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PowerQuant® System

pq5002o-W

5-Color, 4-Target Probe-Based qPCR Assay

  • Quantifies total amount of amplifiable autosomal and Y-chromosomal DNA in single assay
  • Consistent and reproducible detection of 6pg of DNA
  • Detects PCR inhibitors and assesses DNA integrity of your sample

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Catalog number selected: PQ5002

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PowerQuant® System
PowerQuant® System/200 reactions
$ 928.00
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Accurate and Sensitive DNA Quantification on the First Try

The PowerQuant® System is a 5-color, 4-target probe-based qPCR assay that quantifies the total amount of amplifiable autosomal and Y-chromosomal DNA in a single assay using the same DNA standards. This system provides flexible options for 4-point to 7-point standard curve. The kit contains an internal PCR control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors.

  • Trust your zero quantification results—zero means zero
  • Obtain reliable quantification results with more consistent auto/Y ratios
  • Maximize your success in STR assays with straightforward DNA quality assessment
  • Shorten time to results with a faster cycling time of approximately 1 hour

 The PowerQuant® System is optimized for use on the Applied Biosystems® 7500 Real-Time PCR System (Applied Biosystems® 7500 Software, Version 2.0.6) or Applied Biosystems® 7500 Real-Time PCR System for Human Identification (HID Real-Time PCR Analysis, Version 1.1 or 1.2).

ForensicGrade

Promega forensic products are manufactured in alignment with the ISO 18385 standard.This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.

The Power of Sensitivity

The PowerQuant® System consistently quantifies as little as 6pg of autosomal or Y-chromosomal DNA and detects <1pg/μl of DNA. If you can't detect DNA with PowerQuant® System, you won't detect it with your STR system, no need to waste valuable time or STR reagents. The PowerQuant® System eliminates guesswork about your DNA sample so that you can make the best decision about what steps to take next and answer questions such as:

  • Is this a single-source or mixture sample?
  • What amount of DNA should I use in my STR assay?
  • Is there sufficient human DNA to proceed with STR analysis?
  • Should I perform Y-STR or autosomal STR analysis?
  • Is the sample degraded?
  • Is the sample inhibited? Should I re-purify?
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A DNA sample that had no Cq value for the autosomal, Y and degradation targets was amplified using the PowerPlex® ESI 17 System. Even when the maximum volume of DNA sample (17.5μl) was added to the STR amplification, no STR profile was generated.

Use DNA Quantification Results to Reliably Predict STR Results

The PowerQuant® System can detect DNA at concentrations below which an informative profile can be obtained, even when the maximum sample volume is used in an STR amplification reaction.

12896MA-W-a

D = degradation target. A DNA sample was serially diluted to low template concentrations and quantified in quadruplicate using the PowerQuant® System and were also amplified in quadruplicate using the PowerPlex® Fusion System.

12896MA-W-b

Representative PowerPlex® Fusion data obtained with DNA template. Casework samples often contain DNA mixtures from multiple persons. The Y-chromosomal target in the PowerQuant® System enables quantification of human male DNA in the sample, helping you evaluate mixtures of male and female DNA. The PowerQuant® System also allows you to make better decisions when faced with a partial or failed STR amplification by differentiating between PCR inhibition and DNA degradation.

Reliably Detect PCR Inhibitors

Reliable detection of inhibitors that can affect DNA quantification and downstream STR assays is a key feature of the PowerQuant® System. The internal PCR control (IPC) has roughly the same level of sensitivity to inhibitors as currently available downstream STR assays, allowing you to determine if a sample is appropriate for amplification or if additional processing is required.

IPC Cq shift predicts downstream STR inhibition.

In contrast to the sample with the lower humic acid concentration, the IPC Cq shifts for the samples with higher humic acid concentration (400ng/μl and 600ng/µl) indicate the presence of an inhibitor and concomitantly show a significant reduction in STR peak heights in the PowerPlex® Fusion System electropherograms. In this example, the [Auto]/[D] ratios are not affected at these humic acid concentrations. This illustrates that the IPC is more sensitive to inhibitors than the degradation target, enabling distinction between sample inhibition and degradation.

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Assess DNA Integrity of Your DNA Sample

The system amplifies short amplicons that are similar in size to mini-STRs and a long autosomal target. After amplification, the PowerQuant® Analysis tool calculates the ratio of DNA concentrations for the shorter and longer amplicons. The resulting degradation ratio helps you determine if a DNA sample is degraded, rather than inhibited, and decide which, if any, STR system is most appropriate for downstream analysis. The experiment below illustrates that the [Auto]/[D] ratio is more sensitive to sample integrity than the IPC, enabling you to distinguish between sample degradation and inhibition.

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ID_Automation

Want to Automate?

Save time and improve precision by automating your DNA quantification set up. The PowerQuant® and Plexor® HY Systems can be automated on the Biomek® 4000 (Beckman Coulter), Freedom EVO (Tecan), or MicroLab STAR (Hamilton) liquid handlers. Additionally, import the quantification data into the STR Normalization Manager Software to automate your PCR amplifications set up.

Automate your DNA Quantification Set up

Contact your local representative to receive a quote for the required quantities and to purchase and schedule the required installation and training services.

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Hardware
Product Cat.#
Four-Position Tube Holder
Plate Clamp 96
Plate Stand

Protocols

Specifications

You are viewing: PQ5002 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

PowerQuant® Male gDNA Standard

DD302A 1 × 150μl View Product

PowerQuant® 20X Primer/Probe/IPC Mix

DK930A 1 × 220μl

PowerQuant® 2X Master Mix

DP527A 2 × 1.1ml

Water, Amplification Grade

DW099A 2 × 1,250μl View Product

PowerQuant® Dilution Buffer

Y101A 2 × 1.5ml

SDS

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Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: PQ5008 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

PowerQuant® Male gDNA Standard

DD302A 3 × 150μl View Product

PowerQuant® 20X Primer/Probe/IPC Mix

DK930A 4 × 220μl

PowerQuant® 2X Master Mix

DP527A 8 × 1.1ml

Water, Amplification Grade

DW099A 5 × 1,250μl View Product

PowerQuant® Dilution Buffer

Y101A 8 × 1.5ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Specifications

You are viewing: DS1221 Change Configuration

What's in the box?

Item Part # Size

PowerQuant® Calibration Standard, FAM

DS106A 1 × 60μl

PowerQuant® Calibration Standard, CFG540

DS107A 1 × 60μl

PowerQuant® Calibration Standard, TMR

DS108A 1 × 60μl

PowerQuant® Calibration Standard, Q670

DS109A 1 × 60μl

PowerQuant® Calibration Standard, CXR

DS110A 1 × 60μl

PowerQuant® Calibration Buffer

DS111A 1 × 30ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Patents and Disclaimers

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Resources

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