Conventional DNA Digest Markers are created by digesting DNA (i.e., λ DNA, ΦX174 replicative form DNA or plasmids) to completion with one or more restriction enzymes. The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol-precipitated or just ethanol-precipitated. The precipitated fragments are resuspended in storage buffer. These markers are not intended for quantitative analysis.
The ΦX174 DNA/HinfI Markers have 20 ethanol-precipitated DNA fragments ranging in size from 24bp to 726bp.
Storage Buffer: 10mM Tris-HCl (pH 7.5), 1mM EDTA.