We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

ProtoBlot® II AP Systems with Stabilized Substrate and Western Express Fast Blotting Protocol

We are sorry, but this product is discontinued.

For more information, contact Technical Service.

Introducing the fastest way to order everything you need.

Learn more ›

Please Enquire
This product is discontinued
ProtoBlot® II AP Systems with Stabilized Substrate and Western Express Fast Blotting Protocol

Designed for rapid, sensitive alkaline phosphatase (AP) detection of proteins or other macromolecular antigens immobilized on nitrocellulose or PVDF membranes. Proteins can be transferred from gels after electrophoresis (Western blots) or bound directly from solution ("dot" blots). The Western Express™ Fast Blotting Protocol is included with the system and can reduce dramatically the time required to perform immunodetection, with detection of dot blots in 30–45 minutes and Western blots in 1–2 hours.

The ProtoBlot® II AP Systems contain BSA as a stabilizer and 0.05% sodium azide as a preservative. In addition, the system contains Western Blue® Stabilized Substrate for AP, a ready-to-use solution of BCIP/NBT.

For many applications, AP conjugates are superior to HRP conjugates:

  • Tenfold greater sensitivity of detection
  • Not inhibited by the presence of azide
  • Substrate is not prone to fading during long-term storage
  • Protocols provided for both PVDF and nitrocellulose membranes

References

  1. Towbin, H. et al. (1979) Proc. Natl. Acad. Sci. USA 76, 4350–4.
  2. Burnette, W.N. (1981) Anal. Biochem. 112, 195–203.
  3. Hawkes, R. et al. (1982) Anal. Biochem. 119, 142–7.
  4. Larson, G. (1992) Promega Notes 35, 18–20.
  5. Blake, M.S. et al. (1984) Anal. Biochem. 136, 175–9.

Specifications

You are viewing: W3940 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Anti-Human IgG (H+L) AP Conjugate

S382B 1 × 100μl View Product

Western Blue® Stabilized Substrate for Alkaline Phosphatase

S3841 3 × 100ml

Tween® 20

W383B 1 × 5ml View Product

Blot-Qualified BSA

W384A 1 × 10g View Product

SDS

Choose language:

Download SDSPDF (119 KB) – English (United States)

Certificate of Analysis

See all certificates for W3940

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

Specifications

You are viewing: W3950 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Anti-Mouse IgG (H+L) AP Conjugate

S372B 1 × 100μl View Product

Western Blue® Stabilized Substrate for Alkaline Phosphatase

S3841 3 × 100ml

Tween® 20

W383B 1 × 5ml View Product

Blot-Qualified BSA

W384A 1 × 10g View Product

SDS

Choose language:

Download SDSPDF (119 KB) – English (United States)

Certificate of Analysis

See all certificates for W3950

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

Specifications

You are viewing: W3960 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Anti-Rabbit IgG (Fc) AP Conjugate

S373B 1 × 100μl View Product

Western Blue® Stabilized Substrate for Alkaline Phosphatase

S3841 3 × 100ml

Tween® 20

W383B 1 × 5ml View Product

Blot-Qualified BSA

W384A 1 × 10g View Product

SDS

Choose language:

Download SDSPDF (119 KB) – English (United States)

Certificate of Analysis

See all certificates for W3960

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

 

Let's find the product that meets your needs.

Talk to a Scientist

Paraj

Paraj

US