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MagneSil® Genomic, Large Volume System

Scalable DNA Extraction from Large-Volume Samples

  • Walkaway automation from blood-collection tube to application-ready DNA
  • Scale sample size from 1–10ml, batch size from 1–96 samples and reagent usage from input sample volume
  • Validated automated methods available

Size

Catalog number selected: A4082

$ 699.00 Your price: Log In

MagneSil® Genomic, Large Volume System
48 preps
$ 699.00
Your price: Log In

Efficient, Automated DNA Purification from Blood and Other Sample Types

The MagneSil® Genomic, Large Volume System, is designed for scalable, automated genomic DNA isolation from large-volume samples, eliminating laborious centrifugation steps and the use of hazardous organic solvents.

The system has been automated on the Tecan Freedom EVO® liquid handler, providing walkaway purification of genomic DNA from a variety of starting materials, including 1–10ml whole blood samples, regardless of sample storage or shipping conditions. One reagent system and automated method provide yield and purity from any sample type (fresh or frozen blood, samples of unknown quality and mixed sample populations). The instrument uses only the amount of reagents required to process each sample, maximizing efficiency and value per prep.

Magnetic-Based Method Generates PCR-Ready DNA

The MagneSil® Genomic, Large Volume System uses a robust automated method to purify genomic DNA from fresh, frozen or mishandled blood and other samples with similar yields and quality. The system bypasses many of the challenges of centrifugation-based methods by lysing the entire whole blood sample and then directly capturing total genomic DNA from the lysed sample using MagneSil® Paramagnetic Particles (PMPs). The genomic DNA bound to the MagneSil® PMPs is washed to remove contaminants such as heme and cellular proteins, then eluted into an aqueous solution ready for use in downstream applications. There is no need for tedious and lengthy DNA rehydration. The purified genomic DNA is suitable for a variety of downstream applications such as single and multiplex PCR, restriction digestion and real-time PCR.

Optimized protocol is available for the Tecan Freedom EVO® instrument. This and other validated automated methods are available at: www.promega.com/automethods/

Specifications

What's in the box?

Item Part # SizeAvailable Separately

MagneSil® Paramagnetic Particles

A220C 2 × 94ml View Product

eLysis Buffer, Large Volume System

A4091 1 × 1L

Alcohol Wash

MD141E 2 × 500ml View Product

Elution Buffer

MD142C 1 × 300ml View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Patents and Disclaimers

U.S. Pat. Nos. 6,027,945 and 6,368,800, Australian Pat. No. 732756 and other patents and patents pending.

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