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XhoI

R6011

  • Capable of digesting DNA in 15 minutes or less
  • Active and capable of digestion directly in GoTaq® Green Master Mix
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Genome qualified to ensure optimal performance in genomic analysis applications

Catalog number selected: R6161

Cut Site:

CTCGA  G
G  AGCTC

Usage Note: Ends generated with XhoI can be directly ligated to ends generated by SalI. Neither recognition site is regenerated in the ligation product.

Incubation Conditions: Buffer D. 37°C.

Source: Xanthomonas holcicola.

Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM KCl, 0.1% Triton® X-100, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A B C D MULTI-CORE™
25–50% 75–100% 75–100% 100% 10–25%

Frequency of Cutting:

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
1 6 1 0 0 0
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