This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN31A Nluc CMV-Hygro Flexi(R) Vector sequence reference points: Base pairs 6308 CMV immediate early enhancer/promoter 1–742 Chimeric intron 857–989 T7 RNA polymerase promoter (–17 to +3) 1033–1052 NanoLuc® protein coding region 1065–1577 Linker 1578–1589 SgfI 1590–1597 Barnase coding region 1621–1956 PmeI 1958–1965 SV40 late poly(A) region 2117–2338 SV40 early enhancer/promoter 2437–2849 Synthetic hygromycin (Hygr) coding region 2880–3917 Synthetic poly(A) signal 3941–3989 β-lactamase (Ampr) coding region 4250–5110 ColE1-derived plasmid replication origin 5265–5301 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGCT CACCATGGTC TTCACACTCG AAGATTTCGT TGGGGACTGG 1101 CGACAGACAG CCGGCTACAA CCTGGACCAA GTCCTTGAAC AGGGAGGTGT 1151 GTCCAGTTTG TTTCAGAATC TCGGGGTGTC CGTAACTCCG ATCCAAAGGA 1201 TTGTCCTGAG CGGTGAAAAT GGGCTGAAGA TCGACATCCA TGTCATCATC 1251 CCGTATGAAG GTCTGAGCGG CGACCAAATG GGCCAGATCG AAAAAATTTT 1301 TAAGGTGGTG TACCCTGTGG ATGATCATCA CTTTAAGGTG ATCCTGCACT 1351 ATGGCACACT GGTAATCGAC GGGGTTACGC CGAACATGAT CGACTATTTC 1401 GGACGGCCGT ATGAAGGCAT CGCCGTGTTC GACGGCAAAA AGATCACTGT 1451 AACAGGGACC CTGTGGAACG GCAACAAAAT TATCGACGAG CGCCTGATCA 1501 ACCCCGACGG CTCCCTGCTG TTCCGAGTAA CCATCAACGG AGTGACCGGC 1551 TGGCGGCTGT GCGAACGCAT TCTGGCGGGC TCGAGCGGCG CGATCGCCAT 1601 GGAATAAGTA AGGAATCCAC ATGGCACAGG TTATCAACAC GTTTGACGGG 1651 GTTGCGGATT ATCTTCAGAC ATATCATAAG CTACCTGATA ATTACATTAC 1701 AAAATCAGAA GCACAAGCCC TCGGCTGGGT GGCATCAAAA GGGAACCTTG 1751 CAGACGTCGC TCCGGGGAAA AGCATCGGCG GAGACATCTT CTCAAACAGG 1801 GAAGGCAAAC TCCCGGGCAA AAGCGGACGA ACATGGCGTG AAGCGGATAT 1851 TAACTATACA TCAGGCTTCA GAAATTCAGA CCGGATTCTT TACTCAAGCG 1901 ACTGGCTGAT TTACAAAACA ACGGACCATT ATCAGACCTT TACAAAAATC 1951 AGATAATGTT TAAACGAATT CGGGCTCGGT ACCCGGGGAT CCTCTAGAGT 2001 CGACCTGCAG GCATGCAAGC TGATCCGGCT GCTAACAAAG CCCGAAAGGA 2051 AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG 2101 GGGCGGCCGC TTCGAGCAGA CATGATAAGA TACATTGATG AGTTTGGACA 2151 AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG 2201 ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC 2251 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGATGTGGGA 2301 GGTTTTTTTA AGCAAGTAAA ACCTCTACAA ATGTGGTAAA ATCGAATTTT 2351 AACAAAATAT TAACGCTTAC AATTTCCTGA TGCGGTATTT TCTCCTTACG 2401 CATCTGTGCG GTATTTCACA CCGCATACGC GGATCTGCGC AGCACCATGG 2451 CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT TCTGAGGCGG 2501 AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG 2551 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 2601 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 2651 CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA 2701 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 2751 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT 2801 ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 2851 AGCTCGATTC TTCTGACACT AGCGCCACCA TGAAGAAGCC CGAACTCACC 2901 GCTACCAGCG TTGAAAAATT TCTCATCGAG AAGTTCGACA GTGTGAGCGA 2951 CCTGATGCAG TTGTCGGAGG GCGAAGAGAG CCGAGCCTTC AGCTTCGATG 3001 TCGGCGGACG CGGCTATGTA CTGCGGGTGA ATAGCTGCGC TGATGGCTTC 3051 TACAAAGACC GCTACGTGTA CCGCCACTTC GCCAGCGCTG CACTACCCAT 3101 CCCCGAAGTG TTGGACATCG GCGAGTTCAG CGAGAGCCTG ACATACTGCA 3151 TCAGTAGACG CGCCCAAGGC GTTACTCTCC AAGACCTCCC CGAAACAGAG 3201 CTGCCTGCTG TGTTACAGCC TGTCGCCGAA GCTATGGATG CTATTGCCGC 3251 CGCCGACCTC AGTCAAACCA GCGGCTTCGG CCCATTCGGG CCCCAAGGCA 3301 TCGGCCAGTA CACAACCTGG CGGGATTTCA TTTGCGCCAT TGCTGATCCC 3351 CATGTCTACC ACTGGCAGAC CGTGATGGAC GACACCGTGT CCGCCAGCGT 3401 AGCTCAAGCC CTGGACGAAC TGATGCTGTG GGCCGAAGAC TGTCCCGAGG 3451 TGCGCCACCT CGTCCATGCC GACTTCGGCA GCAACAACGT CCTGACCGAC 3501 AACGGCCGCA TCACCGCCGT AATCGACTGG TCCGAAGCTA TGTTCGGGGA 3551 CAGTCAGTAC GAGGTGGCCA ACATCTTCTT CTGGCGGCCC TGGCTGGCTT 3601 GCATGGAGCA GCAGACTCGC TACTTCGAGC GCCGGCATCC CGAGCTGGCC 3651 GGCAGCCCTC GTCTGCGAGC CTACATGCTG CGCATCGGCC TGGATCAGCT 3701 CTACCAGAGC CTCGTGGACG GCAACTTCGA CGATGCTGCC TGGGCTCAAG 3751 GCCGCTGCGA TGCCATCGTC CGCAGCGGGG CCGGCACCGT CGGTCGCACA 3801 CAAATCGCTC GCCGGAGCGC AGCCGTATGG ACCGACGGCT GCGTCGAGGT 3851 GCTGGCCGAC AGCGGCAACC GCCGGCCCAG TACACGACCG CGCGCTAAGG 3901 AGGTAGGTCG AGTTTAGACT CTAGCCATCA CGATGGCCGC AATAAAATAT 3951 CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGATAGCG 4001 ATAAGGATCC TCTTTGCGCT TGCGTTTTCC CTTGTCCAGA TAGCCCAGTA 4051 GCTGACATTC ATCCGGGGTC AGCACCGTTT CTGCGGACTG GCTTTCTACG 4101 TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 4151 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT 4201 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 4251 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT 4301 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC 4351 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA 4401 GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 4451 AGCACTTTCA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC 4501 CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 4551 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA 4601 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA 4651 CTTACTTCTG ACAACTATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC 4701 ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 4751 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT 4801 GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 4851 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA 4901 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 4951 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG 5001 GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 5051 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA 5101 GCATTGGTAA TTCGAAATGA CCGACCAAGC GACGCCCAAC CGGTATCAGC 5151 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 5201 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 5251 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 5301 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 5351 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 5401 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 5451 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 5501 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 5551 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 5601 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 5651 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 5701 ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 5751 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 5801 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 5851 TTTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 5901 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 5951 TCACCTAGAT CCTTTTATAG TCCGGAAATA CAGGAACGCA CGCTGGATGG 6001 CCCTTCGCTG GGATGGTGAA ACCATGAAAA ATGGCAGCTT CAGTGGATTA 6051 AGTGGGGGTA ATGTGGCCTG TACCCTCTGG TTGCATAGGT ATTCATACGG 6101 TTAAAATTTA TCAGGCGCGA TTGCGGCAGT TTTTCGGGTG GTTTGTTGCC 6151 ATTTTTACCT GTCTGCTGCC GTGATCGCGC TGAACGCGTT TTAGCGGTGC 6201 GTACAATTAA GGGATTATGG TAAATCCACT TACTGTCTGC CCTCGTAGCC 6251 ATCGAGATAA ACCGCAGTAC TCCGGCCACG ATGCGTCCGG CGTAGAGGAT 6301 CGAGATCT Sequence file created 5-December-2013.