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GloMax® 20/20 Luminometer


Ultrasensitive Single Tube Luminometer

  • Two fluorescence modules available
  • Optional single or dual auto-injectors
  • Ideal for use with our bioluminescent cell health and gene reporter assays

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Catalog number selected: E5311

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GloMax® 20/20 Luminometer
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Ultra-Sensitive and Versatile Single Tube Luminometer

The GloMax® 20/20 Luminometer combines instrumentation and software in a complete solution that includes bioluminescent assays, protocols and support. In addition to high performance, the GloMax® 20/20 blends user-friendly operation and a small footprint with flexible purchasing options. The result of this design is an ultra-sensitive instrument with superior performance that is easy to use, affordable, and can be customized to your lab's needs.

  • Designed for use with any Promega bioluminescent assay
  • Touch screen interface provides comprehensive instrument control and data collection. 
  • Optional modules for fluorescence detection provide additional flexibility
  • Optional auto-injection system for added convenience meets the demands of the Dual-Luciferase® Assay



  • Plug-and-play usability
  • Preloaded protocols for easy implementation
  • Simple touch screen interface
  • Record data to printer or export to Excel® in real-time
  • Software wizard for setting up new protocols
  • Accessible injector system allows inspection of tubing and tips

  • Optional single or dual auto-injectors; measure flash or glow-type luminescence
  • Two fluorescent modules available
  • Accommodates 1.5ml microfuge tubes and 35mm petri dishes, with optional 12mm × 50mm test tube adapter
  • Measure light output from liquid and solid samples; no minimum volume requirements
Superior Sensitivity

  • Highest instrument sensitivity to measure the lowest assay signals
  • Low cross-talk between wells gives you better, more usable results
  • Broad dynamic range extends linear range of your assay; measure dim and bright samples without dilution

  • IQ/OQ Service available
  • Provides the required technical elements of a part 11 compliant system when used with the appropriate laboratory workflow:
    • User Authentication and Authorization
    • Data Integrity and Protection
    • Electronic Signatures
    • Audit Trails

Design Features

Instrument Specifications

To achieve the GloMax®-20/20's high sensitivity, the luminescence channel is positioned directly below the sample well. These conditions maximize light capture. In addition, a low-noise photomultiplier tube ensures that collected light is not compromised. The dynamic range of the GloMax®-20/20 is more than adequate to cover common luminescence applications, thus reducing the need to dilute samples.

The GloMax®-20/20 Luminometer Light Standard provides an external control to verify performance. Some labs require this additional verification procedure. Reading the light standard before taking measurements is a quick and easy way to ensure quality control of reproducibility, sensitivity, and linearity of measurements.

Detection Modes Luminescence, Fluorescence (optional)
Linear Dynamic Range >8 logs
Read Type Glow, Flash, Kinetic, Repeat
Sample Format 1.5ml microcentrifuge tubes, 35mm petri dishes, 12mm × 50mm test tubes (optional), minicell vials (optional, Fluorescence)
Sample Adapter Holds 1.5ml microcentrifuge tubes, optional 12mm × 50mm test tube holder available.
User Interface Touch screen navigation and operation
Data Output Data displayed on screen or connect to PC (not included) via serial cable to download. Optional thermal printer available.
External PC Requirements (optional) Compatible with 32- and 64-bit PCs using Windows® 7 Professional Service
Pack 1 with either Microsoft® Office 2010 Home and Business Edition (32- and 64-bit versions) or Microsoft® Office 2007 (32-bit version)
Computer Interface 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud rate
Power 12V DC 1.5A Max
Auto Shutoff Hibernates after 15 minutes of inactivity
Dimensions 12.92" D × 10.44" W × 8.42" H (32.82cm D × 26.52cm W × 21.39cm H)
Weight 8.4lbs (3.81kg)
Operating Temperature 60–105ºF (15–40ºC)
Warranty One year
Approvals CE
Single Auto Injector System Specifications
Number of Injectors One injector
Dispense Volume Range Selectable between 25–300µl in 5µl increments
Dual Auto Injector System Specifications
Number of Injectors Two injectors
Dispense Volume Range Selectable between 25–300µl in 5µl increments
Fluorescence Module Specifications

Optional Fluorescence Modules add functionality to the GloMax®-20/20. The modules are designed to measure common fluorescence applications using excitation wavelengths in the near UV or Blue light range. The Fluorescence Modules provide sensitive measurements of DNA, RNA, or protein quantitation dyes.

Light Sources Wavelength-matched LED
Detector PMT
UV Fluorescence Module
Wavelengths Ex 365nm, Em 440–470nm
Typical Fluorophores Hoechst dye, 4-methyl-umbelliferone (4-MU)
Detection Limit 10ng/ml dsDNA using Hoechst Dye 33258
Blue Fluorescence Module
Wavelengths Ex 460nm, Em 515–575nm
Typical Fluorophores EGFP, rAcGFP (DNA Quantitation, RNA Quantitation); Fluorescein (Protein Quantitation)
Detection Limit 450pg/ml dsDNA with DNA Quantitation Dye
Linear Dynamic Range 5 logs, assay dependent
Sample Adapter 100–200µl minicell vial
Read Out Fluorescence Standard Units, Direct Concentration
Calibration Single-point calibration
Discrete Sample Average Sample readings averaged over 5 seconds to improve accuracy

Available Separately

Product Catalog #
GloMax® 20/20 Light Standard E5341
GloMax® 20/20 Fluorescent Module, UV E5351
GloMax® 20/20 Fluorescent Module, Blue E5361
GloMax® 20/20 Test Tube Holder (1.5ml Microcentrifuge Tubes) E5371
GloMax® 20/20 Replacement Tubing (2), Valves (4), Tips (30) E4851*
GloMax® 20/20 Replacement Valves (4 sets) E5391
GloMax® 20/20 Replacement Power Supply E5411
Thermal Serial Printer and Universal Power Cable E2821
Thermal Printer Paper E2851
GloMax® 20/20 Sales Rental E5303
GloMax® 20/20 Base Instrument Service Agreement SA3000
GloMax® Injectors Service Agreement, 1 year SA3040

*Cat.# E4851 has replaced Cat.# E5381.


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