We believe this site might serve you best:

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Custom Maxwell® HT Automated Nucleic Acid Purification Systems

36488360-topimage-CustomMaxwell-HTIMG_3480

Adaptable Purification Capabilities to Respond to Your Evolving Needs

  • Implementation, optimization and automation of Promega technologies on your instruments
  • Custom chemistries for isolation of circulating cell-free DNA, viral total nucleic acid, or RNA
  • Customized training, consultation and assistance
Custom Maxwell® HT Automated Nucleic Acid Purification Systems

Trusted Maxwell® Chemistries for Your High-Throughput Purification Workflows

Take your laboratory's automated purification to new levels with Maxwell® chemistries for high-throughput nucleic acid extraction. Let us help you develop and implement a purification solution that meets your laboratory's workflow needs.


Why Maxwell® HT?

Maxwell® HT chemistries allow automated extraction of nucleic acids from a range of sample types. Based on the successful chemistry contained in Maxwell® prefilled reagent cartridges, Maxwell® HT Systems allow purification of DNA or RNA at scale on laboratory liquid handlers in 24- or 96-well SLAS format. The easy-to-use method produces the highest quality DNA and RNA, and ensures consistent, reliable results in downstream assays.

Maxwell® purification chemistries use novel magnetic particle-based solutions that naturally decrease contamination carryover.

 

Automated DNA and RNA Purification_36488360-overview-MG_8035
36488360-break-MG_3491

Custom Nucleic Acid Purification Chemistries Currently Available

We currently offer automated chemistries for isolation of circulating cell-free DNA isolation of viral total nucleic acid, and RNA purification.

Maxwell® HT ccfDNA Kit
Maxwell® HT Viral Total Nucleic Acid Kit
Maxwell® HT simplyRNA Kit

High-throughput purification of high-quality, amplifiable ccfDNA

The Maxwell® HT Circulating Cell-Free DNA Kit is designed for optimal high-throughput, automated purification of circulating cell-free DNA (ccfDNA) from up to 96 samples of plasma with input volumes of 1–8mls. Protocols are available for the Hamilton® Microlab STAR and Thermo Scientific™ KingFisher™ Flex.

13589MA-W

Here, circulating cell-free DNA was purified from 1–8ml plasma samples using the Maxwell HT ccfDNA custom chemistry automated on a Hamilton STAR® and the Qiagen QIAmp® Circulating Nucleic Acid Kit; Qiagen cannot process 8ml. ccfDNA was quantitated by qPCR. Dye-based fluorescence can overestimate the amount of small DNA present due to the presence of large DNA or RNA carrier molecules.

The Maxwell® HT Viral Total Nucleic Acid Kit is designed for high-throughput automated extraction of viral total nucleic acid (both DNA and RNA) from serum, plasma and other samples. Protocols are available for Beckman Biomek® 4000/FX/NX, Eppendorf epMotion® Series, Tecan Freedom EVO®, Thermo Scientific™ KingFisher™ Flex and Hamilton® Microlab STAR.

13590MA-W
13591MA-W

5,000 IU/ml of inactivated HIV-1 or 500 IU/ml of inactivated hepatitis B virus (HBV) were isolated from 300 μl sample of human plasma using the Maxwell® HT Viral TNA chemistry custom automated on a Tecan Freedom EVO®. Quantitation was performed with PathID® RT-qPCR (HIV-1) and Plexor® RT-qPCR (HBV) using a single-stranded oligonucleotide standard. These proof of concept data were presented as a scientific poster at the 27th Clinical Virology Symposium in 2011.

The Maxwell® HT simplyRNA kit is designed for RNA purification from a variety of sample types to include, but not limited to blood, cells and tissue (fresh/frozen) by high throughput, robotic liquid handler. Protocols are available for Beckman Biomek® 4000/FX/NX, Eppendorf epMotion® Series, Tecan Freedom EVO®, Thermo Scientific™ KingFisher™ Flex and Hamilton® Microlab STAR.

13593MA-W
13594MA-W

Following processing on the Biomek® 4000 Laboratory Automation Workstation with the magnetic particle-based purification technology, RNA from each tissue type (N=3 or 4) was analyzed using the NanoDrop 1000 spectrophotometer. >8ug of RNA was recovered from kidney, liver, and spleen tissue. The RNA was of high purity as denoted by the A260/A280 and A260/A230 purity ratios. Exceptions were A260/A230 ratios for brain (lipid-rich) and heart (fibrous) tissue, although this slight decrease in purity had no effect in RT-qPCR analysis.

36488360-helpsection-MG_3480

How Can We Help You?

Promega Client Representatives, in combination with our Field Support Scientists, will develop an optimized high-throughput solution specifically for you. We can implement Maxwell® HT chemistries on a variety of sample types and instrumentation platforms.

36488360-icon-step1

Step 1

 Automation Consultation
We consult with you to deeply understand your unique needs.

36488360-icon-step2

Step 2

Statement of Work Proposal
Our FSS team will draft a Statement of Work (SOW) to ensure we are aligned with your needs.

36488360-icon-step3

Step 3

On-site Implementation
We schedule our onsite work so that it best fits your schedule.

Please contact us for more information regarding additional sample types and opportunities for customization of this chemistry to meet your laboratory's needs.

For Research Use Only. Not for Use in Diagnostic Procedures.

workflow-background
Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom