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PowerPlex® ESX 17 and ESI 17 Fast Systems

Co-Amplification and 5-Color Detection of ENFSI-Recommended STR Loci

  • Amplification and detection in <50 minutes
  • Multiple kit configurations help confirm results from poor-quality samples
  • These systems include SE33 locus

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Catalog number selected: DC1710

$ 9,607.00
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PowerPlex® ESX 17 and ESI 17 Fast Systems
ESX/400 reactions
$ 9,607.00
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Achieve Superior Results in Less Time

The PowerPlex® ESX 17 and ESI 17 Fast Systems are designed for human identification applications including forensic analysis, relationship testing and research use. These kits can be used for both casework and database samples, simplifying workflows. These systems meet the ENFSI recommendations for DNA profile sharing across Europe and allow co-amplification and detection of D3S1358, D8S1179, D18S51, D21S11, FGA, TH01, vWA, D2S441, D10S1248, D22S1045, D1S1656, D12S391, D2S1338, D16S539, D19S433, SE33 and Amelogenin.

These systems provide all materials necessary to amplify STR regions of human genomic DNA, including a hot-start thermostable DNA polymerase. Rapid cycling technology enables amplification to be done in less than 50 minutes. Additionally, the kits have superior tolerance to common inhibitors and superior sensitivity to obtain full profiles from low-level DNA and are robust enough to genotype degraded DNA samples through the use of miniSTR loci.

These two STR systems can be used to complement each other when analyzing degraded or challenging samples to maximize recovery of allelic information from as many loci as possible and allow confirmation of results obtained with the other system.

This system is compatible with ABI PRISM® 310, 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.

ForensicGrade

Promega forensic products are manufactured in alignment with the ISO 18385 standard.This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.

PowerPlex® ESX 17 Fast System

The PowerPlex® ESX 17 Fast System amplifies the loci recommended by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP) as mini-STRs (<125bp; D2S441, D10S1248 and D22S1045) or midi-STRs (125–185bp; D1S1656 and D12S391).

13658TA-W-2
2800M Control DNA (0.5ng) was amplified with the PowerPlex® ESX 17 Fast System for 30 cycles. Amplification products were separated using an Applied Biosystems® 3130 Genetic Analyzer (3kV, 5-second injection).

PowerPlex® ESI 17 Fast System

The PowerPlex® ESI 17 Fast System is designed with six of the original seven European Standard Set (ESS) loci (D3S1358, D18S51, TH01, vWA, D8S1179 and the more common FGA alleles) along with D16S539 and D19S433 as smaller amplicons (<250bp), while the loci recommended by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP) (D1S1656, D2S441, D10S1248, D12S391 and D22S1045) are present as larger amplicons.

13660TA-W
2800M Control DNA (0.5ng) was amplified with the PowerPlex® ESI 17 Fast System for 30 cycles. Amplification products were separated using an Applied Biosystems® 3130 Genetic Analyzer (3kV, 5-second injection).

Improved Sensitivity for More Meaningful Analyses

The PowerPlex® ESI and ESX Fast Systems reliably produce complete STR profiles from as little as 100pg of human DNA, and the majority of alleles can be called with as little as 30pg of DNA.

DNA Amount PowerPlex® ESI 17 Fast System PowerPlex® ESI 17 Pro System PowerPlex® ESX 17 Fast System PowerPlex® ESX 17 System
500pg 100% 100% 100% 100%
100pg 100% 100% 100% 100%
60pg 100% 99% 100% 100%
30pg 85% 76% 98% 90%

Perform Direct Amplification from a Variety of Sample Types

The PowerPlex® ESI and ESX Fast Systems allow direct amplification from FTA® card punches as well as pretreated nonFTA cards and commonly used swabs to streamline workflow and expand sample throughput.

Blood Sample on FTA® Card

11517MA-W
Direct amplification of one 1.2mm punch from a blood sample on an FTA® card using the PowerPlex® ESI 17 Fast System and the recommended protocol. Amplified products were separated using an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).

Buccal Sample on FTA® Card

11518MA-W
Direct amplification of two 1.2mm punches from a buccal sample on an FTA® card using the PowerPlex® ESI 17 Fast System and the recommended protocol. Amplified products were separated using an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).

Buccal Swab with SwabSolution™ Reagent

11519MA-W
A buccal swab was pretreated with SwabSolution™ Reagent. Following incubation, 2µl of extract was added to the PCR amplification mix and amplified using the PowerPlex® ESI 17 Fast System and the recommended protocol. Amplified products were separated using an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).

Buccal Sample with PunchSolution™ Reagent

11520MA-W
A single 1.2mm punch from a buccal sample collected using a Bode Buccal DNA Collector™ device was pretreated with PunchSolution™ reagent, then amplified using the PowerPlex® ESI 17 Fast System and the recommended protocol. Amplified products were separated using an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).

Compare with NGM Detect™ and SElect™ Kits

PowerPlex 17 Fast Comparison with NGM Detect and Select Kits

Rapid Processing Solutions for PowerPlex®

The 5X AmpSolution™ Reagent, SwabSolution™ Kit and PunchSolution™ Kit allow fast and simple processing of swabs and punches for analysis with various PowerPlex® Systems, including the PowerPlex® Fusion, 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems. These products are intended for preparation of single-source reference, database and paternity samples where sample purification is unnecessary.

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Which Product is Right for Me?

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Want to Automate?

Want to spend more time on STR data analysis? Let the STR Normalization Manager Software set up your PCR amplifications using the Biomek® 4000 (Beckman Coulter), Freedom EVO (Tecan) or MicroLab STAR (Hamilton) liquid handlers. The software uses quantitation data from the PowerQuant® or Plexor® HY Systems to accurately dilute and set up STR amplification of extracted DNA. The software can also set up direct amplification from swabs and card punches.

Automate Your STR Setup

Contact your local representative to receive a quote for the required quantities and to purchase and schedule the required installation and training services.

Consumables
Product Size Cat. #
1.1ml, Square-Well V-bottom Deep Well Plate
25/pack
2.2ml, Square-Well Deep Well Plate
50/pack
Water, Amplification Grade
6,250ml
Hardware
Product Cat. #
Four-Position Tube Holder
Heat Block Adapter
Plate Clamp 96
Plate Stand
Software
Product Cat. #
STR Normalization Manager Software

Specifications

You are viewing: DC1710 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

PowerPlex® ESI/ESX Fast 5X Master Mix

DP113A 4 × 500μl

PowerPlex® ESX 17 Fast 10X Primer Pair Mix

DK245B 4 × 250μl

2800M Control DNA

DD710A 1 × 25μl10ng/μl View Product

Water, Amplification Grade

DW099A 10 × 1,250μl View Product

PowerPlex® ESX 17 Fast Allelic Ladder Mix

DG385B 4 × 50μl

WEN Internal Lane Standard 500 ESS

DG510A 2 × 200μl View Product

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229 and other patents pending.

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Australian Pat. No. 724531, Canadian Pat. No. 2,251,793 and other patents and patents pending.

Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

TMR-ET, CXR-ET and WEN dyes are proprietary.

Specifications

You are viewing: DC1711 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

PowerPlex® ESI/ESX Fast 5X Master Mix

DP113A 1 × 500μl

PowerPlex® ESX 17 Fast 10X Primer Pair Mix

DK245B 1 × 250μl

2800M Control DNA

DD710A 1 × 25μl10ng/μl View Product

Water, Amplification Grade

DW099A 5 × 1,250μl View Product

PowerPlex® ESX 17 Fast Allelic Ladder Mix

DG385B 1 × 50μl

WEN Internal Lane Standard 500 ESS

DG510A 1 × 200μl View Product

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for DC1711

Search

View more results

No results

Loading…

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229 and other patents pending.

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Australian Pat. No. 724531, Canadian Pat. No. 2,251,793 and other patents and patents pending.

Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

TMR-ET, CXR-ET and WEN dyes are proprietary.

Specifications

You are viewing: DC1720 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

PowerPlex® ESI/ESX Fast 5X Master Mix

DP113A 4 × 500μl

PowerPlex® ESI 17 Fast 10X Primer Pair Mix

DK244B 4 × 250μl

2800M Control DNA

DD710A 1 × 25μl10ng/μl View Product

Water, Amplification Grade

DW099A 10 × 1,250μl View Product

PowerPlex® ESI 17 Fast Allelic Ladder Mix

DG386B 4 × 50μl

WEN Internal Lane Standard 500 ESS

DG510A 2 × 200μl View Product

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for DC1720

Search

View more results

No results

Loading…

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229 and other patents pending.

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Australian Pat. No. 724531, Canadian Pat. No. 2,251,793 and other patents and patents pending.

Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

TMR-ET, CXR-ET and WEN dyes are proprietary.

Specifications

You are viewing: DC1721 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

PowerPlex® ESI/ESX Fast 5X Master Mix

DP113A 1 × 500μl

PowerPlex® ESI 17 Fast 10X Primer Pair Mix

DK244B 1 × 250μl

2800M Control DNA

DD710A 1 × 25μl10ng/μl View Product

Water, Amplification Grade

DW099A 5 × 1,250μl View Product

PowerPlex® ESI 17 Fast Allelic Ladder Mix

DG386B 1 × 50μl

WEN Internal Lane Standard 500 ESS

DG510A 1 × 200μl View Product

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for DC1721

Search

View more results

No results

Loading…

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229 and other patents pending.

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Australian Pat. No. 724531, Canadian Pat. No. 2,251,793 and other patents and patents pending.

Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

TMR-ET, CXR-ET and WEN dyes are proprietary.

Specifications

You are viewing: DC1731 Change Configuration

What's in the box?

Item Part # Size

PowerPlex® ESX 17 Fast System

DC1711 1 × 100 reactions

PowerPlex® ESI 17 Fast System

DC1721 1 × 100 reactions

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for DC1731

Search

View more results

No results

Loading…

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229 and other patents pending.

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Australian Pat. No. 724531, Canadian Pat. No. 2,251,793 and other patents and patents pending.

Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

TMR-ET, CXR-ET and WEN dyes are proprietary.

Specifications

You are viewing: DC1730 Change Configuration

What's in the box?

Item Part # Size

PowerPlex® ESX 17 Fast System

DC1710 1 × 400 reactions

PowerPlex® ESI 17 Fast System

DC1720 1 × 400 reactions

SDS

Choose language:

Download SDS PDF (0 B)

Certificate of Analysis

See all certificates for DC1730

Search

View more results

No results

Loading…

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 9,139,868, European Pat. No. 2972229 and other patents pending.

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Australian Pat. No. 724531, Canadian Pat. No. 2,251,793 and other patents and patents pending.

Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.

TMR-ET, CXR-ET and WEN dyes are proprietary.

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