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Wizard® Plus Maxipreps DNA Purification System

A7270

300µg–1mg Plasmid DNA from 100–500ml Cultures

  • 3-hour protocol
  • Resin-based batch-binding and column-washing method
  • No organic extractions

Size

Catalog number selected: A7270

$ 193.00 Your price: Log In

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Wizard® Plus Maxipreps DNA Purification System
10 preps
$ 193.00
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The Wizard® Plus Maxipreps DNA Purification System provides a rapid resin-based batch column method for purification of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. This system typically yields up to 500μg of high-copy-number plasmid DNA (200–20,000bp) from a 100–500ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).

Protocols

Specifications

What's in the box?

Item Part # SizeAvailable Separately

Cell Resuspension Solution

A711C 1 × 150ml View Product

Cell Lysis Solution

A712D 1 × 150ml View Product

Neutralization Solution

A713C 2 × 150ml View Product

Maxi/Megapreps Filtering System

A742D 2 × 5 pack View Product

Wizard® Maxipreps DNA Purification Resin

A769A 1 × 100ml View Product

Column Wash Solution

A810C 1 × 125ml View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Patents and Disclaimers

U.S. Pat. No. 5,808,041.

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