promoter 1–742 Chimeric intron 857–989 T7 RNA polymerase promoter 1033–1052 secNanoLuc® protein coding region 1067–1663 Linker 1664–1675 Multiple cloning region 1676–1751 SV40 late poly(A) region 1814–2035 SV40 early enhancer/promoter 2134–2546 Synthetic hygromycin (Hygr) coding region 2577–3614 Synthetic poly(A) signal 3638–3686 β-lactamase (Ampr) coding region 3947–4807 ColE1-derived plasmid replication origin 4962–4998 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCAAA GCCACCATGA ACTCCTTCTC CACAAGCGCC TTCGGTCCAG 1101 TTGCCTTCTC CCTGGGCCTG CTCCTGGTGT TGCCTGCTGC CTTCCCTGCC 1151 CCAGTCTTCA CACTCGAAGA TTTCGTTGGG GACTGGCGAC AGACAGCCGG 1201 CTACAACCTG GACCAAGTCC TTGAACAGGG AGGTGTGTCC AGTTTGTTTC 1251 AGAATCTCGG GGTGTCCGTA ACTCCGATCC AAAGGATTGT CCTGAGCGGT 1301 GAAAATGGGC TGAAGATCGA CATCCATGTC ATCATCCCGT ATGAAGGTCT 1351 GAGCGGCGAC CAAATGGGCC AGATCGAAAA AATTTTTAAG GTGGTGTACC 1401 CTGTGGATGA TCATCACTTT AAGGTGATCC TGCACTATGG CACACTGGTA 1451 ATCGACGGGG TTACGCCGAA CATGATCGAC TATTTCGGAC GGCCGTATGA 1501 AGGCATCGCC GTGTTCGACG GCAAAAAGAT CACTGTAACA GGGACCCTGT 1551 GGAACGGCAA CAAAATTATC GACGAGCGCC TGATCAACCC CGACGGCTCC 1601 CTGCTGTTCC GAGTAACCAT CAACGGAGTG ACCGGCTGGC GGCTGTGCGA 1651 ACGCATTCTG GCGGGCTCGA GCGGCGCGAT CGCTTCCGAA TTCAGAGCTC 1701 AACCGCGGAT ATCTAGATTT GGGCCCAATT CCTGCAGGAT TTTGCGGCCG 1751 CTTGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT 1801 TGGCCGCTTC GAGCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC 1851 CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG 1901 CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC 1951 AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGA TGTGGGAGGT 2001 TTTTTTAAGC AAGTAAAACC TCTACAAATG TGGTAAAATC GAATTTTAAC 2051 AAAATATTAA CGCTTACAAT TTCCTGATGC GGTATTTTCT CCTTACGCAT 2101 CTGTGCGGTA TTTCACACCG CATACGCGGA TCTGCGCAGC ACCATGGCCT 2151 GAAATAACCT CTGAAAGAGG AACTTGGTTA GGTACCTTCT GAGGCGGAAA 2201 GAACCAGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT 2251 CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 2301 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 2351 GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC 2401 CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 2451 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT 2501 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC 2551 TCGATTCTTC TGACACTAGC GCCACCATGA AGAAGCCCGA ACTCACCGCT 2601 ACCAGCGTTG AAAAATTTCT CATCGAGAAG TTCGACAGTG TGAGCGACCT 2651 GATGCAGTTG TCGGAGGGCG AAGAGAGCCG AGCCTTCAGC TTCGATGTCG 2701 GCGGACGCGG CTATGTACTG CGGGTGAATA GCTGCGCTGA TGGCTTCTAC 2751 AAAGACCGCT ACGTGTACCG CCACTTCGCC AGCGCTGCAC TACCCATCCC 2801 CGAAGTGTTG GACATCGGCG AGTTCAGCGA GAGCCTGACA TACTGCATCA 2851 GTAGACGCGC CCAAGGCGTT ACTCTCCAAG ACCTCCCCGA AACAGAGCTG 2901 CCTGCTGTGT TACAGCCTGT CGCCGAAGCT ATGGATGCTA TTGCCGCCGC 2951 CGACCTCAGT CAAACCAGCG GCTTCGGCCC ATTCGGGCCC CAAGGCATCG 3001 GCCAGTACAC AACCTGGCGG GATTTCATTT GCGCCATTGC TGATCCCCAT 3051 GTCTACCACT GGCAGACCGT GATGGACGAC ACCGTGTCCG CCAGCGTAGC 3101 TCAAGCCCTG GACGAACTGA TGCTGTGGGC CGAAGACTGT CCCGAGGTGC 3151 GCCACCTCGT CCATGCCGAC TTCGGCAGCA ACAACGTCCT GACCGACAAC 3201 GGCCGCATCA CCGCCGTAAT CGACTGGTCC GAAGCTATGT TCGGGGACAG 3251 TCAGTACGAG GTGGCCAACA TCTTCTTCTG GCGGCCCTGG CTGGCTTGCA 3301 TGGAGCAGCA GACTCGCTAC TTCGAGCGCC GGCATCCCGA GCTGGCCGGC 3351 AGCCCTCGTC TGCGAGCCTA CATGCTGCGC ATCGGCCTGG ATCAGCTCTA 3401 CCAGAGCCTC GTGGACGGCA ACTTCGACGA TGCTGCCTGG GCTCAAGGCC 3451 GCTGCGATGC CATCGTCCGC AGCGGGGCCG GCACCGTCGG TCGCACACAA 3501 ATCGCTCGCC GGAGCGCAGC CGTATGGACC GACGGCTGCG TCGAGGTGCT 3551 GGCCGACAGC GGCAACCGCC GGCCCAGTAC ACGACCGCGC GCTAAGGAGG 3601 TAGGTCGAGT TTAGACTCTA GCCATCACGA TGGCCGCAAT AAAATATCTT 3651 TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAATC GATAGCGATA 3701 AGGATCCTCT TTGCGCTTGC GTTTTCCCTT GTCCAGATAG CCCAGTAGCT 3751 GACATTCATC CGGGGTCAGC ACCGTTTCTG CGGACTGGCT TTCTACGTAA 3801 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC 3851 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG 3901 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA 3951 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC 4001 CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA 4051 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 4101 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC 4151 ACTTTCAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG 4201 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG 4251 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA 4301 GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT 4351 ACTTCTGACA ACTATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 4401 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT 4451 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC 4501 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC 4551 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT 4601 CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC 4651 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 4701 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG 4751 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA 4801 TTGGTAATTC GAAATGACCG ACCAAGCGAC GCCCAACCGG TATCAGCTCA 4851 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 4901 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC 4951 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 5001 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 5051 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC 5101 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 5151 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 5201 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC 5251 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 5301 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 5351 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT 5401 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 5451 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 5501 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATTT 5551 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 5601 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 5651 CCTAGATCCT TTTATAGTCC GGAAATACAG GAACGCACGC TGGATGGCCC 5701 TTCGCTGGGA TGGTGAAACC ATGAAAAATG GCAGCTTCAG TGGATTAAGT 5751 GGGGGTAATG TGGCCTGTAC CCTCTGGTTG CATAGGTATT CATACGGTTA 5801 AAATTTATCA GGCGCGATTG CGGCAGTTTT TCGGGTGGTT TGTTGCCATT 5851 TTTACCTGTC TGCTGCCGTG ATCGCGCTGA ACGCGTTTTA GCGGTGCGTA 5901 CAATTAAGGG ATTATGGTAA ATCCACTTAC TGTCTGCCCT CGTAGCCATC 5951 GAGATAAACC GCAGTACTCC GGCCACGATG CGTCCGGCGT AGAGGATCGA 6001 GATCT Sequence file created 5-December-2013.