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Anti-Human IgG (H+L), AP Conjugate

Secondary Antibody That Binds to Both Heavy and Light Chains for All Human IgG Subclasses

  • Also reacts with light chains on other human immunoglobulins
  • Extensively used and published in numerous research articles
  • Antibody is ready-to-use

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Size

Catalog number selected: S3821

$ 113.00
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This product is discontinued
Anti-Human IgG (H+L), AP Conjugate
100μl
$ 113.00
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Anti-Human IgG (H+L), AP Conjugate

Polyclonal secondary antibody raised in goat, immunoaffinity-purified using immobilized antigens and conjugated to alkaline phosphatase (AP) enzyme. The product (unless otherwise noted) is supplied as a 1mg/ml solution. The Anti-Human IgG (H+L), AP Conjugate antibody reacts with heavy and light chains of all subclasses of human IgG as well as with light chains on other human immunoglobulins; it displays minimal cross-reactivity to horse or bovine serum proteins. As with all antibodies, in certain applications some species-dependent antigen-dependent cross-reactivity may be observed. A starting working dilution of 1:2,500 is suggested for most Western blot, dot blot and ELISA applications. The optimum concentration of secondary antibody depends on the application and will need to be empirically determined.

We can readily accommodate large-scale custom orders. Please inquire at: www.promega.com/custom/

Applications

  • Western blotting.
  • Enzyme-linked immunosorbent assay (ELISA).
  • Dot blotting.

References

  1. Chang, H.-Y., Jordan, J.L. and Krause, D.C. (2011) Domain analysis of protein P30 in Mycoplasma pneumoniae cytadherence and gliding motility. J. Bacteriol. 193, 1726–33.
  2. Prenner, G., Cacho, N.I., Baum, D. and Rudall, P.J. (2011) Is LEAFY a useful marker gene for the flower-inflorescence boundary in the Euphorbia cyathium? J. Exp. Bot. 62, 345–50.
  3. Watanabe, F. and Furuya, E. (2010) Quantitative image analysis reveals that phosphorylation of liver-type isozyme of fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase does not affect nuclear translocation of glucokinase in rat primary hepatocytes. J. Biochem. 148, 713–9.
  4. Wylie, K.M., Schrimpf, J.E. and Morrison, L.A. (2009) Increased eIF2 phosphorylation attenuates replication of herpes simplex virus 2 vhs mutants in mouse embryonic fibroblasts and correlates with reduced accumulation of the PKR antagonist ICP34.5. J. Virol. 83, 9151–62.
  5. Dinglasan, R.R. et al. (2007) Disruption of Plasmodium falciparum development by antibodies against a conserved mosquito midgut antigen. Proc. Natl. Acad. Sci. USA 104, 13461–6.

Specifications

What's in the box?

Item Part # Size

Anti-Human IgG (H+L) AP Conjugate

S382B 1 × 100μl

SDS

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Download SDSPDF (42 KB) – English (United States)

Certificate of Analysis

See all certificates for S3821

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

Resources

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