The TGFβ1 Emax® ImmunoAssay System provides optimized reagents and a protocol for the sensitive and specific detection of transforming growth factor β1 (TGFβ1). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The system uses horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound TGFβ1. Using this system, biologically active TGFβ1 in tissue culture supernatants, plasma, serum or urine can be quantitated in the range of 15.6–1,000pg/ml.
Note: Plates are not included.