The PowerPlex® S5 System is a mini-STR kit that allows co-amplification and detection of four STR markers (D18S51, D8S1179, TH01 and FGA) plus Amelogenin. Full DNA profiles can be achieved with as little as 50pg of DNA and in the presence of 130μM hematin, 200ng tannic acid or 150ng humic acid. The PowerPlex® S5 System is more tolerant of DNA degradation and less sensitive to inhibitors.
This easy to use system comes complete with premixed primer pairs, a master mix with Taq DNA polymerase and internal lane standard. The simplified thermal cycling protocol requires no ramping, and the system is compatible with a number of instrument platforms, including ABI PRISM® 310, 3100 and 3100-Avant and Applied Biosystems® 3130 and 3130xl Genetic Analyzers. Panels and bins text files are required for use with GeneMapper® ID and GeneMapper® ID-X software and are available for download.
One primer specific for each of the Amelogenin, D18S51 and D8S1179 loci is labeled with fluorescein (FL), and one primer specific for each of the TH01 and FGA loci is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE). All five loci are amplified simultaneously in a single tube and analyzed in a single injection. The four STR loci are included in the CODIS and European databases. The amplicons for all loci are smaller than 260bp.
The PowerPlex® S5 System is primarily a screening tool but also can be used as a mini-STR casework kit. Use the system to process many samples to exclude known individuals, primarily in casework scenarios to eliminate samples from the victim. You can also screen people in limited geographical areas to eliminate suspects of a crime perpetrated in a town, village or building or screen samples from large-scale catastrophes. The PowerPlex® S5 System can also amplify small STRs, which amplify more efficiently, and capture large alleles that might drop out with megaplex kits.