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I-PpoI (Intron-Encoded Endonuclease)

  • Genome qualified to ensure optimal performance in genomic analysis applications

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Catalog number selected: R7031

$ 139.00
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This product is discontinued
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I-PpoI (Intron-Encoded Endonuclease)
100–200u/µl/10,000u
$ 139.00
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Cut Site:

CTCTC    TTAAGGTAGC
GAGAGAATT   CCATCG

Note: I-PpoI recognizes rDNA repeats (approximately 200) in S. cerevisiae chromosome 12, generating 600bp, 400bp and 9kb fragments.

Incubation Conditions: I-PpoI Buffer. 37°C.

Source: I-PpoI is an intron-encoded endonuclease from Physarum polycephalum. The enzyme is produced from a recombinant E. coli strain.

Storage Buffer: 50mM Tris-HCl (pH 7.5), 0.01% NP-40, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A B C D I-PPoI
10–25% 25–50% 25–50% 25–50% 100%

Note: A precipitate may be observed after thawing. If detected, heat to 37°C for 3–5 minutes and mix to dissolve. The buffer may appear cloudy after heating; this will not affect the performance of the product.

Frequency of Cutting: No sites are present in any of the commonly used plasmids or vectors. One site is present in chromosome 12 of S. cerevisiae DNA.

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Bovine Serum Albumin, Acetylated

R396A 1 × 1,000μl10mg/ml View Product

I-PpoI

R703A 1 × 10,000u

I-PpoI Positive Control Plasmid

R707A 1 × 10μg

I-PpoI 10X Buffer

R994A 1 × 1ml

SDS

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Download SDSPDF (122 KB) – English (United States)

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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Manas

Manas

India