We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Chroma-Luc™ Basic Vectors

Emission spectra of the Chroma-Luc™ enzymes with suggested filter windows.

We are sorry, but these vectors are discontinued.

For more information, contact Technical Service.

Please Enquire
This product is discontinued
Chroma-Luc™ Basic Vectors

The pCBR-Basic, pCBG68-Basic and pCBG99-Basic Chroma-Luc™ Vectors are used for cloning regulatory elements of interest. Cells transfected with Chroma-Luc™ Vectors and used with the Chroma-Glo™ Luciferase Assay System reagent can be used to generate red and green (dual-color) luminescence from a single sample. Use the Control Chroma-Luc™ Vectors serve as an internal control for Basic Vectors.

By having two reporter signals from adding a single substrate, you can increase your accuracy and precision through normalization, or use both reporters to multiplex experimental measurements. Use filters to spectrally separate the luminescent signals. The synthetic gene design of the Chroma-Luc™ Vectors offers high expression with minimal anomalous transcription behavior. The Chroma-Luc™ genes offer ideal control or multiplexed reporter system because the high-homology red and green luciferases minimize potential RNA and protein effects on reporter expression.

Note: Use of this product requires a luminometer fitted with filters. See the Chroma-LucReporter Vectors Technical Manual #TM059 for information on recommended filter sets.

About the Synthetic Click Beetle Luciferase Genes

The Chroma-Luc™ Vectors consist of six plasmids containing synthetic versions of a red or one of two green click beetle luciferase genes; CBRluc contains a red-emitting luciferase gene, while CBG68luc and CBG99luc contain green-emitting luciferase genes. Filtered measurement of the dual-color luminescence produced by the Chroma-Luc™ luciferases permits each reporter to be measured independently and virtually simultaneously. Besides their different luminescence colors, the three Chroma-Luc™ genes differ as follows: CBG99luc and CBRluc possess 99% DNA and 98% protein homology and are the ideal choice for use when working with transient expression assays; CBG68luc and CBRluc possess 68.9% DNA homology while retaining a high degree of protein homology (>98%) and thus are the preferred pair for use with stable expression assays. Each of these genes is provided either in a Basic Vector configuration containing a multiple cloning site (MCS) or a Control Vector containing an SV40 promoter and enhancer. The Chroma-Glo™ Assay has a homogeneous format that generates luminescence with >30-minute signal half-lives for each of the Chroma-Luc™ Luciferases, thereby enabling the processing of many plates without prior sample preparation. Two reporter gene measurements can be efficiently and reproducibly determined from each well in a typical high-throughput screen.

3951MA01_3A-W

pCBR-Basic Vector GenBank® Accession Number AY258591 and vector sequence text file

3956MA01_3A-W

pCBG68-Basic Vector GenBank® Accession Number AY258593 and vector sequence text file.

3953MA01_3A-W

pCBG99-Basic Vector GenBank® Accession Number AY258595 and vector sequence text file.

Specifications

You are viewing: E1411 Change Configuration

What's in the box?

Item Part # SizeConcentration

pCBR-Basic Vector

E141A 1 × 20μg1μg/μl

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Certificate of Analysis

See all certificates for E1411

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: E1431 Change Configuration

What's in the box?

Item Part # SizeConcentration

pCBG68-Basic Vector

E143A 1 × 20μg1μg/μl

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Certificate of Analysis

See all certificates for E1431

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: E1451 Change Configuration

What's in the box?

Item Part # SizeConcentration

pCBG99-Basic Vector

E145A 1 × 20μg1μg/μl

SDS

Choose language:

Download SDSPDF (41 KB) – English (United States)

Certificate of Analysis

See all certificates for E1451

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

Software and Firmware

Let's find the product that meets your needs.

Talk to a Scientist

Jan

Jan

Germany