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New bioluminescent assay to study SARS-CoV-2 neutralizing antibody response. Free webinar.

SARS-CoV-2 Antibody Detection Assay

VB1080_Lumit-Dx-SARS-CoV-2-Immunoassay_3

Simple workflow for detecting antibodies to SARS-CoV-2 in less than one hour in a research setting

  • Fast, no-wash protocol yields reliable results in <1 hour
  • Detects antibodies against the RBD antigen within the spike (S) protein
  • Can be used to study antigen response and seroprevalence in populations
  • Custom assay for research use; please contact us for more information

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Catalog number selected: AX9130

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SARS-CoV-2 Antibody Detection Assay
1 × 96-well plate
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Detect SARS-CoV-2 Antibodies with a Novel Bioluminescent Immunoassay

Lumit™ Immunoassays provide a simple and fast alternative to conventional immunoassay methods including sandwich ELISAs and Western blots. The bioluminescence-based method can be completed in as little as 60 minutes. The Lumit™ SARS-CoV-2 Immunoassay is for research use only.

The Lumit™ SARS-CoV-2 Immunoassay uses a fragment of the spike (S) protein chemically labeled with the small and large subunits of NanoBiT® Luciferase to detect antibodies. Bioluminescent antibody detection offers a simple workflow and lower background signal noise than traditional ELISA antibody tests.

Novel Approach for Antibody Detection

In this Lumit™ Immunoassay, a SARS-CoV-2-specific protein fragment is labeled with the two subunits of the NanoBiT® Luciferase (Lumit™ CoV-2-SmBiT and Lumit™ CoV-2-LgBiT). When these labeled proteins are incubated with a serum or plasma sample that contains SARS-CoV-2 antibodies, the proteins bind to the antibody, bringing the SmBiT and LgBiT subunits in close proximity to form an active luciferase. A bright luminescent signal is generated following the addition of the Lumit™ Detection Reagent and detected using a luminescent-capable microplate reader.

The included calibrator is used to classify samples as positive or negative for SARS-CoV-2 antibodies with a simple calculation.

Lumit™ SARS-CoV-2 Immunoassay detects COVID-19 antibodies with a luminescent signal.

Faster, More Streamlined Workflow Compared to ELISA Assays

Unlike conventional ELISA assays, the Lumit™ SARS-CoV-2 Immunoassay requires no wash steps. This means less hands-on time, and allows you to move through the workflow faster. The assay can go from sample to results in approximately 1 hour. Using a SARS-CoV-2-specific antigen instead of antibodies for detection may offer more robust specificity compared to that of many antibody-based assays.

Typical ELISA Assay Workflow

Conventional COVID-19 ELISA involves multiple wash steps.

Lumit™ SARS-CoV-2 Immunoassay Workflow

Lumit™ SARS-CoV-2 Immunoassay workflow is rapid and scalable.

Specifications

What's in the box?

Item Part # Size

Lumit™ Dx CoV-2 LgBiT

VB104A 1 × 25μl

Lumit™ Dx CoV-2 SmBiT

VB105A 1 × 25μl

Lumit™ Dx Negative Control

VB106A 1 × 200μl

Lumit™ Dx Positive Control

VB107A 1 × 200μl

Lumit™ Dx Assay Calibrator

VB112A 1 × 200μl

Lumit™ Dx Immunoassay Dilution Buffer, 10X

VB113A 1 × 5ml

Lumit™ Dx Detection Substrate

VB114A 1 × 250μl

Lumit™ Dx PBS/EGTA Solution

VB116A 1 × 50ml

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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