- Sensitive to overlapping dam methylation.
- MboII produces a single-base overhang, which is difficult to ligate.
Incubation Conditions: Buffer B. 37°C.
Source: Moraxella bovis ATCC 10900.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.