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PowerPlex® S5 System

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PowerPlex® S5 System

Sensitive, Easy to Use STR Kit

The PowerPlex® S5 System is a mini-STR kit that allows co-amplification and detection of four STR markers (D18S51, D8S1179, TH01 and FGA) plus Amelogenin. Full DNA profiles can be achieved with as little as 50pg of DNA and in the presence of 130μM hematin, 200ng tannic acid or 150ng humic acid. The PowerPlex® S5 System is more tolerant of DNA degradation and less sensitive to inhibitors.

This easy to use system comes complete with premixed primer pairs, a master mix with Taq DNA polymerase and internal lane standard. The simplified thermal cycling protocol requires no ramping, and the system is compatible with a number of instrument platforms, including ABI PRISM® 310, 3100 and 3100-Avant and Applied Biosystems® 3130 and 3130xl Genetic Analyzers. Panels and bins text files are required for use with GeneMapper® ID and GeneMapper® ID-X software and are available for download

One primer specific for each of the Amelogenin, D18S51 and D8S1179 loci is labeled with fluorescein (FL), and one primer specific for each of the TH01 and FGA loci is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE). All five loci are amplified simultaneously in a single tube and analyzed in a single injection. The four STR loci are included in the CODIS and European databases. The amplicons for all loci are smaller than 260bp.

The PowerPlex® S5 System is primarily a screening tool but also can be used as a mini-STR casework kit. Use the system to process many samples to exclude known individuals, primarily in casework scenarios to eliminate samples from the victim. You can also screen people in limited geographical areas to eliminate suspects of a crime perpetrated in a town, village or building or screen samples from large-scale catastrophes. The PowerPlex® S5 System can also amplify small STRs, which amplify more efficiently, and capture large alleles that might drop out with megaplex kits.

Sensitivity of the PowerPlex® S5 System

7401TA-W
PowerPlex® S5 profile obtained from 63pg template DNA. One microliter of the amplification was analyzed on an Applied Biosystems 3130xl Genetic Analyzer with a 3kV, 5-second injection.

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

2800M Control DNA

DD710A 1 × 25μl10ng/μl View Product

PowerPlex® S5 Allelic Ladder Mix

DG100B 4 × 25μl

Internal Lane Standard 600

DG107A 4 × 150μl View Product

PowerPlex® S5 10X Primer Pair Mix

DK111B 4 × 250μl

PowerPlex® S5 5X Master Mix

DP100A 4 × 500μl

Water, Amplification Grade

DW099A 10 × 1,250μl View Product

SDS

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Download SDSPDF (353 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

BB

Resources

Software and Firmware

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