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EnduRen™ in vivo Renilla Luciferase Substrate is a uniquely engineered coelenterazine-based compound with protected oxidation sites. These modifications are designed to minimize substrate degradation and autoluminescence. EnduRen™ Substrate may have a longer kinetic output compared to the native coelenterazine substrate when used in an in vivo imaging application in a mouse model. Product integrity is ensured by packaging most products in amber vials with septa as well as offering ease of dilution and use for imaging experiments. EnduRen™ Substrate is packaged with fine tolerances to minimize the need to weigh substrates.
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U.S. Pat. Nos. 7,268,229 and 7,537,912 and other patents and patents pending.
Bioluminescence resonance energy transfer (BRET) imaging of protein-protein interactions within deep tissues of living subjects.
Proc. Natl. Acad. Sci. USA
Engineered coelenterazine-based substrate modified to minimize substrate degradation and autoluminescence for animal imaging studies.
Substrate that can be cleaved by the reporter enzyme β-galactosidase and used by firefly luciferase to generate light.
Firefly luciferase substrate capable of generating light for use in animal imaging studies.
P1041, P1042, P1043
Firefly luciferase substrate cleaved by caspase-3 and -7 to generate measurable light.
The pGL4.50 and pGL4.51 Vectors highly express firefly luciferase in stable cell lines for use in animal studies.
Three Renilla vectors with a different promoter (SV40, TK or CMV) that can be used as a control reporter for dual-luciferase assays.
E6911, E6921, E6931
Three Renilla reporter vectors used to clone a putative promoter element for proteins with varying half lives or as a control vector in dual-reporter assays.
E6881, E6891, E6901
Three pGL4 reporter vectors that express Renilla luciferase with varying half lives and have hygromycin resistance.
E6941, E6951, E6961
Three reporter vectors that lack a promoter, have neomycin resistance and code for Renilla luciferase with varying half lives.
E6971, E6981, E6991
Three reporter vectors without a promoter that have puromycin resistance and code for Renilla luciferase with varying half lives.
E7501, E7511, E7521
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