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ReliaPrep™ FFPE gDNA Miniprep System

A2351

Intact, Amplifiable DNA from FFPE Tissue

  • Minimal preparation time: simply add ethanol and go!
  • Safely deparaffinize without using harsh organic solvents
  • No overnight digestion required

Size

Catalog number selected: A2351

$ 94.00 Your price: Log In

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ReliaPrep™ FFPE gDNA Miniprep System
10 reactions
$ 94.00
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Higher quality DNA from FFPE samples

ReliaPrep™ FFPE gDNA Miniprep System provides a convenient method for purifying genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Extraction of DNA from FFPE tissues is challenging because the formalin fixation process results in cross-linking between proteins and DNA, as well as between different strands of DNA. This nonoptimal preservation of genomic DNA in FFPE complicates its use in many standard downstream analysis applications.

The ReliaPrep™ FFPE gDNA Miniprep chemistry is optimized to partially reverse this cross-linking without the need for an overnight digestion. The protocol also incorporates a deparaffinization method that does not use xylene or other hazardous or volatile solvents.

The protocol is complete in 2.5 hours with minimal hands-on time.

ReliaPrep™ FFPE Miniprep Systems Help Preserve DNA Integrity

In this experiment, 10µl gDNA purified from FFPE human liver samples was used as template in multiplex PCR to amplify fragments of the GAPDH gene. DNA fragments ranging from 100-400bp were detected.

10142TB-BLKtype

Purified FFPE DNA is Compatible with Sensitive Assays

Here, genomic DNA was purified from 10µm human liver FFPE sections using the ReliaPrep™ FFPE gDNA Miniprep System, and STR analysis performed using the Microsatellite Instability (MSI) Analysis System v. 1.2 (Cat.# MD1641).

10187ta

Simple Protocol

10146ME-W

Specifications

You are viewing: A2351 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Collection Tubes

A130C 1 × 10/pk

Mineral Oil

A244A 1 × 5ml

ReliaPrep™ FFPE Binding Columns

A247A 1 × 10/pk

Proteinase K (PK) Solution

A505A 1 × 250μl View Product

RNase A Solution

A797D 1 × 200μl4mg/ml View Product

Elution Buffer

A828C 1 × 15ml View Product

Lysis Buffer (LBA)

Z101A 2 × 1ml

BL Buffer

Z103A 1 × 3.25ml

Wash Solution

Z104A 1 × 3ml

Blue Dye

MC122A 1 × 100μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: A2352 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Collection Tubes

A130A 2 × 50/pk

Mineral Oil

A244B 1 × 50ml

ReliaPrep™ FFPE Binding Columns

A247B 2 × 50/pk

Proteinase K (PK) Solution

A505B 2 × 1.1ml View Product

RNase A Solution

A797C 1 × 1ml4mg/ml View Product

Elution Buffer

A828C 1 × 15ml View Product

Lysis Buffer (LBA)

Z101B 1 × 30ml

BL Buffer

Z103B 1 × 32.5ml

Wash Solution

Z104B 1 × 30ml

Blue Dye

MC122A 1 × 100μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD
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