Premier Protein Capture Technology Enables Rapid Capture of Interacting Proteins from Live Cells
Precise identification of proteins that interact with target compounds is made possible using HaloTag® technology—a comprehensive platform for functional protein analysis.
- Potency and permeability of tagged small molecules remains unchanged. Interacting targets are captured using a novel chloroalkane tag, which has minimal effect on compound potency and permeability.
- Protein targets are captured in live cells, ensuring relevancy of results. Modified compounds engage with their targets inside cells, so interactions occur under conditions relevant to the desired cellular phenotype.
- Enrichment method allows capture of low-abundance and low-affinity targets. Enrichment of interacting targets is achieved through efficient capture of the chloroalkane-tagged compound onto immobilized HaloTag® Protein. The rapid and selective nature of the process facilitates identification of low-affinity or low-abundance targets.
Read more about the technology in the paper Deciphering the cellular targets of bioactive compounds using a chloroalkane capture tag. Ohana, R.F., et al. (2015) ACS Chem Biol. 10(10), 2316-24.
Collaborative and Customizable
The service is customizable to your needs. You get access to the protein purification expertise of Promega scientists and the expert mass spec services of MS Bioworks, freeing up your team to concentrate on target optimization. Our stepwise analysis process uses your target compounds and your cell lines, and can be adjusted at any time.
The mass spectrometry expertise of MS Bioworks is applied to analyze and identify the HaloTag®-captured proteins. Through this service you get the people who know most about mass spec working for you. You get the data, and are free to proceed with optimization.