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GDNF Emax® ImmunoAssay Systems

Sensitive, Specific Detection of Glial Cell-Line-Derived Neurotrophic Factor in a Sandwich ELISA

  • Specific detection of GDNF; less than 3% cross-reactivity with other related neurotrophic and growth factors
  • Sensitivity to detect picogram levels of factor per milliliter of sample
  • Available kits provide sufficient reagents for two or five 96-well plates; configure your plates as desired

Size

Catalog number selected: G7620

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GDNF Emax® ImmunoAssay Systems
2 × 96 wells
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The GDNF Emax® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of glial cell-line-derived neurotrophic factor (GDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, GDNF in tissue culture supernatants or tissue homogenates can be quantitated in the range of 15.6–1,000pg/ml.

Note: Plates are not included.

Reference

  1. Okragly, A.J. and Haak-Frendscho, M. (1997) Exp. Neurol. 145, 592–6.

Protocols

Specifications

You are viewing: 2 × 96 wells Change Configuration

What's in the box?

Item Part # Size

Anti-Human GDNF pAb

G279C 1 x 20μg

Block & Sample 5X Buffer

G331B 1 x 22ml

Anti-Human GDNF mAb

G332B 1 x 20μl

GDNF Standard

G333B 1 x 20μl

TMB One Solution

G743B 1 x 25ml

Anti-Chicken IgY, HRP

G768A 1 x 80μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Specifications

You are viewing: 5 × 96 wells Change Configuration

What's in the box?

Item Part # Size

Anti-Human GDNF pAb

G279B 1 x 50μg

Block & Sample 5X Buffer

G331A 1 x 54ml

Anti-Human GDNF mAb

G332A 1 x 50μl

GDNF Standard

G333A 1 x 45μl

TMB One Solution

G743B 2 x 25ml

Anti-Chicken IgY, HRP

G768B 1 x 200μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

Resources

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