We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Wizard® Plus Midipreps DNA Purification System

A7640

Up to 200µg Plasmid DNA from 10–100ml Cultures

  • Rapid, 90-minute protocol
  • Resin-based batch column method
  • No organic extractions

Size

Catalog number selected: A7640

$ 243.00 Your price: Log In

Add to Helix
Wizard® Plus Midipreps DNA Purification System
25 preps
$ 243.00
Your price: Log In

The Wizard® Plus Midipreps DNA Purification System isolates plasmid DNA in 90 minutes or less, yielding up to 200μg of high-quality DNA with no organic extractions or ethanol precipitations. Multiple midipreps can be easily processed at one time with the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the Wizard® Midicolumn in Nuclease-Free Water (Cat.# P1193). The purified plasmid can be used directly for automated fluorescent DNA sequencing or restriction digestion without further manipulation and also can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).

Plasmid DNA is of comparable quantity and quality to that obtained using time-consuming cesium chloride:ethidium bromide gradient techniques. The purified DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector in BigDye® terminator sequencing.

Wizard® Plus Midipreps requires the use of a vacuum source and either a lab-assembled or commercial manifold, such as the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231) for up to 20 samples or the Vac-Man® Jr. Manifold (Cat.# A7660) for 1 or 2 samples.

Protocols

Specifications

What's in the box?

Item Part # SizeAvailable Separately

Cell Resuspension Solution (CRA)

A711F 1 x 75ml View Product

Cell Lysis Solution (CLA)

A712F 1 x 75ml View Product

Neutralization Solution (NSA)

A7131 1 x 150ml

Wizard® Midipreps DNA Purification Resin

A770A 1 x 250ml View Product

Column Wash Solution (CWB)

A8102 1 x 125ml

Column Wash Solution (CWB)

A810F 1 x 230ml View Product

Wizard® Midicolumns

A765A 1 x 25 each View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Patents and Disclaimers

U.S. Pat. No. 5,808,041.

Resources

Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom