Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

I-PpoI (Intron-Encoded Endonuclease)

Click here to customize this product

  • Genome qualified to ensure optimal performance in genomic analysis applications

Choose a concentration


Catalog number selected: R7031

$ 142.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
I-PpoI (Intron-Encoded Endonuclease)
$ 142.00
Your price: Log in

Cut Site:


Note: I-PpoI recognizes rDNA repeats (approximately 200) in S. cerevisiae chromosome 12, generating 600bp, 400bp and 9kb fragments.

Incubation Conditions: I-PpoI Buffer. 37°C.

Source: I-PpoI is an intron-encoded endonuclease from Physarum polycephalum. The enzyme is produced from a recombinant E. coli strain.

Storage Buffer: 50mM Tris-HCl (pH 7.5), 0.01% NP-40, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

10–25% 25–50% 25–50% 25–50% 100%

Note: A precipitate may be observed after thawing. If detected, heat to 37°C for 3–5 minutes and mix to dissolve. The buffer may appear cloudy after heating; this will not affect the performance of the product.

Frequency of Cutting: No sites are present in any of the commonly used plasmids or vectors. One site is present in chromosome 12 of S. cerevisiae DNA.


What's in the box?

Item Part # SizeConcentrationAvailable Separately

Bovine Serum Albumin, Acetylated

R396A 1 × 1,000μl10mg/ml View Product


R703A 1 × 10,000u

I-PpoI Positive Control Plasmid

R707A 1 × 10μg

I-PpoI 10X Buffer

R994A 1 × 1ml


Choose language:

Download SDSPDF (262 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


Let's find the product that meets your needs.

Talk to a Scientist