The GDNF Emax® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of glial cell-line-derived neurotrophic factor (GDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, GDNF in tissue culture supernatants or tissue homogenates can be quantitated in the range of 15.6–1,000pg/ml.
Note: Plates are not included.
- Okragly, A.J. and Haak-Frendscho, M. (1997) Exp. Neurol. 145, 592–6.