pGL4.27[luc2P/minP/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGL4.27[luc2P/minP/Hygro] Vector sequence reference points: Base pairs 6010 Multiple cloning region 1-70 Minimal promoter 78-108 luc2P reporter gene 141-1916 SV40 late poly(A) region 1956-2177 SV40 early enhancer/promoter 2225-2643 Synthetic hygromycin (Hygr) coding region 2668-3705 Synthetic poly(A) signal 3729-3777 Reporter Vector primer 4 (RVprimer4) binding region 3844-3863 ColE1-derived plasmid replication origin 4101 Synthetic Beta-lactamase (Ampr) coding region 4892-5752 Synthetic poly(A) signal/transcriptional pause site 5857-6010 Reporter Vector primer 3 (RVprimer3) binding region 5959-5978 .. 1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC 51 TGGCCTCGGC GGCCAAGCTT AGACACTAGA GGGTATATAA TGGAAGCTCG 101 ACTTCCAGCT TGGCAATCCG GTACTGTTGG TAAAGCCACC ATGGAAGATG 151 CCAAAAACAT TAAGAAGGGC CCAGCGCCAT TCTACCCACT CGAAGACGGG 201 ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG CCCTGGTGCC 251 CGGCACCATC GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG 301 CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT 351 GGGCTGAATA CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA 401 GTTCTTCATG CCCGTGTTGG GTGCCCTGTT CATCGGTGTG GCTGTGGCCC 451 CAGCTAACGA CATCTACAAC GAGCGCGAGC TGCTGAACAG CATGGGCATC 501 AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC AAAAGATCCT 551 CAACGTGCAA AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA 601 GCAAGACCGA CTACCAGGGC TTCCAAAGCA TGTACACCTT CGTGACTTCC 651 CATTTGCCAC CCGGCTTCAA CGAGTACGAC TTCGTGCCCG AGAGCTTCGA 701 CCGGGACAAA ACCATCGCCC TGATCATGAA CAGTAGTGGC AGTACCGGAT 751 TGCCCAAGGG CGTAGCCCTA CCGCACCGCA CCGCTTGTGT CCGATTCAGT 801 CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG ACACCGCTAT 851 CCTCAGCGTG GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG 901 GCTACTTGAT CTGCGGCTTT CGGGTCGTGC TCATGTACCG CTTCGAGGAG 951 GAGCTATTCT TGCGCAGCTT GCAAGACTAT AAGATTCAAT CTGCCCTGCT 1001 GGTGCCCACA CTATTTAGCT TCTTCGCTAA GAGCACTCTC ATCGACAAGT 1051 ACGACCTAAG CAACTTGCAC GAGATCGCCA GCGGCGGGGC GCCGCTCAGC 1101 AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC CAGGCATCCG 1151 CCAGGGCTAC GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG 1201 AAGGGGACGA CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG 1251 GCTAAGGTGG TGGACTTGGA CACCGGTAAG ACACTGGGTG TGAACCAGCG 1301 CGGCGAGCTG TGCGTCCGTG GCCCCATGAT CATGAGCGGC TACGTTAACA 1351 ACCCCGAGGC TACAAACGCT CTCATCGACA AGGACGGCTG GCTGCACAGC 1401 GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA TCGTGGACCG 1451 GCTGAAGAGC CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC 1501 TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC CGGGGTCGCC 1551 GGCCTGCCCG ACGACGATGC CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT 1601 GGAACACGGT AAAACCATGA CCGAGAAGGA GATCGTGGAC TATGTGGCCA 1651 GCCAGGTTAC AACCGCCAAG AAGCTGCGCG GTGGTGTTGT GTTCGTGGAC 1701 GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA AGATCCGCGA 1751 GATTCTCATT AAGGCCAAGA AGGGCGGCAA GATCGCCGTG AATTCTCACG 1801 GCTTCCCTCC CGAGGTGGAG GAGCAGGCCG CCGGCACCCT GCCCATGAGC 1851 TGCGCCCAGG AGAGCGGCAT GGATAGACAC CCTGCTGCTT GCGCCAGCGC 1901 CAGGATCAAC GTCTAAGGCC GCGACTCTAG AGTCGGGGCG GCCGGCCGCT 1951 TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA 2001 GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT 2051 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG 2101 CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA 2151 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGATAAGGA TCCGTTTGCG 2201 TATTGGGCGC TCTTCCGCTG ATCTGCGCAG CACCATGGCC TGAAATAACC 2251 TCTGAAAGAG GAACTTGGTT AGCTACCTTC TGAGGCGGAA AGAACCAGCT 2301 GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG 2351 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 2401 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 2451 TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA 2501 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 2551 ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA 2601 GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCGATTCTT 2651 CTGACACTAG CGCCACCATG AAGAAGCCCG AACTCACCGC TACCAGCGTT 2701 GAAAAATTTC TCATCGAGAA GTTCGACAGT GTGAGCGACC TGATGCAGTT 2751 GTCGGAGGGC GAAGAGAGCC GAGCCTTCAG CTTCGATGTC GGCGGACGCG 2801 GCTATGTACT GCGGGTGAAT AGCTGCGCTG ATGGCTTCTA CAAAGACCGC 2851 TACGTGTACC GCCACTTCGC CAGCGCTGCA CTACCCATCC CCGAAGTGTT 2901 GGACATCGGC GAGTTCAGCG AGAGCCTGAC ATACTGCATC AGTAGACGCG 2951 CCCAAGGCGT TACTCTCCAA GACCTCCCCG AAACAGAGCT GCCTGCTGTG 3001 TTACAGCCTG TCGCCGAAGC TATGGATGCT ATTGCCGCCG CCGACCTCAG 3051 TCAAACCAGC GGCTTCGGCC CATTCGGGCC CCAAGGCATC GGCCAGTACA 3101 CAACCTGGCG GGATTTCATT TGCGCCATTG CTGATCCCCA TGTCTACCAC 3151 TGGCAGACCG TGATGGACGA CACCGTGTCC GCCAGCGTAG CTCAAGCCCT 3201 GGACGAACTG ATGCTGTGGG CCGAAGACTG TCCCGAGGTG CGCCACCTCG 3251 TCCATGCCGA CTTCGGCAGC AACAACGTCC TGACCGACAA CGGCCGCATC 3301 ACCGCCGTAA TCGACTGGTC CGAAGCTATG TTCGGGGACA GTCAGTACGA 3351 GGTGGCCAAC ATCTTCTTCT GGCGGCCCTG GCTGGCTTGC ATGGAGCAGC 3401 AGACTCGCTA CTTCGAGCGC CGGCATCCCG AGCTGGCCGG CAGCCCTCGT 3451 CTGCGAGCCT ACATGCTGCG CATCGGCCTG GATCAGCTCT ACCAGAGCCT 3501 CGTGGACGGC AACTTCGACG ATGCTGCCTG GGCTCAAGGC CGCTGCGATG 3551 CCATCGTCCG CAGCGGGGCC GGCACCGTCG GTCGCACACA AATCGCTCGC 3601 CGGAGCGCAG CCGTATGGAC CGACGGCTGC GTCGAGGTGC TGGCCGACAG 3651 CGGCAACCGC CGGCCCAGTA CACGACCGCG CGCTAAGGAG GTAGGTCGAG 3701 TTTAAACTCT AGAACCGGTC ATGGCCGCAA TAAAATATCT TTATTTTCAT 3751 TACATCTGTG TGTTGGTTTT TTGTGTGTTC GAACTAGATG CTGTCGACCG 3801 ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG 3851 CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG 3901 TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 3951 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 4001 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 4051 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 4101 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 4151 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 4201 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 4251 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 4301 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 4351 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 4401 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 4451 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 4501 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 4551 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 4601 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 4651 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 4701 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 4751 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 4801 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 4851 TTGGTCTGAC AGCGGCCGCA AATGCTAAAC CACTGCAGTG GTTACCAGTG 4901 CTTGATCAGT GAGGCACCGA TCTCAGCGAT CTGCCTATTT CGTTCGTCCA 4951 TAGTGGCCTG ACTCCCCGTC GTGTAGATCA CTACGATTCG TGAGGGCTTA 5001 CCATCAGGCC CCAGCGCAGC AATGATGCCG CGAGAGCCGC GTTCACCGGC 5051 CCCCGATTTG TCAGCAATGA ACCAGCCAGC AGGGAGGGCC GAGCGAAGAA 5101 GTGGTCCTGC TACTTTGTCC GCCTCCATCC AGTCTATGAG CTGCTGTCGT 5151 GATGCTAGAG TAAGAAGTTC GCCAGTGAGT AGTTTCCGAA GAGTTGTGGC 5201 CATTGCTACT GGCATCGTGG TATCACGCTC GTCGTTCGGT ATGGCTTCGT 5251 TCAACTCTGG TTCCCAGCGG TCAAGCCGGG TCACATGATC ACCCATATTA 5301 TGAAGAAATG CAGTCAGCTC CTTAGGGCCT CCGATCGTTG TCAGAAGTAA 5351 GTTGGCCGCG GTGTTGTCGC TCATGGTAAT GGCAGCACTA CACAATTCTC 5401 TTACCGTCAT GCCATCCGTA AGATGCTTTT CCGTGACCGG CGAGTACTCA 5451 ACCAAGTCGT TTTGTGAGTA GTGTATACGG CGACCAAGCT GCTCTTGCCC 5501 GGCGTCTATA CGGGACAACA CCGCGCCACA TAGCAGTACT TTGAAAGTGC 5551 TCATCATCGG GAATCGTTCT TCGGGGCGGA AAGACTCAAG GATCTTGCCG 5601 CTATTGAGAT CCAGTTCGAT ATAGCCCACT CTTGCACCCA GTTGATCTTC 5651 AGCATCTTTT ACTTTCACCA GCGTTTCGGG GTGTGCAAAA ACAGGCAAGC 5701 AAAATGCCGC AAAGAAGGGA ATGAGTGCGA CACGAAAATG TTGGATGCTC 5751 ATACTCGTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTACTAGTA 5801 CGTCTCTCAA GGATAAGTAA GTAATATTAA GGTACGGGAG GTATTGGACA 5851 GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT 5901 GTGTGAATCG ATAGTACTAA CATACGCTCT CCATCAAAAC AAAACGAAAC 5951 AAAACAAACT AGCAAAATAG GCTGTCCCCA GTGCAAGTGC AGGTGCCAGA 6001 ACATTTCTCT Sequence file prepared 8-Sep-06.