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The Maxwell® RSC ccfDNA Plasma Kit is used with the Maxwell® RSC Instruments to purify circulating cell-free DNA (ccfDNA) from 0.2–1.0ml plasma samples. The Maxwell® RSC Instrument processes from 1 to 16 samples, and the Maxwell® RSC 48 Instrument processes from 1 to 48 samples, in approximately 70 minutes. The kit contains everything necessary for purification, with no preprocessing required. You simply add the plasma samples to the prepared cartridges and press Start.
The purified cell-free DNA is of high-quality with little genomic DNA contamination, and is ready for use in downstream applications such as qPCR, next generation sequencing and digital PCR.
Because the Maxwell® RSC is a magnetic particle mover, not a liquid handler, it offers advantages over other automated systems. There is minimal risk of cross-contamination because no liquid handling or splashing happens during sample processing. With no clogs and fewer breakdowns, there are fewer disruptions to your workflow.
The prefilled cartridges and absence of preprocessing steps makes it easy to purify high-quality ccfDNA with a simple three step protocol. In contrast, the lengthy Qiagen protocol requires multiple complicated processing steps.
Simple Protocol saves time. Side-by-side comparison of the protocol steps for the Maxwell® RSC ccfDNA Plasma Kit and the QIAamp® Circulating Nucleic Acid Protocol.
High-Quality ccfDNA is ready-to-use in downstream assays including digital PCR and NGS.
Circulating cell-free DNA yields are equivalent to those from the QIAmp® Kit. DNA was purified from 1ml of plasma using the Maxwell® RSC ccfDNA Plasma Kit and the Qiagen QIAmp® Circulating Nucleic Acid Kit. Isolated ccfDNA was eluted in 80µl and yield was quantified by qPCR.
The seal length on Maxwell® Cartridges has changed to create a pull-tab feature. The manufacturing procedures and quality-control testing of products have not changed.
If you have any questions, please contact us: In North America, call Promega Technical Services at 800-356-9526.Outside North America, contact your branch office or call Promega Technical Services at 608-274-4330.Worldwide, e-mail Promega Technical Services at:email@example.com
Maxwell® RSC ccfDNA Plasma Kit Technical Manual
PDF (545 KB)
Maxwell RSC ccfDNA Plasma Kit Quick Protocol FB212
PDF (190 KB)
Maxwell® RSC Cartridge (RSCG)
Elution Tubes (0.5ml)
Maxwell® RSC Plunger Pack
PDF (0 B)
U.S. Pat. No. 6,855,499 and other patents.
Benchtop instrument for automated nucleic acid purification with integrated quantitation. For Research Use Only.
Benchtop instrument for automated nucleic acid purification of 1–48 samples. For Research Use Only.
Automated Maxwell® sample preprocessing and post-extraction sample preparation for quantitation, normalization and amplification setup.
AS9100, AS9200, AS9101, AS9201
Two fluorescence channels for nucleic acid and protein quantitation.
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