The PowerPlex® 16 and ES Monoplex Systems are available to amplify the Penta E, Penta D, SE33, D3S1358, TH01, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, CSF1PO, vWA, D8S1179, TPOX or FGA locus. Each monoplex system allows amplification of a single locus to confirm results obtained with the PowerPlex® 16 or discontinued PowerPlex® ES System. The monoplex systems also can be used to re-amplify DNA samples when one or more of the loci do not amplify initially due to suboptimal amplification conditions or poor DNA quality.
The PowerPlex® 16 and PowerPlex® ES Monoplex Systems contain primer pairs that have the same sequence as those used in the PowerPlex® 16 HS (Cat.# DC2100, DC2101), PowerPlex® 16 (Cat.# DC6530, DC6531), PowerPlex® 16 BIO (Cat.# DC6540) and discontinued PowerPlex® ES Systems (Cat.# DC6730, DC6731).
Allelic ladders are only provided in the following PowerPlex® Monoplex Systems: DC6751, DC6591 and DC6651 [SE33 (JOE); Penta E (fluorescein) and Penta D (JOE), respectively].
Allelic ladders that are not provided are available by custom order. Contact Technical Services for allelic ladder options based on the platform used.
The PowerPlex® 16 and ES Monoplex Systems were developed for use with the ABI PRISM® 310, 3100 and 3100-Avant and Applied Biosystems® 3130 and 3130xl Genetic Analyzers and are compatible with the Hitachi FMBIO® II Fluorescence Imaging System.