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Usage Note: Ends generated with BglII can be directly ligated to ends generated with BamHI, BclI and XhoII. These restriction sites are not regenerated in the ligation product.
Incubation Conditions: Buffer D. 37°C.
Source: Bacillus globigii.
Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.