pGL4.28[luc2CP/minP/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGL4.28[luc2CP/minP/Hygro] Vector sequence reference points: Base pairs 6061 Multiple cloning region 1-70 Minimal promoter 78-108 luc2CP reporter gene 141-1970 SV40 late poly(A) region 2007-2228 SV40 early enhancer/promoter 2276-2694 Synthetic hygromycin (Hygr) coding region 2719-3756 Synthetic poly(A) signal 3780-3828 Reporter Vector primer 4 (RVprimer4) binding region 3895-3914 ColE1-derived plasmid replication origin 4152 Synthetic Beta-lactamase (Ampr) coding region 4943-5803 Synthetic poly(A) signal/transcriptional pause site 5908-6061 Reporter Vector primer 3 (RVprimer3) binding region 6010-6029 .. 1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC 51 TGGCCTCGGC GGCCAAGCTT AGACACTAGA GGGTATATAA TGGAAGCTCG 101 ACTTCCAGCT TGGCAATCCG GTACTGTTGG TAAAGCCACC ATGGAAGATG 151 CCAAAAACAT TAAGAAGGGC CCAGCGCCAT TCTACCCACT CGAAGACGGG 201 ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG CCCTGGTGCC 251 CGGCACCATC GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG 301 CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT 351 GGGCTGAATA CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA 401 GTTCTTCATG CCCGTGTTGG GTGCCCTGTT CATCGGTGTG GCTGTGGCCC 451 CAGCTAACGA CATCTACAAC GAGCGCGAGC TGCTGAACAG CATGGGCATC 501 AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC AAAAGATCCT 551 CAACGTGCAA AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA 601 GCAAGACCGA CTACCAGGGC TTCCAAAGCA TGTACACCTT CGTGACTTCC 651 CATTTGCCAC CCGGCTTCAA CGAGTACGAC TTCGTGCCCG AGAGCTTCGA 701 CCGGGACAAA ACCATCGCCC TGATCATGAA CAGTAGTGGC AGTACCGGAT 751 TGCCCAAGGG CGTAGCCCTA CCGCACCGCA CCGCTTGTGT CCGATTCAGT 801 CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG ACACCGCTAT 851 CCTCAGCGTG GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG 901 GCTACTTGAT CTGCGGCTTT CGGGTCGTGC TCATGTACCG CTTCGAGGAG 951 GAGCTATTCT TGCGCAGCTT GCAAGACTAT AAGATTCAAT CTGCCCTGCT 1001 GGTGCCCACA CTATTTAGCT TCTTCGCTAA GAGCACTCTC ATCGACAAGT 1051 ACGACCTAAG CAACTTGCAC GAGATCGCCA GCGGCGGGGC GCCGCTCAGC 1101 AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC CAGGCATCCG 1151 CCAGGGCTAC GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG 1201 AAGGGGACGA CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG 1251 GCTAAGGTGG TGGACTTGGA CACCGGTAAG ACACTGGGTG TGAACCAGCG 1301 CGGCGAGCTG TGCGTCCGTG GCCCCATGAT CATGAGCGGC TACGTTAACA 1351 ACCCCGAGGC TACAAACGCT CTCATCGACA AGGACGGCTG GCTGCACAGC 1401 GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA TCGTGGACCG 1451 GCTGAAGAGC CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC 1501 TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC CGGGGTCGCC 1551 GGCCTGCCCG ACGACGATGC CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT 1601 GGAACACGGT AAAACCATGA CCGAGAAGGA GATCGTGGAC TATGTGGCCA 1651 GCCAGGTTAC AACCGCCAAG AAGCTGCGCG GTGGTGTTGT GTTCGTGGAC 1701 GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA AGATCCGCGA 1751 GATTCTCATT AAGGCCAAGA AGGGCGGCAA GATCGCCGTG AATTCTGCTT 1801 GCAAGAACTG GTTCAGTAGC TTAAGCCACT TTGTGATCCA CCTTAACAGC 1851 CACGGCTTCC CTCCCGAGGT GGAGGAGCAG GCCGCCGGCA CCCTGCCCAT 1901 GAGCTGCGCC CAGGAGAGCG GCATGGATAG ACACCCTGCT GCTTGCGCCA 1951 GCGCCAGGAT CAACGTCTAA CTGCAGTCTA GAGTCGGGGC GGCCGGCCGC 2001 TTCGAGCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT 2051 AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC 2101 TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT 2151 GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA 2201 AGCAAGTAAA ACCTCTACAA ATGTGGTAAA ATCGATAAGG ATCCGTTTGC 2251 GTATTGGGCG CTCTTCCGCT GATCTGCGCA GCACCATGGC CTGAAATAAC 2301 CTCTGAAAGA GGAACTTGGT TAGCTACCTT CTGAGGCGGA AAGAACCAGC 2351 TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 2401 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG 2451 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 2501 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 2551 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT 2601 TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT 2651 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCGATTCT 2701 TCTGACACTA GCGCCACCAT GAAGAAGCCC GAACTCACCG CTACCAGCGT 2751 TGAAAAATTT CTCATCGAGA AGTTCGACAG TGTGAGCGAC CTGATGCAGT 2801 TGTCGGAGGG CGAAGAGAGC CGAGCCTTCA GCTTCGATGT CGGCGGACGC 2851 GGCTATGTAC TGCGGGTGAA TAGCTGCGCT GATGGCTTCT ACAAAGACCG 2901 CTACGTGTAC CGCCACTTCG CCAGCGCTGC ACTACCCATC CCCGAAGTGT 2951 TGGACATCGG CGAGTTCAGC GAGAGCCTGA CATACTGCAT CAGTAGACGC 3001 GCCCAAGGCG TTACTCTCCA AGACCTCCCC GAAACAGAGC TGCCTGCTGT 3051 GTTACAGCCT GTCGCCGAAG CTATGGATGC TATTGCCGCC GCCGACCTCA 3101 GTCAAACCAG CGGCTTCGGC CCATTCGGGC CCCAAGGCAT CGGCCAGTAC 3151 ACAACCTGGC GGGATTTCAT TTGCGCCATT GCTGATCCCC ATGTCTACCA 3201 CTGGCAGACC GTGATGGACG ACACCGTGTC CGCCAGCGTA GCTCAAGCCC 3251 TGGACGAACT GATGCTGTGG GCCGAAGACT GTCCCGAGGT GCGCCACCTC 3301 GTCCATGCCG ACTTCGGCAG CAACAACGTC CTGACCGACA ACGGCCGCAT 3351 CACCGCCGTA ATCGACTGGT CCGAAGCTAT GTTCGGGGAC AGTCAGTACG 3401 AGGTGGCCAA CATCTTCTTC TGGCGGCCCT GGCTGGCTTG CATGGAGCAG 3451 CAGACTCGCT ACTTCGAGCG CCGGCATCCC GAGCTGGCCG GCAGCCCTCG 3501 TCTGCGAGCC TACATGCTGC GCATCGGCCT GGATCAGCTC TACCAGAGCC 3551 TCGTGGACGG CAACTTCGAC GATGCTGCCT GGGCTCAAGG CCGCTGCGAT 3601 GCCATCGTCC GCAGCGGGGC CGGCACCGTC GGTCGCACAC AAATCGCTCG 3651 CCGGAGCGCA GCCGTATGGA CCGACGGCTG CGTCGAGGTG CTGGCCGACA 3701 GCGGCAACCG CCGGCCCAGT ACACGACCGC GCGCTAAGGA GGTAGGTCGA 3751 GTTTAAACTC TAGAACCGGT CATGGCCGCA ATAAAATATC TTTATTTTCA 3801 TTACATCTGT GTGTTGGTTT TTTGTGTGTT CGAACTAGAT GCTGTCGACC 3851 GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG 3901 GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC 3951 GTAGGACAGG TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 4001 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG 4051 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 4101 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 4151 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC 4201 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 4251 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 4301 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 4351 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 4401 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 4451 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG 4501 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 4551 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 4601 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 4651 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 4701 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 4751 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 4801 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 4851 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 4901 CTTGGTCTGA CAGCGGCCGC AAATGCTAAA CCACTGCAGT GGTTACCAGT 4951 GCTTGATCAG TGAGGCACCG ATCTCAGCGA TCTGCCTATT TCGTTCGTCC 5001 ATAGTGGCCT GACTCCCCGT CGTGTAGATC ACTACGATTC GTGAGGGCTT 5051 ACCATCAGGC CCCAGCGCAG CAATGATGCC GCGAGAGCCG CGTTCACCGG 5101 CCCCCGATTT GTCAGCAATG AACCAGCCAG CAGGGAGGGC CGAGCGAAGA 5151 AGTGGTCCTG CTACTTTGTC CGCCTCCATC CAGTCTATGA GCTGCTGTCG 5201 TGATGCTAGA GTAAGAAGTT CGCCAGTGAG TAGTTTCCGA AGAGTTGTGG 5251 CCATTGCTAC TGGCATCGTG GTATCACGCT CGTCGTTCGG TATGGCTTCG 5301 TTCAACTCTG GTTCCCAGCG GTCAAGCCGG GTCACATGAT CACCCATATT 5351 ATGAAGAAAT GCAGTCAGCT CCTTAGGGCC TCCGATCGTT GTCAGAAGTA 5401 AGTTGGCCGC GGTGTTGTCG CTCATGGTAA TGGCAGCACT ACACAATTCT 5451 CTTACCGTCA TGCCATCCGT AAGATGCTTT TCCGTGACCG GCGAGTACTC 5501 AACCAAGTCG TTTTGTGAGT AGTGTATACG GCGACCAAGC TGCTCTTGCC 5551 CGGCGTCTAT ACGGGACAAC ACCGCGCCAC ATAGCAGTAC TTTGAAAGTG 5601 CTCATCATCG GGAATCGTTC TTCGGGGCGG AAAGACTCAA GGATCTTGCC 5651 GCTATTGAGA TCCAGTTCGA TATAGCCCAC TCTTGCACCC AGTTGATCTT 5701 CAGCATCTTT TACTTTCACC AGCGTTTCGG GGTGTGCAAA AACAGGCAAG 5751 CAAAATGCCG CAAAGAAGGG AATGAGTGCG ACACGAAAAT GTTGGATGCT 5801 CATACTCGTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTACTAGT 5851 ACGTCTCTCA AGGATAAGTA AGTAATATTA AGGTACGGGA GGTATTGGAC 5901 AGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT 5951 TGTGTGAATC GATAGTACTA ACATACGCTC TCCATCAAAA CAAAACGAAA 6001 CAAAACAAAC TAGCAAAATA GGCTGTCCCC AGTGCAAGTG CAGGTGCCAG 6051 AACATTTCTC T Sequence file revised 22-Feb-10.