This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pNLCoI1[luc2-P2A-NlucP/Hygro] Vector sequence reference points: Base pairs 6548 Multiple cloning region 1-70 luc2 reporter gene 100-1749 P2A region 1750-1815 NlucP reporter gene 1816-2454 SV40 late poly(A) region 2494–2715 SV40 early enhancer/promoter 2763–3181 Hygromycin resistance coding region 3206-4243 Synthetic poly(A) sequence 4267-4315 Reporter Vector primer 4 (RVprimer4) binding region 4382-4401 ColE1-derived plasmid replication origin 4639 Synthetic β-lactamase (Ampr) coding region 5430–6290 Synthetic poly(A) signal/transcriptional pause region 6395-6548 Reporter Vector primer 3 (RVprimer3) binding region 6497-6516 1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC 51 TGGCCTCGGC GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA 101 TGGAAGATGC CAAAAACATT AAGAAGGGCC CAGCGCCATT CTACCCACTC 151 GAAGACGGGA CCGCCGGCGA GCAGCTGCAC AAAGCCATGA AGCGCTACGC 201 CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC GAGGTGGACA 251 TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG 301 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA 351 TAGCTTGCAG TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG 401 CTGTGGCCCC AGCTAACGAC ATCTACAACG AGCGCGAGCT GCTGAACAGC 451 ATGGGCATCA GCCAGCCCAC CGTCGTATTC GTGAGCAAGA AAGGGCTGCA 501 AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA AAGATCATCA 551 TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC 601 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA 651 GAGCTTCGAC CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA 701 GTACCGGATT GCCCAAGGGC GTAGCCCTAC CGCACCGCAC CGCTTGTGTC 751 CGATTCAGTC ATGCCCGCGA CCCCATCTTC GGCAACCAGA TCATCCCCGA 801 CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC GGCATGTTCA 851 CCACGCTGGG CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC 901 TTCGAGGAGG AGCTATTCTT GCGCAGCTTG CAAGACTATA AGATTCAATC 951 TGCCCTGCTG GTGCCCACAC TATTTAGCTT CTTCGCTAAG AGCACTCTCA 1001 TCGACAAGTA CGACCTAAGC AACTTGCACG AGATCGCCAG CGGCGGGGCG 1051 CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG GCCAAACGCT TCCACCTACC 1101 AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC GCCATTCTGA 1151 TCACCCCCGA AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC 1201 TTCTTCGAGG CTAAGGTGGT GGACTTGGAC ACCGGTAAGA CACTGGGTGT 1251 GAACCAGCGC GGCGAGCTGT GCGTCCGTGG CCCCATGATC ATGAGCGGCT 1301 ACGTTAACAA CCCCGAGGCT ACAAACGCTC TCATCGACAA GGACGGCTGG 1351 CTGCACAGCG GCGACATCGC CTACTGGGAC GAGGACGAGC ACTTCTTCAT 1401 CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC CAGGTAGCCC 1451 CAGCCGAACT GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC 1501 GGGGTCGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CCGCCGCAGT 1551 CGTCGTGCTG GAACACGGTA AAACCATGAC CGAGAAGGAG ATCGTGGACT 1601 ATGTGGCCAG CCAGGTTACA ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG 1651 TTCGTGGACG AGGTGCCTAA AGGACTGACC GGCAAGTTGG ACGCCCGCAA 1701 GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG ATCGCCGTGG 1751 GAAGCGGAGC TACTAACTTC AGCCTGCTGA AGCAGGCTGG AGACGTGGAG 1801 GAGAACCCTG GACCTATGGT CTTCACACTC GAAGATTTCG TTGGGGACTG 1851 GCGACAGACA GCCGGCTACA ACCTGGACCA AGTCCTTGAA CAGGGAGGTG 1901 TGTCCAGTTT GTTTCAGAAT CTCGGGGTGT CCGTAACTCC GATCCAAAGG 1951 ATTGTCCTGA GCGGTGAAAA TGGGCTGAAG ATCGACATCC ATGTCATCAT 2001 CCCGTATGAA GGTCTGAGCG GCGACCAAAT GGGCCAGATC GAAAAAATTT 2051 TTAAGGTGGT GTACCCTGTG GATGATCATC ACTTTAAGGT GATCCTGCAC 2101 TATGGCACAC TGGTAATCGA CGGGGTTACG CCGAACATGA TCGACTATTT 2151 CGGACGGCCG TATGAAGGCA TCGCCGTGTT CGACGGCAAA AAGATCACTG 2201 TAACAGGGAC CCTGTGGAAC GGCAACAAAA TTATCGACGA GCGCCTGATC 2251 AACCCCGACG GCTCCCTGCT GTTCCGAGTA ACCATCAACG GAGTGACCGG 2301 CTGGCGGCTG TGCGAACGCA TTCTGGCGAA TTCTCACGGC TTTCCGCCTG 2351 AGGTTGAAGA GCAAGCCGCC GGTACATTGC CTATGTCCTG CGCACAAGAA 2401 AGCGGTATGG ACCGGCACCC AGCCGCTTGT GCTTCAGCTC GCATCAACGT 2451 CTAAGGCCGC GACTCTAGAG TCGGGGCGGC CGGCCGCTTC GAGCAGACAT 2501 GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA 2551 AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC 2601 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT 2651 GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC 2701 TCTACAAATG TGGTAAAATC GATAAGGATC CGTTTGCGTA TTGGGCGCTC 2751 TTCCGCTGAT CTGCGCAGCA CCATGGCCTG AAATAACCTC TGAAAGAGGA 2801 ACTTGGTTAG CTACCTTCTG AGGCGGAAAG AACCAGCTGT GGAATGTGTG 2851 TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 2901 AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 2951 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC 3001 CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT 3051 CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 3101 GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT 3151 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CGATTCTTCT GACACTAGCG 3201 CCACCATGAA GAAGCCCGAA CTCACCGCTA CCAGCGTTGA AAAATTTCTC 3251 ATCGAGAAGT TCGACAGTGT GAGCGACCTG ATGCAGTTGT CGGAGGGCGA 3301 AGAGAGCCGA GCCTTCAGCT TCGATGTCGG CGGACGCGGC TATGTACTGC 3351 GGGTGAATAG CTGCGCTGAT GGCTTCTACA AAGACCGCTA CGTGTACCGC 3401 CACTTCGCCA GCGCTGCACT ACCCATCCCC GAAGTGTTGG ACATCGGCGA 3451 GTTCAGCGAG AGCCTGACAT ACTGCATCAG TAGACGCGCC CAAGGCGTTA 3501 CTCTCCAAGA CCTCCCCGAA ACAGAGCTGC CTGCTGTGTT ACAGCCTGTC 3551 GCCGAAGCTA TGGATGCTAT TGCCGCCGCC GACCTCAGTC AAACCAGCGG 3601 CTTCGGCCCA TTCGGGCCCC AAGGCATCGG CCAGTACACA ACCTGGCGGG 3651 ATTTCATTTG CGCCATTGCT GATCCCCATG TCTACCACTG GCAGACCGTG 3701 ATGGACGACA CCGTGTCCGC CAGCGTAGCT CAAGCCCTGG ACGAACTGAT 3751 GCTGTGGGCC GAAGACTGTC CCGAGGTGCG CCACCTCGTC CATGCCGACT 3801 TCGGCAGCAA CAACGTCCTG ACCGACAACG GCCGCATCAC CGCCGTAATC 3851 GACTGGTCCG AAGCTATGTT CGGGGACAGT CAGTACGAGG TGGCCAACAT 3901 CTTCTTCTGG CGGCCCTGGC TGGCTTGCAT GGAGCAGCAG ACTCGCTACT 3951 TCGAGCGCCG GCATCCCGAG CTGGCCGGCA GCCCTCGTCT GCGAGCCTAC 4001 ATGCTGCGCA TCGGCCTGGA TCAGCTCTAC CAGAGCCTCG TGGACGGCAA 4051 CTTCGACGAT GCTGCCTGGG CTCAAGGCCG CTGCGATGCC ATCGTCCGCA 4101 GCGGGGCCGG CACCGTCGGT CGCACACAAA TCGCTCGCCG GAGCGCAGCC 4151 GTATGGACCG ACGGCTGCGT CGAGGTGCTG GCCGACAGCG GCAACCGCCG 4201 GCCCAGTACA CGACCGCGCG CTAAGGAGGT AGGTCGAGTT TAAACTCTAG 4251 AACCGGTCAT GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG 4301 TTGGTTTTTT GTGTGTTCGA ACTAGATGCT GTCGACCGAT GCCCTTGAGA 4351 GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT 4401 CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC 4451 CGGCAGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 4501 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 4551 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG 4601 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 4651 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 4701 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 4751 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 4801 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 4851 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 4901 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 4951 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 5001 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 5051 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 5101 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 5151 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 5201 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 5251 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 5301 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 5351 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 5401 CGGCCGCAAA TGCTAAACCA CTGCAGTGGT TACCAGTGCT TGATCAGTGA 5451 GGCACCGATC TCAGCGATCT GCCTATTTCG TTCGTCCATA GTGGCCTGAC 5501 TCCCCGTCGT GTAGATCACT ACGATTCGTG AGGGCTTACC ATCAGGCCCC 5551 AGCGCAGCAA TGATGCCGCG AGAGCCGCGT TCACCGGCCC CCGATTTGTC 5601 AGCAATGAAC CAGCCAGCAG GGAGGGCCGA GCGAAGAAGT GGTCCTGCTA 5651 CTTTGTCCGC CTCCATCCAG TCTATGAGCT GCTGTCGTGA TGCTAGAGTA 5701 AGAAGTTCGC CAGTGAGTAG TTTCCGAAGA GTTGTGGCCA TTGCTACTGG 5751 CATCGTGGTA TCACGCTCGT CGTTCGGTAT GGCTTCGTTC AACTCTGGTT 5801 CCCAGCGGTC AAGCCGGGTC ACATGATCAC CCATATTATG AAGAAATGCA 5851 GTCAGCTCCT TAGGGCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCGGT 5901 GTTGTCGCTC ATGGTAATGG CAGCACTACA CAATTCTCTT ACCGTCATGC 5951 CATCCGTAAG ATGCTTTTCC GTGACCGGCG AGTACTCAAC CAAGTCGTTT 6001 TGTGAGTAGT GTATACGGCG ACCAAGCTGC TCTTGCCCGG CGTCTATACG 6051 GGACAACACC GCGCCACATA GCAGTACTTT GAAAGTGCTC ATCATCGGGA 6101 ATCGTTCTTC GGGGCGGAAA GACTCAAGGA TCTTGCCGCT ATTGAGATCC 6151 AGTTCGATAT AGCCCACTCT TGCACCCAGT TGATCTTCAG CATCTTTTAC 6201 TTTCACCAGC GTTTCGGGGT GTGCAAAAAC AGGCAAGCAA AATGCCGCAA 6251 AGAAGGGAAT GAGTGCGACA CGAAAATGTT GGATGCTCAT ACTCGTCCTT 6301TTTCAATATT ATTGAAGCAT TTATCAGGGT TACTAGTACG TCTCTCAAGG 6351 ATAAGTAAGT AATATTAAGG TACGGGAGGT ATTGGACAGG CCGCAATAAA 6401 ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGAATCGAT 6451 AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA AACAAACTAG 6501 CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC ATTTCTCT Sequence file created 24-September-2014.