SARS-CoV-2 Antibody Detection | COVID Antibody Test Kit

Detect SARS-CoV-2 Antibodies with a Novel Bioluminescent Immunoassay

Lumit™ Immunoassays provide a simple and fast alternative to conventional immunoassay methods including sandwich ELISAs and Western blots. The bioluminescence-based method can be completed in as little as 60 minutes. The Lumit™ SARS-CoV-2 Immunoassay is for research use only.

The Lumit™ SARS-CoV-2 Immunoassay uses a fragment of the spike (S) protein chemically labeled with the small and large subunits of NanoBiT® Luciferase to detect antibodies. Bioluminescent antibody detection offers a simple workflow and lower background signal noise than traditional ELISA antibody tests.

Novel Approach for Antibody Detection

In this Lumit™ Immunoassay, a SARS-CoV-2-specific protein fragment is labeled with the two subunits of the NanoBiT® Luciferase (Lumit™ CoV-2-SmBiT and Lumit™ CoV-2-LgBiT). When these labeled proteins are incubated with a serum or plasma sample that contains SARS-CoV-2 antibodies, the proteins bind to the antibody, bringing the SmBiT and LgBiT subunits in close proximity to form an active luciferase. A bright luminescent signal is generated following the addition of the Lumit™ Detection Reagent and detected using a luminescent-capable microplate reader.

The included calibrator is used to classify samples as positive or negative for SARS-CoV-2 antibodies with a simple calculation.

Lumit™ SARS-CoV-2 Immunoassay detects COVID-19 antibodies with a luminescent signal.

Faster, More Streamlined Workflow Compared to ELISA Assays

Unlike conventional ELISA assays, the Lumit™ SARS-CoV-2 Immunoassay requires no wash steps. This means less hands-on time, and allows you to move through the workflow faster. The assay can go from sample to results in approximately 1 hour. Using a SARS-CoV-2-specific antigen instead of antibodies for detection may offer more robust specificity compared to that of many antibody-based assays.

Typical ELISA Assay Workflow

Conventional COVID-19 ELISA involves multiple wash steps.

Lumit™ SARS-CoV-2 Immunoassay Workflow

What's in the box?

Item	Part #	Size
Lumit™ Dx CoV-2 LgBiT	VB104A	1 × 25μl
Lumit™ Dx CoV-2 SmBiT	VB105A	1 × 25μl
Lumit™ Dx Negative Control	VB106A	1 × 200μl
Lumit™ Dx Positive Control	VB107A	1 × 200μl
Lumit™ Dx Assay Calibrator	VB112A	1 × 200μl
Lumit™ Dx Immunoassay Dilution Buffer, 10X	VB113A	1 × 5ml
Lumit™ Dx Detection Substrate	VB114A	1 × 250μl
Lumit™ Dx PBS/EGTA Solution	VB116A	1 × 50ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

Articles

SARS-CoV-2 and the Antibody Response in COVID-19 Patients

Other Resources

Frequently Used With

GloMax® Navigator System

Dedicated microplate luminometer.

GM2000, GM2010

GloMax® Discover System

High-performance microplate reader for detecting luminescence, fluorescence and absorbance.

GM3000

Promega's Cookie Policy

SARS-CoV-2 Antibody Detection Assay

Detect SARS-CoV-2 Antibodies with a Novel Bioluminescent Immunoassay

Novel Approach for Antibody Detection