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Genomics Basics: Purifying, Amplifying and Analyzing Nucleic Acids

Seeing Science in Everything We Do.

Being a committed scientific collaborator in your genomics research means that we know that research lives in the everyday moments, and we are there with you. It means seeing science in everything we do. At Promega we understand that you are always engaged, thinking and looking for answers to questions— no matter where you are or what you are doing.

Find a Solution

Whether you are isolating nucleic acid, performing amplifications, cloning, or other molecular biology techniques, we are your partners every step of the way. Download the genomics brochure, and explore our portfolio of molecular biology and genomics products.

Genomics SeeSci Brochure

T-Vector Cloning is the technique that enables direct cloning of PCR products for further analyses.

T-vectors remain some of the most popular vectors sold at Promega, particularly pGEM®-T and pGEM®-T Easy Vector Systems. We have many articles that can support your PCR cloning experiment as well. For tips and tricks, read some of the articles about PCR Cloning and T-Vectors on the Promega Connections blog.

Your cloning tasks run more smoothly if you have everything you need at your fingertips. With the CloneWeaver Workflow Builder, you can gather all of your cloning supplies in on convenient “virtual kit” that matches the needs of your individual cloning experiment. CloneWeaver leads you step by step through the cloning process, from choosing a vector to finding restriction enzymes to getting the ligases and other modifying enzymes you might need. You can save your virtual kit, email it to a purchasing agent, or order it directly from CloneWeaver.

Need to calculate insert to vector ratios to optimize your cloning? Download our Biomath calculator for the web or the mobile app to help you with your calculations.

Our Restriction Enzyme Tool is a convenient mobile app (or web tool) that helps you design those more difficult restriction enzymes. Find the answer to questions like, which buffer will work for two different enzymes? What is the optimal temperature for this digest and more.

Results are key. How are your colleagues using our T-vector cloning products? Peruse the peer-reviewed literature that cites use of pGEM®-T vectors in our citations database and see how others have been successful in their experiments—maybe even pick up a few new ideas for your own work.

We have come a long way from staring at ultra centrifuge tubes held precariously in a ring stand above a UV light box, hoping to capture only the faintly glowing orange band that represented the RNA we desired. Isolating RNA is much easier now, and you have a host of commercially available purification systems. Our RNA and DNA purification product selector can help you find the system that matches your experimental needs and desired throughput.

Even though RNA isolation methods have improved markedly, RNase is still an annoying, ubiquitous contaminant that can refold and regain activity even after autoclaving. Maintaining an RNAase free work zone is still key for success in your RNA-based experiments, but even the most careful application of sterile technique can fall short. RNase Inhibitors like RNasin® Ribonuclease Inhibitor protect RNA from degradation from RNases —value added security, particularly when you are working with rare or limited samples. See how your colleagues have used RNasin® Ribonuclease Inhibitors to protect their work.

There is no replacement for the advice of the post doc down the hall who simply is a genius when it comes to working with RNA, but on those occasions when she’s not around, here is a selection of blogs written about RNA isolation, transcription and other downstream analyses that can help you out when you have questions. There’s even a webinar from one of our own RNA gurus as well.