The PowerQuant® System eliminates guesswork about your DNA sample so that you can make the best decision about what steps to take next. It helps answer questions such as:
- Is this a single-source or mixture sample?
- What amount of DNA should I use in my STR assay?
- Is there sufficient human DNA to proceed with STR analysis?
- Should I perform Y-STR or autosomal STR analysis?
- Is the sample degraded?
- Is the sample inhibited? Should I re-purify?
The PowerQuant® System consistently quantifies as little as 6pg of autosomal or Y-chromosomal DNA and detects <1pg/µl of DNA. If you can't detect DNA with PowerQuant® System, you won't detect it with your STR system. Zero means zero. No need to waste valuable time or STR reagents. Trust your precious DNA samples to the PowerQuant® System, and be confident in your DNA quantitation results.
Use DNA Quantification Results to Reliably Predict STR Results
The PowerQuant® System can detect DNA at concentrations below which an informative profile can be obtained, even when the maximum sample volume is used in an STR amplification reaction.
Casework samples often contain DNA mixtures from multiple persons. The Y-chromosomal target in the PowerQuant® System enables quantification of human male DNA in the sample, helping you evaluate mixtures of male and female DNA.
The PowerQuant® System also allows you to make better decisions when faced with a partial or failed STR amplification by differentiating between PCR inhibition and DNA degradation.
Reliably Detect PCR Inhibitors
Reliable detection of inhibitors that can affect DNA quantification and downstream STR assays is a key feature of the PowerQuant® System. The internal PCR control (IPC) has roughly the same level of sensitivity to inhibitors as currently available downstream STR assays, allowing you to determine if a sample is appropriate for amplification or if additional processing is required.
Assess DNA Integrity of Your DNA Sample
The system amplifies short amplicons that are similar in size to mini-STRs and a long autosomal target. After amplification, the PowerQuant® Analysis tool calculates the ratio of DNA concentrations for the shorter and longer amplicons. The resulting degradation ratio helps you determine if a DNA sample is degraded, rather than inhibited, and decide which, if any, STR system is most appropriate for downstream analysis.