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PowerPlex® S5 System

A0001

Mini-STR Kit for Co-Amplification and Detection of 4 STR Markers

  • 4 STR markers (D18S51, D8S1179, TH01 and FGA) plus Amelogenin
  • 5 loci amplified simultaneously in single tube and analyzed in single injection
  • More tolerant of DNA degradation and less sensitive to inhibitors

Size

Catalog number selected: DC6951

$ 1,624.00 Your price: Log In

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PowerPlex® S5 System
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$ 1,624.00
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Sensitive, Easy to Use STR Kit

The PowerPlex® S5 System is a mini-STR kit that allows co-amplification and detection of four STR markers (D18S51, D8S1179, TH01 and FGA) plus Amelogenin. Full DNA profiles can be achieved with as little as 50pg of DNA and in the presence of 130μM hematin, 200ng tannic acid or 150ng humic acid. The PowerPlex® S5 System is more tolerant of DNA degradation and less sensitive to inhibitors.

This easy to use system comes complete with premixed primer pairs, a master mix with Taq DNA polymerase and internal lane standard. The simplified thermal cycling protocol requires no ramping, and the system is compatible with a number of instrument platforms, including ABI PRISM® 310, 3100 and 3100-Avant and Applied Biosystems® 3130 and 3130xl Genetic Analyzers. Panels and bins text files are required for use with GeneMapper® ID and GeneMapper® ID-X software and are available for download

One primer specific for each of the Amelogenin, D18S51 and D8S1179 loci is labeled with fluorescein (FL), and one primer specific for each of the TH01 and FGA loci is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE). All five loci are amplified simultaneously in a single tube and analyzed in a single injection. The four STR loci are included in the CODIS and European databases. The amplicons for all loci are smaller than 260bp.

The PowerPlex® S5 System is primarily a screening tool but also can be used as a mini-STR casework kit. Use the system to process many samples to exclude known individuals, primarily in casework scenarios to eliminate samples from the victim. You can also screen people in limited geographical areas to eliminate suspects of a crime perpetrated in a town, village or building or screen samples from large-scale catastrophes. The PowerPlex® S5 System can also amplify small STRs, which amplify more efficiently, and capture large alleles that might drop out with megaplex kits.

Sensitivity of the PowerPlex® S5 System

7401TA-W
PowerPlex® S5 profile obtained from 63pg template DNA. One microliter of the amplification was analyzed on an Applied Biosystems 3130xl Genetic Analyzer with a 3kV, 5-second injection.

Specifications

You are viewing: DC6951 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

2800M Control DNA

DD710A 1 × 25μl10ng/μl View Product

PowerPlex® S5 Allelic Ladder Mix

DG100B 1 × 25μl

Internal Lane Standard 600

DG107A 1 × 150μl View Product

PowerPlex® S5 10X Primer Pair Mix

DK111B 1 × 250μl

PowerPlex® S5 5X Master Mix

DP100A 1 × 500μl

Water, Amplification Grade

DW099A 5 × 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

This product is sold under licensing arrangements with Stratagene. The purchase price of this product includes limited, nontransferable rights under U.S. Pat. Nos. 5,449,603, 5,605,824, 5,646,019 and 5,773,257 owned by Stratagene to use only this amount of the product to practice the claims in said patent solely for activities of end users within the fields of life science research and forensic analysis of genetic material relating to, or obtained as the result of, criminal investigations or disaster sites conducted either by or for a governmental entity, or for use in or preparation for legal proceedings, as well as the compilation and indexing of the results of such analysis, and also analysis for parentage determination (the "Forensic and Genetic Identity Applications Field"). The Forensic and Genetic Identity Applications Field specifically excludes tissue typing related to transplantation or other medical procedures. Further licensing information may be obtained by contacting the Business Development Department, Stratagene California, 11011 North Torrey Pines Road, La Jolla, CA 92037.

This product is sold under licensing arrangements with the USB Corporation. The purchase price of this product includes limited, nontransferable rights under U.S. Patent Application Serial Number 11/171,008 owned by the USB Corporation to use only this amount of the product to practice the claims in said patent solely for activities of end users within the fields of life science research and forensic analysis of genetic material relating to, or obtained as the result of, criminal investigations or disaster sites conducted either by or for a governmental entity, or for use in or preparation for legal proceedings, as well as the compilation and indexing of the results of such analysis, and also analysis for parentage determination (the "Forensic and Genetic Identity Applications Field"). The Forensic and Genetic Identity Applications Field specifically excludes tissue typing related to transplantation or other medical procedures. Further licensing information may be obtained by contacting the USB Corporation, 26111 Miles Road, Cleveland, OH 44128.

Specifications

You are viewing: DC6950 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

2800M Control DNA

DD710A 1 × 25μl10ng/μl View Product

PowerPlex® S5 Allelic Ladder Mix

DG100B 4 × 25μl

Internal Lane Standard 600

DG107A 4 × 150μl View Product

PowerPlex® S5 10X Primer Pair Mix

DK111B 4 × 250μl

PowerPlex® S5 5X Master Mix

DP100A 4 × 500μl

Water, Amplification Grade

DW099A 10 × 1,250μl View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

This product is sold under licensing arrangements with Stratagene. The purchase price of this product includes limited, nontransferable rights under U.S. Pat. Nos. 5,449,603, 5,605,824, 5,646,019 and 5,773,257 owned by Stratagene to use only this amount of the product to practice the claims in said patent solely for activities of end users within the fields of life science research and forensic analysis of genetic material relating to, or obtained as the result of, criminal investigations or disaster sites conducted either by or for a governmental entity, or for use in or preparation for legal proceedings, as well as the compilation and indexing of the results of such analysis, and also analysis for parentage determination (the "Forensic and Genetic Identity Applications Field"). The Forensic and Genetic Identity Applications Field specifically excludes tissue typing related to transplantation or other medical procedures. Further licensing information may be obtained by contacting the Business Development Department, Stratagene California, 11011 North Torrey Pines Road, La Jolla, CA 92037.

This product is sold under licensing arrangements with the USB Corporation. The purchase price of this product includes limited, nontransferable rights under U.S. Patent Application Serial Number 11/171,008 owned by the USB Corporation to use only this amount of the product to practice the claims in said patent solely for activities of end users within the fields of life science research and forensic analysis of genetic material relating to, or obtained as the result of, criminal investigations or disaster sites conducted either by or for a governmental entity, or for use in or preparation for legal proceedings, as well as the compilation and indexing of the results of such analysis, and also analysis for parentage determination (the "Forensic and Genetic Identity Applications Field"). The Forensic and Genetic Identity Applications Field specifically excludes tissue typing related to transplantation or other medical procedures. Further licensing information may be obtained by contacting the USB Corporation, 26111 Miles Road, Cleveland, OH 44128.

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