BDNF, a 27kDa homodimer originally derived from human brain, shares high sequence homology with NGF, NT-3 and NT-4/5 and influences many neuron types in the CNS. Anti-Human BDNF pAb is generated in chickens and purified using a proprietary polyethylene glycol procedure. IgY, the 180kDa chicken IgG homolog, can be produced in chickens against certain biological antigens that fail to elicit a humoral immune response in rabbits or other mammals due to species relatedness. This antibody is highly specific for BDNF.
Applications (suggested working concentration)
- Western blotting (1μg/ml).
- ELISA (1μg/ml).
- Immunocytochemistry (1–10μg/ml); methanol-fixed (1–10μg/ml).
- Immunohistochemistry (10–15μg/ml).
- Biological neutralization (10μg/ml).