The BDNF Emax® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of brain-derived neurotrophic factor (BDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use a horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, BDNF in tissue culture supernatants, tissue homogenates, plasma and urine can be quantitated in the range of 7.8–500pg/ml. Binding and recovery from mouse brain homogenates has not been fully characterized.
Note: Plates are not included.