This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pNLCoI2[luc2-P2A-NlucP/minP/Hygro] Vector sequence reference points: Base pairs 6589 Multiple cloning region 1–70 Minimal promoter (minP) 78–108 luc2 reporter gene 141–1790 P2A region 1791–1856 NlucP reporter gene 1857–2495 SV40 late poly(A) region 2535–2756 SV40 early enhancer/promoter 2804–3222 Hygromycin resistance coding region 3247–4284 Synthetic poly(A) sequence 4308–4356 Reporter Vector primer 4 (RVprimer4) binding region 4423–4442 Col EI-derived plasmid replication origin 4680 Synthetic β-lactamase (Ampr) coding region 5741–6331 Synthetic poly(A) signal/transcriptional pause region 6436–6589 Reporter Vector primer 3 (RVprimer3) binding region 6538–6557 1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC 51 TGGCCTCGGC GGCCAAGCTT AGACACTAGA GGGTATATAA TGGAAGCTCG 101 ACTTCCAGCT TGGCAATCCG GTACTGTTGG TAAAGCCACC ATGGAAGATG 151 CCAAAAACAT TAAGAAGGGC CCAGCGCCAT TCTACCCACT CGAAGACGGG 201 ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG CCCTGGTGCC 251 CGGCACCATC GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG 301 CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT 351 GGGCTGAATA CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA 401 GTTCTTCATG CCCGTGTTGG GTGCCCTGTT CATCGGTGTG GCTGTGGCCC 451 CAGCTAACGA CATCTACAAC GAGCGCGAGC TGCTGAACAG CATGGGCATC 501 AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC AAAAGATCCT 551 CAACGTGCAA AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA 601 GCAAGACCGA CTACCAGGGC TTCCAAAGCA TGTACACCTT CGTGACTTCC 651 CATTTGCCAC CCGGCTTCAA CGAGTACGAC TTCGTGCCCG AGAGCTTCGA 701 CCGGGACAAA ACCATCGCCC TGATCATGAA CAGTAGTGGC AGTACCGGAT 751 TGCCCAAGGG CGTAGCCCTA CCGCACCGCA CCGCTTGTGT CCGATTCAGT 801 CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG ACACCGCTAT 851 CCTCAGCGTG GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG 901 GCTACTTGAT CTGCGGCTTT CGGGTCGTGC TCATGTACCG CTTCGAGGAG 951 GAGCTATTCT TGCGCAGCTT GCAAGACTAT AAGATTCAAT CTGCCCTGCT 1001 GGTGCCCACA CTATTTAGCT TCTTCGCTAA GAGCACTCTC ATCGACAAGT 1051 ACGACCTAAG CAACTTGCAC GAGATCGCCA GCGGCGGGGC GCCGCTCAGC 1101 AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC CAGGCATCCG 1151 CCAGGGCTAC GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG 1201 AAGGGGACGA CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG 1251 GCTAAGGTGG TGGACTTGGA CACCGGTAAG ACACTGGGTG TGAACCAGCG 1301 CGGCGAGCTG TGCGTCCGTG GCCCCATGAT CATGAGCGGC TACGTTAACA 1351 ACCCCGAGGC TACAAACGCT CTCATCGACA AGGACGGCTG GCTGCACAGC 1401 GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA TCGTGGACCG 1451 GCTGAAGAGC CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC 1501 TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC CGGGGTCGCC 1551 GGCCTGCCCG ACGACGATGC CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT 1601 GGAACACGGT AAAACCATGA CCGAGAAGGA GATCGTGGAC TATGTGGCCA 1651 GCCAGGTTAC AACCGCCAAG AAGCTGCGCG GTGGTGTTGT GTTCGTGGAC 1701 GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA AGATCCGCGA 1751 GATTCTCATT AAGGCCAAGA AGGGCGGCAA GATCGCCGTG GGAAGCGGAG 1801 CTACTAACTT CAGCCTGCTG AAGCAGGCTG GAGACGTGGA GGAGAACCCT 1851 GGACCTATGG TCTTCACACT CGAAGATTTC GTTGGGGACT GGCGACAGAC 1901 AGCCGGCTAC AACCTGGACC AAGTCCTTGA ACAGGGAGGT GTGTCCAGTT 1951 TGTTTCAGAA TCTCGGGGTG TCCGTAACTC CGATCCAAAG GATTGTCCTG 2001 AGCGGTGAAA ATGGGCTGAA GATCGACATC CATGTCATCA TCCCGTATGA 2051 AGGTCTGAGC GGCGACCAAA TGGGCCAGAT CGAAAAAATT TTTAAGGTGG 2101 TGTACCCTGT GGATGATCAT CACTTTAAGG TGATCCTGCA CTATGGCACA 2151 CTGGTAATCG ACGGGGTTAC GCCGAACATG ATCGACTATT TCGGACGGCC 2201 GTATGAAGGC ATCGCCGTGT TCGACGGCAA AAAGATCACT GTAACAGGGA 2251 CCCTGTGGAA CGGCAACAAA ATTATCGACG AGCGCCTGAT CAACCCCGAC 2301 GGCTCCCTGC TGTTCCGAGT AACCATCAAC GGAGTGACCG GCTGGCGGCT 2351 GTGCGAACGC ATTCTGGCGA ATTCTCACGG CTTTCCGCCT GAGGTTGAAG 2401 AGCAAGCCGC CGGTACATTG CCTATGTCCT GCGCACAAGA AAGCGGTATG 2451 GACCGGCACC CAGCCGCTTG TGCTTCAGCT CGCATCAACG TCTAAGGCCG 2501 CGACTCTAGA GTCGGGGCGG CCGGCCGCTT CGAGCAGACA TGATAAGATA 2551 CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT 2601 TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC 2651 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT 2701 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT 2751 GTGGTAAAAT CGATAAGGAT CCGTTTGCGT ATTGGGCGCT CTTCCGCTGA 2801 TCTGCGCAGC ACCATGGCCT GAAATAACCT CTGAAAGAGG AACTTGGTTA 2851 GCTACCTTCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG 2901 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC 2951 ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 3001 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC 3051 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT 3101 CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 3151 GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG 3201 CCTAGGCTTT TGCAAAAAGC TCGATTCTTC TGACACTAGC GCCACCATGA 3251 AGAAGCCCGA ACTCACCGCT ACCAGCGTTG AAAAATTTCT CATCGAGAAG 3301 TTCGACAGTG TGAGCGACCT GATGCAGTTG TCGGAGGGCG AAGAGAGCCG 3351 AGCCTTCAGC TTCGATGTCG GCGGACGCGG CTATGTACTG CGGGTGAATA 3401 GCTGCGCTGA TGGCTTCTAC AAAGACCGCT ACGTGTACCG CCACTTCGCC 3451 AGCGCTGCAC TACCCATCCC CGAAGTGTTG GACATCGGCG AGTTCAGCGA 3501 GAGCCTGACA TACTGCATCA GTAGACGCGC CCAAGGCGTT ACTCTCCAAG 3551 ACCTCCCCGA AACAGAGCTG CCTGCTGTGT TACAGCCTGT CGCCGAAGCT 3601 ATGGATGCTA TTGCCGCCGC CGACCTCAGT CAAACCAGCG GCTTCGGCCC 3651 ATTCGGGCCC CAAGGCATCG GCCAGTACAC AACCTGGCGG GATTTCATTT 3701 GCGCCATTGC TGATCCCCAT GTCTACCACT GGCAGACCGT GATGGACGAC 3751 ACCGTGTCCG CCAGCGTAGC TCAAGCCCTG GACGAACTGA TGCTGTGGGC 3801 CGAAGACTGT CCCGAGGTGC GCCACCTCGT CCATGCCGAC TTCGGCAGCA 3851 ACAACGTCCT GACCGACAAC GGCCGCATCA CCGCCGTAAT CGACTGGTCC 3901 GAAGCTATGT TCGGGGACAG TCAGTACGAG GTGGCCAACA TCTTCTTCTG 3951 GCGGCCCTGG CTGGCTTGCA TGGAGCAGCA GACTCGCTAC TTCGAGCGCC 4001 GGCATCCCGA GCTGGCCGGC AGCCCTCGTC TGCGAGCCTA CATGCTGCGC 4051 ATCGGCCTGG ATCAGCTCTA CCAGAGCCTC GTGGACGGCA ACTTCGACGA 4101 TGCTGCCTGG GCTCAAGGCC GCTGCGATGC CATCGTCCGC AGCGGGGCCG 4151 GCACCGTCGG TCGCACACAA ATCGCTCGCC GGAGCGCAGC CGTATGGACC 4201 GACGGCTGCG TCGAGGTGCT GGCCGACAGC GGCAACCGCC GGCCCAGTAC 4251 ACGACCGCGC GCTAAGGAGG TAGGTCGAGT TTAAACTCTA GAACCGGTCA 4301 TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT 4351 TGTGTGTTCG AACTAGATGC TGTCGACCGA TGCCCTTGAG AGCCTTCAAC 4401 CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT 4451 TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG CCGGCAGCGC 4501 TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 4551 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 4601 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 4651 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 4701 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 4751 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG 4801 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 4851 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 4901 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 4951 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 5001 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 5051 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG 5101 CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 5151 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 5201 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 5251 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 5301 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 5351 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT 5401 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GCGGCCGCAA 5451 ATGCTAAACC ACTGCAGTGG TTACCAGTGC TTGATCAGTG AGGCACCGAT 5501 CTCAGCGATC TGCCTATTTC GTTCGTCCAT AGTGGCCTGA CTCCCCGTCG 5551 TGTAGATCAC TACGATTCGT GAGGGCTTAC CATCAGGCCC CAGCGCAGCA 5601 ATGATGCCGC GAGAGCCGCG TTCACCGGCC CCCGATTTGT CAGCAATGAA 5651 CCAGCCAGCA GGGAGGGCCG AGCGAAGAAG TGGTCCTGCT ACTTTGTCCG 5701 CCTCCATCCA GTCTATGAGC TGCTGTCGTG ATGCTAGAGT AAGAAGTTCG 5751 CCAGTGAGTA GTTTCCGAAG AGTTGTGGCC ATTGCTACTG GCATCGTGGT 5801 ATCACGCTCG TCGTTCGGTA TGGCTTCGTT CAACTCTGGT TCCCAGCGGT 5851 CAAGCCGGGT CACATGATCA CCCATATTAT GAAGAAATGC AGTCAGCTCC 5901 TTAGGGCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCGG TGTTGTCGCT 5951 CATGGTAATG GCAGCACTAC ACAATTCTCT TACCGTCATG CCATCCGTAA 6001 GATGCTTTTC CGTGACCGGC GAGTACTCAA CCAAGTCGTT TTGTGAGTAG 6051 TGTATACGGC GACCAAGCTG CTCTTGCCCG GCGTCTATAC GGGACAACAC 6101 CGCGCCACAT AGCAGTACTT TGAAAGTGCT CATCATCGGG AATCGTTCTT 6151 CGGGGCGGAA AGACTCAAGG ATCTTGCCGC TATTGAGATC CAGTTCGATA 6201 TAGCCCACTC TTGCACCCAG TTGATCTTCA GCATCTTTTA CTTTCACCAG 6251 CGTTTCGGGG TGTGCAAAAA CAGGCAAGCA AAATGCCGCA AAGAAGGGAA 6301 TGAGTGCGAC ACGAAAATGT TGGATGCTCA TACTCGTCCT TTTTCAATAT 6351 TATTGAAGCA TTTATCAGGG TTACTAGTAC GTCTCTCAAG GATAAGTAAG 6401 TAATATTAAG GTACGGGAGG TATTGGACAG GCCGCAATAA AATATCTTTA 6451 TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAATCGA TAGTACTAAC 6501 ATACGCTCTC CATCAAAACA AAACGAAACA AAACAAACTA GCAAAATAGG 6551 CTGTCCCCAG TGCAAGTGCA GGTGCCAGAA CATTTCTCT Sequence file created 17-November-2014.