This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pNL3.2.CMV Vector sequence reference points: Base pairs 5591 CMV immediate early enhancer/promoter 14–755 NanoLuc®-PEST protein coding region 859–1497 SV40 late poly(A) region 1537–1758 SV40 early enhancer/promoter 1806–2224 Synthetic hygromycin (Hygr) coding region 2249–3286 ColE1-derived plasmid replication origin 3682–3718 Beta-lactamase (Ampr) coding region 4473–5333 Synthetic poly(A) signal 5438–5486 1 GGCCTAACTG GCCTCAATAT TGGCCATTAG CCATATTATT CATTGGTTAT 51 ATAGCATAAA TCAATATTGG CTATTGGCCA TTGCATACGT TGTATCTATA 101 TCATAATATG TACATTTATA TTGGCTCATG TCCAATATGA CCGCCATGTT 151 GGCATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA 201 GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG 251 CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA 301 CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG 351 GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA 401 TATGCCAAGT CCGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT 451 GGCATTATGC CCAGTACATG ACCTTACGGG ACTTTCCTAC TTGGCAGTAC 501 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA 551 CACCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC 601 ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC 651 TTTCCAAAAT GTCGTAATAA CCCCGCCCCG TTGACGCAAA TGGGCGGTAG 701 GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC 751 AGATCACTAG AAGCTTTATT GCGGTAGTTT ATCACAGTTA AATTGCTAAC 801 GCAGTCAGTG GGCCTCGGCG GCCAAGCTTG GCAATCCGGT ACTGTTGGTA 851 AAGCCACCAT GGTCTTCACA CTCGAAGATT TCGTTGGGGA CTGGCGACAG 901 ACAGCCGGCT ACAACCTGGA CCAAGTCCTT GAACAGGGAG GTGTGTCCAG 951 TTTGTTTCAG AATCTCGGGG TGTCCGTAAC TCCGATCCAA AGGATTGTCC 1001 TGAGCGGTGA AAATGGGCTG AAGATCGACA TCCATGTCAT CATCCCGTAT 1051 GAAGGTCTGA GCGGCGACCA AATGGGCCAG ATCGAAAAAA TTTTTAAGGT 1101 GGTGTACCCT GTGGATGATC ATCACTTTAA GGTGATCCTG CACTATGGCA 1151 CACTGGTAAT CGACGGGGTT ACGCCGAACA TGATCGACTA TTTCGGACGG 1201 CCGTATGAAG GCATCGCCGT GTTCGACGGC AAAAAGATCA CTGTAACAGG 1251 GACCCTGTGG AACGGCAACA AAATTATCGA CGAGCGCCTG ATCAACCCCG 1301 ACGGCTCCCT GCTGTTCCGA GTAACCATCA ACGGAGTGAC CGGCTGGCGG 1351 CTGTGCGAAC GCATTCTGGC GAATTCTCAC GGCTTTCCGC CTGAGGTTGA 1401 AGAGCAAGCC GCCGGTACAT TGCCTATGTC CTGCGCACAA GAAAGCGGTA 1451 TGGACCGGCA CCCAGCCGCT TGTGCTTCAG CTCGCATCAA CGTCTAAGGC 1501 CGCGACTCTA GAGTCGGGGC GGCCGGCCGC TTCGAGCAGA CATGATAAGA 1551 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG 1601 CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA 1651 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG 1701 GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA 1751 ATGTGGTAAA ATCGATAAGG ATCCGTTTGC GTATTGGGCG CTCTTCCGCT 1801 GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT 1851 TAGCTACCTT CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA 1901 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 1951 GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG 2001 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 2051 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 2101 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 2151 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 2201 GGCCTAGGCT TTTGCAAAAA GCTCGATTCT TCTGACACTA GCGCCACCAT 2251 GAAGAAGCCC GAACTCACCG CTACCAGCGT TGAAAAATTT CTCATCGAGA 2301 AGTTCGACAG TGTGAGCGAC CTGATGCAGT TGTCGGAGGG CGAAGAGAGC 2351 CGAGCCTTCA GCTTCGATGT CGGCGGACGC GGCTATGTAC TGCGGGTGAA 2401 TAGCTGCGCT GATGGCTTCT ACAAAGACCG CTACGTGTAC CGCCACTTCG 2451 CCAGCGCTGC ACTACCCATC CCCGAAGTGT TGGACATCGG CGAGTTCAGC 2501 GAGAGCCTGA CATACTGCAT CAGTAGACGC GCCCAAGGCG TTACTCTCCA 2551 AGACCTCCCC GAAACAGAGC TGCCTGCTGT GTTACAGCCT GTCGCCGAAG 2601 CTATGGATGC TATTGCCGCC GCCGACCTCA GTCAAACCAG CGGCTTCGGC 2651 CCATTCGGGC CCCAAGGCAT CGGCCAGTAC ACAACCTGGC GGGATTTCAT 2701 TTGCGCCATT GCTGATCCCC ATGTCTACCA CTGGCAGACC GTGATGGACG 2751 ACACCGTGTC CGCCAGCGTA GCTCAAGCCC TGGACGAACT GATGCTGTGG 2801 GCCGAAGACT GTCCCGAGGT GCGCCACCTC GTCCATGCCG ACTTCGGCAG 2851 CAACAACGTC CTGACCGACA ACGGCCGCAT CACCGCCGTA ATCGACTGGT 2901 CCGAAGCTAT GTTCGGGGAC AGTCAGTACG AGGTGGCCAA CATCTTCTTC 2951 TGGCGGCCCT GGCTGGCTTG CATGGAGCAG CAGACTCGCT ACTTCGAGCG 3001 CCGGCATCCC GAGCTGGCCG GCAGCCCTCG TCTGCGAGCC TACATGCTGC 3051 GCATCGGCCT GGATCAGCTC TACCAGAGCC TCGTGGACGG CAACTTCGAC 3101 GATGCTGCCT GGGCTCAAGG CCGCTGCGAT GCCATCGTCC GCAGCGGGGC 3151 CGGCACCGTC GGTCGCACAC AAATCGCTCG CCGGAGCGCA GCCGTATGGA 3201 CCGACGGCTG CGTCGAGGTG CTGGCCGACA GCGGCAACCG CCGGCCCAGT 3251 ACACGACCGC GCGCTAAGGA GGTAGGTCGA GTTTAAACTC TAGAACCGGT 3301 CATGGCCGCA ATAAAATATC TTTATTTTCA TTACATCTGT GTGTTGGTTT 3351 TTTGTGTGTT CGAACTAGAT GCTGTCGACC GATGCCCTTG AGAGCCTTCA 3401 ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG GCATGACTAT CGTCGCCGCA 3451 CTTATGACTG TCTTCTTTAT CATGCAACTC GTAGGACAGG TGCCGGCAGC 3501 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG 3551 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 3601 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 3651 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 3701 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 3751 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 3801 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT 3851 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 3901 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 3951 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 4001 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 4051 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 4101 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG 4151 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 4201 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA 4251 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG 4301 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 4351 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 4401 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGCGGCCGC 4451 AAATGCTAAA CCACTGCAGT GGTTACCAGT GCTTGATCAG TGAGGCACCG 4501 ATCTCAGCGA TCTGCCTATT TCGTTCGTCC ATAGTGGCCT GACTCCCCGT 4551 CGTGTAGATC ACTACGATTC GTGAGGGCTT ACCATCAGGC CCCAGCGCAG 4601 CAATGATGCC GCGAGAGCCG CGTTCACCGG CCCCCGATTT GTCAGCAATG 4651 AACCAGCCAG CAGGGAGGGC CGAGCGAAGA AGTGGTCCTG CTACTTTGTC 4701 CGCCTCCATC CAGTCTATGA GCTGCTGTCG TGATGCTAGA GTAAGAAGTT 4751 CGCCAGTGAG TAGTTTCCGA AGAGTTGTGG CCATTGCTAC TGGCATCGTG 4801 GTATCACGCT CGTCGTTCGG TATGGCTTCG TTCAACTCTG GTTCCCAGCG 4851 GTCAAGCCGG GTCACATGAT CACCCATATT ATGAAGAAAT GCAGTCAGCT 4901 CCTTAGGGCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC GGTGTTGTCG 4951 CTCATGGTAA TGGCAGCACT ACACAATTCT CTTACCGTCA TGCCATCCGT 5001 AAGATGCTTT TCCGTGACCG GCGAGTACTC AACCAAGTCG TTTTGTGAGT 5051 AGTGTATACG GCGACCAAGC TGCTCTTGCC CGGCGTCTAT ACGGGACAAC 5101 ACCGCGCCAC ATAGCAGTAC TTTGAAAGTG CTCATCATCG GGAATCGTTC 5151 TTCGGGGCGG AAAGACTCAA GGATCTTGCC GCTATTGAGA TCCAGTTCGA 5201 TATAGCCCAC TCTTGCACCC AGTTGATCTT CAGCATCTTT TACTTTCACC 5251 AGCGTTTCGG GGTGTGCAAA AACAGGCAAG CAAAATGCCG CAAAGAAGGG 5301 AATGAGTGCG ACACGAAAAT GTTGGATGCT CATACTCGTC CTTTTTCAAT 5351 ATTATTGAAG CATTTATCAG GGTTACTAGT ACGTCTCTCA AGGATAAGTA 5401 AGTAATATTA AGGTACGGGA GGTATTGGAC AGGCCGCAAT AAAATATCTT 5451 TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAATC GATAGTACTA 5501 ACATACGCTC TCCATCAAAA CAAAACGAAA CAAAACAAAC TAGCAAAATA 5551 GGCTGTCCCC AGTGCAAGTG CAGGTGCCAG AACATTTCTC T Sequence file created 5-December-2013.