RsaI

Cut Site:

GT^▼AC
CA_▲TG

Note: For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.

Incubation Conditions: Buffer C. 37°C.

Source: Rhodopseudomonas sphaeroides.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A	B	C	D	MULTI-CORE™
75–100%	75–100%	100%	<10%	<10%

Frequency of Cutting:

λ	Ad-2	ΦX174	pUC18	M13mp18	pBR322
113	83	11	3	19	3

Protocols

Complete Protocol

Restriction Enzyme ProtocolPDF (986 KB) – English

What's in the box?

Item	Part #	Size	Concentration	Available Separately
Buffer C 10X Buffer	R003A	1 × 1ml
Bovine Serum Albumin, Acetylated	R396D	1 × 150μl	10mg/ml	View Product
RsaI	R637A	1 × 1,000u

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Resources

Other Resources

How to Use Restriction Enzymes: A Resource Guide

Frequently Used With

Blue/Orange Loading Dye, 6X

Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.

G1881

Agarose, LE, Analytical Grade

Reagent for electrophoretic separation of nucleic acids.

V3121, V3125, DV3123

Ethidium Bromide Solution, Molecular Grade

Fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients.

H5041

λ DNA/EcoRI + HindIII Markers

Thirteen fragments of 125–21,226bp generated from Lambda DNA digested with EcoRI and HindIII.

G1731

Wizard® Genomic DNA Purification Kit

Manual, solution-based method for extracting DNA from many sample types.

A1120, A1125, A1620