This antibody [subclass IgG2a(κ)] was purified from ascites of a mouse hybridoma and recognizes E. coli β-galactosidase near the C-terminal end. Antibody is supplied as 2.0–2.5mg/ml in 10mM Tris-HCl (pH 8.0), 150mM NaCl, 0.02% sodium azide.
Applications (suggested working dilution/concentration)
- Immunoprecipitation (25μg/ml).
- Western blotting (1:5,000).
- Dot blot (1:5,000).