- For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
- Ends generated by BssHII can be directly ligated to ends generated by Mlu92I. Neither recognition site is regenerated in the ligation product.
Incubation Conditions: Buffer H. 50°C. (BssHII exhibits 75–100% activity at 37°C.)
Source: Bacillus stearothermophilus H3.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 100mM sodium citrate, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.